iodine

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mad capper
aspiring alchemist

Registered: 06/27/10
Posts: 312
Loc: down south

Last seen: 9 years, 5 months

iodine

#13655577 - 12/19/10 01:19 PM (15 years, 10 months ago)

im getting ready to clone some live tissue on agar. i planned on cleaning the stem with iso alcohol 70% but read somewhere that iodine is good for sterilizion too. Does anyone have a link or info?

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*I told my wife that a husband is like a fine wine; he gets better with age. The next day, she locked me in the cellar.*

Mad Capper's moonshine tek

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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,878
Loc: PNW
Last seen: 1 year, 10 months

Re: iodine

[Re: mad capper]
#13655630 - 12/19/10 01:34 PM (15 years, 10 months ago)

Not necessary if you're cloning mushrooms you grew at home. Search RogerRabbit's posts under "iodine", he has a bunch of posts on using either iodine or diluted sodium hypochlorite (bleach) than rinsing the culture in sterile water. ~P~

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Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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mad capper
aspiring alchemist

Registered: 06/27/10
Posts: 312
Loc: down south

Last seen: 9 years, 5 months

Re: iodine

[Re: Psuper]
#13655747 - 12/19/10 02:03 PM (15 years, 10 months ago)

thanks im just concerned because the tub that im taking the tissue from just started showing green its my only source of live tissue tho

--------------------
*I told my wife that a husband is like a fine wine; he gets better with age. The next day, she locked me in the cellar.*

Mad Capper's moonshine tek

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k00laid
NEMO

Registered: 05/03/10
Posts: 19,636
Last seen: 3 years, 3 months

Re: iodine

[Re: mad capper]
#13655818 - 12/19/10 02:22 PM (15 years, 10 months ago)

alcohol and iodine do not sterilize anything.

you should split the stem and take a tissue sample from the inside which has never been exposed to air. thus being sterile.

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AMU - AMU Q & A - MyVideo Teks!

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mad capper
aspiring alchemist

Registered: 06/27/10
Posts: 312
Loc: down south

Last seen: 9 years, 5 months

Re: iodine

[Re: k00laid]
#13656374 - 12/19/10 04:26 PM (15 years, 10 months ago)

thanks koolaid. I had already planned on doing a biopsy. Basically a variation of the 9er tek and I intended on just cleaning my stem up real good with 70% iso before I started disecting. Like i said my main concern was that my tub was starting to show early signs of trich, and I didn't wanna carry that over into the culture.

--------------------
*I told my wife that a husband is like a fine wine; he gets better with age. The next day, she locked me in the cellar.*

Mad Capper's moonshine tek

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mad capper
aspiring alchemist

Registered: 06/27/10
Posts: 312
Loc: down south

Last seen: 9 years, 5 months

Re: iodine

[Re: Psuper]
#13656468 - 12/19/10 04:49 PM (15 years, 10 months ago)

thanks for the tip psuper

--------------------
*I told my wife that a husband is like a fine wine; he gets better with age. The next day, she locked me in the cellar.*

Mad Capper's moonshine tek

Extras:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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