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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: 10 PE ISOLATES [Re: first time expert]
    #18280984 -

I hope no one walks in one me while I admire your peen for a few more hours.


--------------------
have a look at my grow.

"genius by day, junkie by night"

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Re: 10 PE ISOLATES [Re: fungitarian]
    #18280999 -

Auto-Summary
The author grew ten different strains of mushrooms using tissue samples from three MS PE fruits. After colonizing master grain jars, the mycelium was expanded via Grain to Grain to 9 quarts each. Subsequently, 8 quarts from each batch were spawned to coir and verm, resulting in successful fruiting for six of the ten isolates. The total yield from the first flush was 41.45 ounces.

Notahacker420 said:
So I took tissue samples from 3 nice MS PE fruits and placed them on agar.

   

After a few transfers, I had 10 plates that looked like this.

   

I then cut a nice wedge from each dish and inoculated 10 master grain jars with them.

Took about 2.5 weeks for full colonization of all jars, shaking each jar once at 20-30%.

Once they were all fully colonized, I expanded the mycellium via G2G(Grain to Grain) to 9 quarts each.

Grains were WBS(Wild Bird Seed) rinsed and soaked in hot tap water for 24 hours, then strained for 20 minutes, loaded into quart jars and PCed for 100 minutes at 15psi.

Once all 90 were fully colonized, 8 quarts from each batch/culture(1-10) were spawned to 1 brick of coir(650g) and 3 quarts of verm following Damion5050's Bucket Tek.

I used 5.5qts of water in my preparation. This equals out to 4qts for the brick of coir, and .5qts for each quart of verm.

They were spawned in monotubs and were all fully colonized in 10 days, and then fruited.(No consolidation)

Spawn was mixed evenly with the substrate with no top layer and have all shown just about equal colonizations speeds.




TUB - ISOLATE #1
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 8.5oz DRY)

TUB - ISOLATE #2 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT!
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22

TUB - ISOLATE #3
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                        (HARVEST 6oz DRY)

TUB - ISOLATE #4 - UNFORTUNATLEY LOST TO TRICH - :sad:
   
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11


TUB - ISOLATE #5 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT!
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22

TUB - ISOLATE #6
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 5.5oz DRY)

TUB - ISOLATE #7 - HIT WITH TRICH/COBWEB - ISOLATED TO FINISH FLUSH
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 5.75oz DRY)

TUB - ISOLATE #8 - HIT WITH TRICH AFTER 3 WEEKS - TOSSED
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22

TUB - ISOLATE #9
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 26
                                                                                                                                      (HARVEST 7.3oz DRY)

TUB - ISOLATE #10
         
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 26
                                                                                                                                      (HARVEST 4.4oz DRY)


RANDOM PICS

   


First Flush Totals:

Blobs      4.00oz
Tub #1    8.50oz
Tub #3    6.00oz
Tub #6    5.50oz
Tub #7    5.75oz
Tub #9    7.30oz
Tub #10  4.40oz 
Total  =  41.45 Ounces of Cock!



now you don't have to jump pages to peek


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: 10 PE ISOLATES [Re: cronicr]
    #18281583 -

:bathtub40lol:

You guys are nuts!   








742 Evergreen Terrace


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: 10 PE ISOLATES [Re: PussyFart]
    #18282552 -

Damn, rhode island is kinda far.


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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Re: 10 PE ISOLATES [Re: zMan]
    #18282675 -

Rhode Island?

:lmafo:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: 10 PE ISOLATES [Re: FrankHorrigan]
    #18283409 -

:awesomenod: I was hoping someone would get it lol.


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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Re: 10 PE ISOLATES [Re: zMan]
    #18347964 -

Hey hacker, what can we get an update on the second flush? Don't need pics, but maybe weights? Did you lose anymore of em? Just getting curious.

I got 6 1/2 ounces from a MS tub through first flush (blobs to dicks)

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Re: 10 PE ISOLATES [Re: CoreyRubble]
    #18348130 -

got to say those fruits make me want the peen :blush: :mushroom2:

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Re: 10 PE ISOLATES [Re: Gr13nMushDude]
    #18348277 -

I might have gotten another 8 ounces total from the rest of the tubs, after the recorded harvest.

They all went downhill pretty fast after the trich infestation.

Everything came out of the closet...bleached the walls and shampooed the carpet...cannot remove the carpet for certain reasons.

Going to get a few air purifiers just to see if it helps the mold spore count in the house.

Going to try true pasteurization here in the next week or so.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: 10 PE ISOLATES [Re: PussyFart]
    #18348288 -

Notahacker420 said:
Going to try true pasteurization here in the next week or so.


Fuck all the rest of that cleaning noise, just vacuum and use proper pasteurization, problems solved :cool:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: 10 PE ISOLATES [Re: FrankHorrigan]
    #18348310 -

:lmafo:  Will do buddy, will do... :thumbup:


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: 10 PE ISOLATES [Re: PussyFart] * 1
    #18350871 -

And all it took was 10 tubs of trich to convince him....


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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Re: 10 PE ISOLATES [Re: zMan]
    #18350886 -

It was more like 4 tubs....I am convinced the rest just contammed because of the massive spore count in the air.

But, I digress...


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: 10 PE ISOLATES [Re: PussyFart]
    #18350964 -

Knock on wood, but none of my bulk subs have seen trich yet. I got trich, or penicillium in a jar once, but thats it. Ive had way more bacterial issues, mostly in my pre agar phases, and one issue of black mold trying the laundry basket tek. I got one of my bulk cakes that seems bacteria infected, and so far has only produced like 6 mushrooms, where its siblings have produced ounces.

But thats not bad for my first 3 cakes.

Ive learned to make slightly smaller cakes, and to pressure cook them longer.


--------------------
1) Everything I ever posted or say is a lie.

Edited by Sillyputty67 (05/31/13 11:27 PM)

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Re: 10 PE ISOLATES [Re: Sillyputty67]
    #18352472 -

I got black mold from dropping an agar wedge and then picking it up and putting it in the jar lol. I never for a sec thought it would make it and exactly where my fingers grabbed is where the mold started.


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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Re: 10 PE ISOLATES [Re: zMan]
    #18353273 -

I killed my first nameko culture the same way. I accidentally dropped the agar wedge on the table, before it made it to the agar. Gray mold.....


--------------------
1) Everything I ever posted or say is a lie.

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Re: 10 PE ISOLATES [Re: Sillyputty67]
    #19140846 -

how am i just now seeing this?

nice write up hax.  kudos

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Re: 10 PE ISOLATES [Re: twistedty]
    #19141897 -

nice bump ty(seriously)


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: 10 PE ISOLATES [Re: cronicr]
    #19142561 -

Thanks.....running 12 tubs of #7 as we speak.....uncased....


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: 10 PE ISOLATES [Re: PussyFart]
    #19142733 -

Notahacker420 said:
Thanks.....running 12 tubs of #7 as we speak.....uncased....


:threadmonitor:


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!

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