Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Agar look ok? Should I be worried about single spore growth?
    #25674822 -

Made some "no pour" agar plates.  They had a lot of condensation until a few days after I removed the foil and inoculated them.

So the drop of MS spore solution I added mixed with the condensation and sploshed around a bit when handling the plate.

Been a week and now my myc looks like a bunch of tiny isolated dots or islands (see image below).

Did an agar image search and found some similar "island" growth.

Someone commented on that pic to say:
"If you spread the spores out like this, they have less chance of fertilizing each other to make a diversity of strains. Also, you won't be able to see clear radial sectoring because they don't all start growing from the center.  Oh, and one of those small perfectly circular islands is probably growth from a single spore, so it will be infertile."

Went on to say you could grow them out and look for sectoring.  If you see sectoring they're ok (i.e. not formed by a single spore / not infertile).

But I've also read it can be hard to get clearly defined sectors without going through multiple transfers / dishes because strains will grow on top of each other making the appearance of strains difficult to see.

And I haven't found any other discussion of infertile, single spore myc so I'm not sure if that's good advice.

So I'm wondering...
(1) should I be concerned about this being infertile, single spore growth?
(2) if so, what's the quickest and / or easiest way to tell if this myc is fertile?



Extras: Filter Print Post Top
Re: Agar look ok? Should I be worried about single spore growth? [Re: mycolojist]
    #25674830 -

i find it easier to keep the spores together on the plate so it has room to grow out


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

Extras: Filter Print Post Top
Re: Agar look ok? Should I be worried about single spore growth? [Re: tripdawg420]
    #25675018 -

None of those spots are single spore growth, just grab whatever looks best, and transfer to a new dish.


--------------------
I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

Extras: Filter Print Post Top
Re: Agar look ok? Should I be worried about single spore growth? [Re: Johnny Dont]
    #25675022 -

Johnny Dont said:
None of those spots are single spore growth, just grab whatever looks best, and transfer to a new dish.


:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

Extras: Filter Print Post Top
Re: Agar look ok? Should I be worried about single spore growth? [Re: tripdawg420]
    #25675442 -

tripdawg420 said:
Johnny Dont said:
None of those spots are single spore growth, just grab whatever looks best, and transfer to a new dish.


:thumbup:


Appreciate the feedback!

Glad they look ok :smile:

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Re: B+ Fruiting On Agar!!! Anonymous 1,853 6 01/12/00 06:39 PM
by Anonymous
* Re: Antibiotic Agar Mix for Spore Germiniation in Water? Anonymous 1,663 1 05/09/00 06:46 PM
by Anonymous
* My agar or grains? please help McGovern453 182 3 12/13/18 01:44 PM
by JHOVA
* Whats with my agar plates? Frankenstein 9,573 8 09/08/22 03:46 PM
by schpat
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )		 funkyballoon 22,731 38 05/11/01 11:08 AM
by MAIA
* Agar and growing that white stuff! pj541 2,793 5 10/15/01 04:06 PM
by pj541
* Benefits of Agar? celsius 9,951 10 12/01/19 12:36 AM
by wildernessjunkie
* Re: How do I use a sporeprint versus the normal spore syringe? Anonymous 2,432 11 07/26/00 09:54 AM
by psilocyber

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
173 topic views. 7 members, 892 guests and 75 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 16 queries.