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Selecting a sector, quick question
    #19448371 -

Hey guys, thanks for checking out the post! :rockon:

So first off, I just started working with agar. :cool:
I inoculated a MEA petri with a couple drops of Cambodian MS solution.
The pics below are all 2 transfers from my first plates:
   
                        Front                                                        Back

   
                        Front                                                        Back

   
                        Front                                                        Back
I plan on making transfers from these plates to further isolate healthy looking strains. That's what the numbers indicate... :wink:


But this plate here is making it difficult to decide where to take a sample from:

   
                        Front                                                        Back
:mushroom2:Two questions...
How many sectors you think there are in this culture?
Where would you take the sample from?

Any other Questions or Comments? Post em!
Thanks Guys!:bigjoint:

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Re: Selecting a sector, quick question [Re: Vis0nairee]
    #19448451 -

I'll be doing this soon as well.
:popcorn:

Wish i could give ya more bro....


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~~Everything is relative~~


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Re: Selecting a sector, quick question [Re: Vis0nairee]
    #19448454 -

i'd take from 1-2 o clock on that last "front" picture and see if that sectors.
just a side note...dunno if u do this already, but that parafilm can be cut in half length wise and still be effective.  for example, I take 3 "squares" (a square being one repetition of print on the roll) and cut it in half lengthwise for two dishes, there is less overlap on the top and bottom.  not necessary, but it will save u parafilm!


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: Selecting a sector, quick question [Re: blindingleaf]
    #19448474 -

Like above I would take from the 1-2 position. You probably wont see sectoring yet even after that transfer. You could really take it from anywhere on that dish though.

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Re: Selecting a sector, quick question [Re: bodhisatta]
    #19448508 -

bodhisatta said:
Like above I would take from the 1-2 position. You probably wont see sectoring yet even after that transfer. You could really take it from anywhere on that dish though.



I wanted to wait and see what others thought, but I am on board with the 1:30-2 oclock position, though resign to thinking that from the look it wont matter one bit.

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Re: Selecting a sector, quick question [Re: Vis0nairee]
    #19448531 -

2:00

Take a tiny piece from the leading edge.  Spores, right?

That grown out may/will contain many sectors and it looks like a good starting point.

Good luck, Dr. VisO


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Re: Selecting a sector, quick question [Re: blindingleaf]
    #19448772 -

Thanks for the input guys, I mostly wanted to show peeps what I've been working on.:smile:
After the few transfers I've made, I already feel really confident and comfortable working with agar in my SAB. Agar FTW!:bigblunt:

As for the transfer I'm thinking, oh I dont know... 1:45ish? :grin:

blindingleaf said:
dunno if u do this already, but that parafilm can be cut in half length wise and still be effective.  for example, I take 3 "squares" (a square being one repetition of print on the roll) and cut it in half lengthwise for two dishes, there is less overlap on the top and bottom.  not necessary, but it will save u parafilm!



You've mistaken my Glad Press n' Seal for Parafilm. :shocked: But I appreciate the tip, Im sure someone that uses parafilm does too.
:asianofapproval:

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