blackdust said:

Slurry

eatyualive said:

just to show something that will throw many on a loop. this is a 2 quart wbs grain slurry to 9 quarts of verm. this is day 10. 3rd try on the slurries. subs were oven pasteurized. this is uncased. the sub colonized in 3 days and was put into fruiting on day 4.

Substrate is 9 quarts volume.

1 small brick coir
2 quarts verm
a cup of gypsum
5 quarts water

its usually 10 quarts volume for me. what i did was take about a quart of that volume out and use it as casing layers for other tubs.

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eatyualive said:

Inocuole said:

Oh, cool, so there is coir in there.  Whew.  You had me REALLY going for a loop there.

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this is what i did.

GRAIN WAS PREPPED USING FOOMAN'S GRAIN TEK IN 2 HOURS

MADE 40 QUARTS WBS GRAIN JARS FROM 1 PF JAR SLURRY (THAI CLONE FOR A TEST RUN BELOW)



THAI LIPA YAI (TEST CLONE UNCASED TUB):                                             
TUB 1 YIELD: TOTAL 450g 17.9 oz
1st: 240 grams 8.5 oz
2nd: 70 grams 2.5oz
3rd: 59g 2oz
4th: 81g 2.9oz




MIXED UP 1 SMALL BRICKS COIR, 2 QUARTS VERM, 1 CUP GYPSUM, 5 QUARTS WATER

EXPANDED THE COIR WITH HOT WATER

DUMPED IN THE DRY VERM AND MIXED IT ALL UP WITH THE COIR. THEN POURED THE GYPSUM THAT WAS MIXED IN WITH 4 CUPS OF THE WARM WATER ON TOP OF THE VERM COIR MIX. THEN MIXED IT ALL UP.


PUT IT IN TURKEY TINS IN THE OVEN FOR 2.5 HOURS. INNER CORE TEMP WAS BROUGHT TO 170 THEN SET FOR 2 HOURS AT THAT TEMP.


ONCE COOL, FLIPPED THE TURKEY TIN UPSIDE DOWN IN MY PRE LINED TUB.
I TOOK A WIDE MOUTH QUART JAR THAT I RETROFITTED A BLADE ASSEMBLY TO THE LID. I PRESSURE COOKED 3/4 QUART OF WATER AND THE BLADE ASSEMBLY. ONCE COOL, I USED A SHMUVBOX FOR CLEAN WORK AND TOOK THE LID OFF 1 COLONIZED QUART. I POURED HALF OF THE 3/4TH QUART OF WATER INTO THAT COLONIZED QUART AND QUICKLY PUT THE METAL BLADE ON IT. I THEN USED A DRILL BIT ON THE END OF THE BLENDER TO BLEND UP THE JAR. I THEN DID THE SAME THING ON THE 2ND COLONIZED QUART OF GRAIN. I POURED THE REST OF THE WATER INTO THE 2ND QUART, THEN PUT THE SAME LID ON THE 2ND QUART JAR. THEN BLENDED IT WITH THE DRILL BIT. I POURED IT ALL INTO THE SUBSTRATE AND MIXED IT IN THOROUGHLY WITH THE PASTEURIZED SUBSTRATE WITH GLOVES ON.(I DIDN'T TAKE PICS SO IM SUBSTITUTING SOME I HAVE THAT ILLUSTRATE THIS)

BLEND WASN'T QUITE THIS SOUPY BUT DID LOOK SOMEWHAT LIKE THIS.


THE QUART LID IS LIKE THIS.

---

Yeah. Speed is what I like. Lets get rid of this waiting game. Not to mention that using the 9er tek can get a clone to fully colonize a PF cake in 4 days. That clone PF cake can be expanded to over 50 qt jars of grain in another 3 days. LOOOK, 7 days to get 50qts of spawn. blows my fucking mind.

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pablokabute said:

blackdust said:

Slurry

eatyualive said:

just to show something that will throw many on a loop. this is a 2 quart wbs grain slurry to 9 quarts of verm. this is day 10. 3rd try on the slurries. subs were oven pasteurized. this is uncased. the sub colonized in 3 days and was put into fruiting on day 4.

Substrate is 9 quarts volume.

1 small brick coir
2 quarts verm
a cup of gypsum
5 quarts water

its usually 10 quarts volume for me. what i did was take about a quart of that volume out and use it as casing layers for other tubs.

---

eatyualive said:

Inocuole said:

Oh, cool, so there is coir in there.  Whew.  You had me REALLY going for a loop there.

---

this is what i did.

GRAIN WAS PREPPED USING FOOMAN'S GRAIN TEK IN 2 HOURS

MADE 40 QUARTS WBS GRAIN JARS FROM 1 PF JAR SLURRY (THAI CLONE FOR A TEST RUN BELOW)



THAI LIPA YAI (TEST CLONE UNCASED TUB):                                             
TUB 1 YIELD: TOTAL 450g 17.9 oz
1st: 240 grams 8.5 oz
2nd: 70 grams 2.5oz
3rd: 59g 2oz
4th: 81g 2.9oz




MIXED UP 1 SMALL BRICKS COIR, 2 QUARTS VERM, 1 CUP GYPSUM, 5 QUARTS WATER

EXPANDED THE COIR WITH HOT WATER

DUMPED IN THE DRY VERM AND MIXED IT ALL UP WITH THE COIR. THEN POURED THE GYPSUM THAT WAS MIXED IN WITH 4 CUPS OF THE WARM WATER ON TOP OF THE VERM COIR MIX. THEN MIXED IT ALL UP.


PUT IT IN TURKEY TINS IN THE OVEN FOR 2.5 HOURS. INNER CORE TEMP WAS BROUGHT TO 170 THEN SET FOR 2 HOURS AT THAT TEMP.


ONCE COOL, FLIPPED THE TURKEY TIN UPSIDE DOWN IN MY PRE LINED TUB.
I TOOK A WIDE MOUTH QUART JAR THAT I RETROFITTED A BLADE ASSEMBLY TO THE LID. I PRESSURE COOKED 3/4 QUART OF WATER AND THE BLADE ASSEMBLY. ONCE COOL, I USED A SHMUVBOX FOR CLEAN WORK AND TOOK THE LID OFF 1 COLONIZED QUART. I POURED HALF OF THE 3/4TH QUART OF WATER INTO THAT COLONIZED QUART AND QUICKLY PUT THE METAL BLADE ON IT. I THEN USED A DRILL BIT ON THE END OF THE BLENDER TO BLEND UP THE JAR. I THEN DID THE SAME THING ON THE 2ND COLONIZED QUART OF GRAIN. I POURED THE REST OF THE WATER INTO THE 2ND QUART, THEN PUT THE SAME LID ON THE 2ND QUART JAR. THEN BLENDED IT WITH THE DRILL BIT. I POURED IT ALL INTO THE SUBSTRATE AND MIXED IT IN THOROUGHLY WITH THE PASTEURIZED SUBSTRATE WITH GLOVES ON.(I DIDN'T TAKE PICS SO IM SUBSTITUTING SOME I HAVE THAT ILLUSTRATE THIS)

BLEND WASN'T QUITE THIS SOUPY BUT DID LOOK SOMEWHAT LIKE THIS.


THE QUART LID IS LIKE THIS.

---

Yeah. Speed is what I like. Lets get rid of this waiting game. Not to mention that using the 9er tek can get a clone to fully colonize a PF cake in 4 days. That clone PF cake can be expanded to over 50 qt jars of grain in another 3 days. LOOOK, 7 days to get 50qts of spawn. blows my fucking mind.

---

thats fucking crazy.

---

BUK said:

Did you really need to quote all that just to comment?

Anyway, could anyone opine on the speed differences between: agar wedge, G2G, LC, agar slurry, grain slurry

(I'm presuming that's the right order but I've only partial experience)

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MudaFuka said:

An agar wedge is the slowest. When I have inoculated grain jars with a wedge they usually took about 2 weeks to colonise. Agar LI and G2G take about the same amount of time for me it is usually about 8 days give or take.

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Violet said:

I get colonization of grains in just 4-6 days with agar wedges by simply shaking the shit out of them after resealing... LI is definitely the easiest, most consistent, and most spreadable way to achieve that though

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Violet said:

I get colonization of grains in just 4-6 days with agar wedges by simply shaking the shit out of them after resealing... LI is definitely the easiest, most consistent, and most spreadable way to achieve that though

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Mr. Alien said:

Hey guys i think this is a great idea for LI or LC. No syringes.

I was thinking in pouring LI to an autoclavable pp5 wash bottle in a SAB or hood





This way Grain jars inoculation will be mega easy and faster, it won't be a "puoring" it would be more like a "aim and shoot", this means you will have your grain jars less time open/exposed=less contam chances. Also workflow would be faster finishing all your jars inoculations in no time.

The liquid dispenser is so small that you can seal it with parafilm after finishing inoculating all your jars and probably enough for GE too.

Also the dispenser conduct is so small compared to the wide mouth of the mason jars, that you won't need to worry about any contams entering trough the dispenser conduct while you're inoculating in the SAB or flowhood (easier sterile procedures).

Another advantage is that, you have better control of the inoculation amounts depending on how much you squeeze the bottle. 

The only problem would be pouring LI or LC trough the narrow mouth of the wash bottle, but i'm sure there is autoclavables wide mouth wash bottles out there like this one

Guardian187 said:

@Violet -- are you still using the same pint container tek you wrote up a while back?

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Hey Guardian,

You're on the right track.

MudaFuka said:

BUK said:

Did you really need to quote all that just to comment?

Anyway, could anyone opine on the speed differences between: agar wedge, G2G, LC, agar slurry, grain slurry

(I'm presuming that's the right order but I've only partial experience)

---

An agar wedge is the slowest.

---

Guardian187 said:

@Violet -- are you still using the same pint container tek you wrote up a while back?

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