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OfflineShroomzilla
enthusiast

Registered: 02/04/01
Posts: 177
Loc: Texas, home of the longho...
Last seen: 19 years, 7 months
Re: Alcohol 80% better than 96%!! [Re: paddoholland]
    #270730 - 03/13/01 05:31 AM (20 years, 7 months ago)

Bleach is used by "hazmat' teams because it is cheap and it works, but it is only getting used once in a particular location, and they are wearing respirators to protect from all the nasties.

They use a combination of QT amonium cleaner and UV lights to sterilize in hospitals because you don't want the nasty fumes choking everyone, and you don't want the residue build-up bleach can leave...or the corroded metal fittings.

UV lights BTW, are only used to "maintain" sterility in unused rooms once all surfaces have been cleaned, they are not used for primary disinfection because its too easy for even a thin layer of dust to "shield" pathogens, it also can't get to anything down in any pores/cracks on a surface. One small 25w tube is plenty for an enclosed work box or glove box, but to do a room you need many larger lamps.

You can actually build a quite effictive UV "hepa" filter. the UV tube is centered in a small diameter duct lined with a reflective material and air is passed through at a given volume based on lamp output and duct area. This will kill about all bacteria, but some mold spores will still make it through, so you still need a mechanical pre or post filter to do a complete job. You can not, however, use a single small tube to disinfect a room's air. Light intensity falls off at an exponential rate the further you get from the bulb, so it will only "clean" the air that is relatively close by. The forced air UV filters have to run the air through a confined space to that there is enough light intensity to do a reasonable job.



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Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Alcohol 80% better than 96%!! [Re: Shroomzilla]
    #270734 - 03/13/01 05:51 AM (20 years, 7 months ago)

I know that QT ammonium is also used in the food industrie to desinfect, but I know there are better desinfectants.
I will take my schoolbooks and try to find some other desinfectants because there are more!!
Bleach can also enter plastics so you'll have to clean it afterwards with sterile water.
And shroomzilla is right about the UV-light they also use it in microbiological laboratories where I work!!
Peace and happiness


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OfflineKast
old hand
Registered: 12/01/00
Posts: 222
Last seen: 16 years, 6 months
Re: Alcohol 80% better than 96%!! [Re: Shroomzilla]
    #270806 - 03/13/01 11:01 AM (20 years, 7 months ago)

Shroomzilla: And you say that GE sells these UV bulbs? Where could I find one, just a regular hardware store or would I need to go through a microbiology supply company?

Paddo: Did you build your flowhood yourself? I've given thought to doing this, but not so much out of necessity as out of boredom.



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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: Alcohol 80% better than 96%!! [Re: paddoholland]
    #272604 - 03/15/01 08:38 PM (20 years, 7 months ago)

bump



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"habit is the ballast that chains the dog to its vomit" - s beckett


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InvisibleSoup
enthusiast
Registered: 12/13/98
Posts: 29
Re: Alcohol 80% better than 96%!! [Re: rebelmoon]
    #272638 - 03/15/01 09:16 PM (20 years, 7 months ago)

Paddoholland,

I can understand why 70 or 80% would kill mo's better when wiped or dunked in it but who cares if it is being burned? Flame is flame. I (or at least I used to) dunk, burn off and keep on flaming til red hot. Then for transfers directly after I dunk and just burn off. I spray with lysol 5 minutes before entering my bathroom which I never sterilize anymore and I have virtually no contamination with agar or grain. Something must be sterilizing my scalpel and keeping it that way and it is rarely a pressure cooker.



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OfflineShroomzilla
enthusiast

Registered: 02/04/01
Posts: 177
Loc: Texas, home of the longho...
Last seen: 19 years, 7 months
Re: Alcohol 80% better than 96%!! [Re: Soup]
    #272757 - 03/15/01 11:40 PM (20 years, 7 months ago)

Its all a matter of using what where: You can flame sterilize your scalpel or inoculating loop...but not your counter top or floor. The original post was about what concentration of alcohol works best, the real answer is "none of them" as they take far too long a contact time to be effective for most uses.

"Ethyl alcohol (ethanol) and isopropyl alcohol are frequently used for chemical disinfection. They are mainly used as skin antiseptics and act by "denaturing" or altering the molecular structure of bacterial proteins, destroying the cell. Alcohols kill vegetative forms of bacteria but have no action on spores or viruses." from Sterilization: Methods and Control, Butterworth & Co, 1996.

Alcohol, as noted above, is fine for killing active living bacteria, but is ineffective against spores. While bacterial contamination does occur from time to time, the #1 problem is with mold. Flame sterilizing your needles, scalpels, etc, is the best way, but you have to use other type of effective disinfection for everything else



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