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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: hamloaf] * 1
    #24336706 -

Nice 1000th post. One day I will have cool toys n stuff. Need to finish fixing my house and then sell it first. Getting close.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Pastywhyte]
    #24336897 -

thanks for the feedback guys :headbang3:

i am gonna go back shortly and add some more on streaking and some more distinctions between SAB and FH technique that i forgot to mention

what toys are on your wish list pasty?


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24336909 -

psh your custom SAB is on my wishlist lol https://www.shroomery.org/forums/showflat.php/Number/23557396/fpart/1/vc/1
the plexi one that is.

I wouldn't do a hood until I could make a room with as close to seamless hygienic floor and walls as I could manage.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bodhisatta] * 1
    #24336938 -

lol that thing was pretty handy,i didnt really take as many pics as i should have, it had a hinged lid with lights built in and shit.. it was surprisingly comfortable for doing bags, i could even do bag to bag in it

i actually found the big piece of plexiglass for that thing on the side of the road haha... my gf thought i was insane the way i pulled a U turn for "a piece of garbage" :lol:

i had around a 99% success rate with plates in that thing after i started using a towel + rack (over 15000 plates)... not so great with jars and bags though... probably sometimes because of how hard i had to struggle to unscrew lids before i got a strap wrench (that thing is clutch). more like 90%

that custom SAB was built into a closet, so that i could close it and it totally disappeared :lol: it was super covert

i still have to build the table for my FH, i plan to build it into a really big closet, with a custom table underneath. my coffee table has an e-nail built in, im gonna build one into this table as well, and lots of storage for tools and stuff :cool:

i expected similar success rates with my FH, but so far over a couple thousand plates i have only seen 1 with ANY contam (and i dropped it on the floor :lol:). ive only made 14 grain jars so far with it, and they all look great. been kinda amazed at how well this thing works, i expected to see at least 1 plate contam every few hundred, ive had to adjust my workflow and do less plates (since i dont have to plan on ANY contaminating)


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o] * 2
    #24336951 -

what do you do with these hundreds of plates if you only did a few grain jars lol.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bodhisatta]
    #24336967 -

My wishlist is for a room without carpet and tile floor to do lab work. Have a flowhood and dissection scope. Erbach would be nice along with a 100 cc borosilicate spring loaded syringe with a 3 inch 14 ga stainless luer lock needle with cross cut tip. A walk in GH would be sweet. You know the little things.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Pastywhyte]
    #24337001 -

bodhisatta said:
what do you do with these hundreds of plates if you only did a few grain jars lol.



only a few grain jars since building my flow hood, ive only had it a few weeks... i moved recently, and unexpectedly, and instead of building a new SAB i built a FH.

i did hundreds in my SABs, and lots of bags. since getting that FH built ive refreshed the cultures i want to work with, started a bunch of new stuff, and continued a bunch of isolation projects (some isolates, some 1 to 80 transfers in)

Pastywhyte said:
My wishlist is for a room without carpet and tile floor to do lab work. Have a flowhood and dissection scope. Erbach would be nice along with a 100 cc borosilicate spring loaded syringe with a 3 inch 14 ga stainless luer lock needle with cross cut tip. A walk in GH would be sweet. You know the little things.



a man of fine tastes :smile: glad i asked, never thought about one of those syringes.. ive been shopping for a dissection scope lately... i think it was mushpunx who said he got an erbach and lab blender for $70!!


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24337022 -

Shit I have been looking at places that custom create syringes. I even priced out one that would be everything I wanted, was over 300 bucks. But the shit would last forever. Maybe when the tax return comes in.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Pastywhyte]
    #24337217 -

You guys be joshin too much


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Boogieman47] * 1
    #24337390 -

Beautiful write up c10! :rockon:

I've only been doing the Pastywhyte Ezi LC jars for the past few months, I've all but forgotten how to do petris :hehehe: You've convinced me to pull the trigger on getting a 500pc order, I was wondering if it was worth it but clearly I need to step up my game.

I'll be following this tek to the T when I do my next batch of petris.

You seriously deserve TC status!

Make
Agar
Great
Again!


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My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

Edited by ManifoldPrime (05/21/17 06:45 AM)

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime] * 1
    #24341493 -

UPDATED.

haha thanks buddy, i appreciate it :smile:

:rofl: MAGA: make agar great again, i fucking love it!! thats going in the subtitle :smile:


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (05/22/17 08:04 PM)

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24341508 -

:takingnotes: phones at 1% so I'll read later. nice new avatar C10 :lol:


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: van hatton]
    #24341540 -

ok i finally read your post.


:rockon:

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: mushboy]
    #24355310 -

:thumbup: good info thanks :thumbup:


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HMU if you have Psilocybe semilanceata, Psilocybe hispanica, Gymnopilus luteofolius, Psilocybe azurescens

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Sappbelly]
    #24374239 -

UPDATED


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24374455 -

Nice post man!


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Amateur Mycologists United
AMU Q&A

Edited by mushpunx (06/03/17 07:01 PM)

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: mushpunx]
    #24375352 -

thanks buddy :smile:

im gonna be updating this again soon with more pics and some additional tips, and possibly some videos :smile:


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24414276 -

I just used that streaking method for the first time, and loosely followed this guide as a refresher to whip up a batch of B+ colonies.

Will post updates ITT.

Thanks C10!


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime]
    #24414558 -

Nice write up man. I'm gonna try that streak pattern tonight I like it. Good info thanks.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Dabadoodledoo710]
    #24423337 -

informative write-up, thanks for mentioning the part about straining the agar for clarity. i had heard of this being done but couldn't find any info on how.


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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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