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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Speeker]
    #9564249 -

While browsing recent issues of Mycological Research, I stumbled upon this and thought of you. I do not have access to the full text of the paper, but if you have access to a library they should be able to get you a reprint or get this issue via interlibrary loan.

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7XMR-4SWWTBY-1&_user=2429682&_coverDate=11%2F30%2F2008&_rdoc=10&_fmt=high&_orig=browse&_srch=doc-info(%23toc%2329677%232008%23998879988%23701008%23FLA%23display%23Volume)&_cdi=29677&_sort=d&_docanchor=&_ct=14&_acct=C000057245&_version=1&_urlVersion=0&_userid=2429682&md5=46031daf68142fed23d8b5aade9bff04

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
    #9570950 -

Hey Denger.. good to hear from you bro.  I am actually doing research on the subjuct right now in my campus library.. thanks for the link man bout to check it out now.. will get back soon.

  I am beginning the first round of experiments in two weeks, it would be great if anyone had any advice or knew of any places where i could possibly gain access to a larger autoclave.. or perhaps a good source on used autoclaves, even building instructions.. dont worry i wont blow myself up, my partner is a mechanical engineer :smile:


--------------------

Koopa Out
OfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #9571081 -

Building autoclaves is not the best way to spend your time/money. You will end up spending more $$ on materials and parts then on buying a brand new one.
How big do you need it? AA pressure cookers go all the way to 41qt size, thats twice the size of a standard issue medical autoclave.
Check ebay if you need bigger. Your best bet for bigger ones is a "retort" made for canning industry. Again, ebay, or canning industrial supply place.

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
    #9748735 -

I am generally interested in this subject. I would like to learn how to grow truffles hydroponically. Let me know how your experiments turn out.

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Amanita Sporosa]
    #9750268 -

Hey everybody, sorry for leaving this thread hanging for awhile for those that where interested. I am starting to actually make some progress now. Deng, thanks alot man for the interest and the wisdom you have provided.

Amanita, i suggest you read all the links ive provided on here and if you have access to a university, I strongly suggest that you use their computers to read as many scientific journal articles on any of the tuber genus and ecto and endomycorrhizal studies, research and patents you can find.

It is a subject very clouded in secrecy and a general lack of solid knowledge, which I have come to believe that to some extent is very purposefull.

The only advice I can give you at the moment is that I have came to learn that most of the methods I stated above are more suited towards acadamia and research and are impractical for field use and large scale production.  I will update this thread after my first round of experiments are done, hopefully within the following month.


--------------------

Koopa Out
OfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #10260128 -

So im about to begin work on site preparation for beginning my truffle orchard. Ive received close to 200 acres of farm and field land, along with all the major equipment I will need to cultivate it. Cant believe everything is falling into place so easily for once in my life :tongue:

  Ive been keeping correspondence with both Tom Michaels (of Tennessee Truffles fame) and Gerard Chevalier (THE truffle expert from France) who have both provided invaluable help. I will be starting a new thread soon which will detail the growth and progress of my opperation, but for now I will lay out some general info to give everybody something to chew on and to also help me get this shit out of my brain lol been obsessively researching all things Truffle related for some time now.. Lord knows the girlfriend is Fn tired of even hearing the word spoken God bless er' lol.

Next week, after my soul raping final exams are over with, I will begin construction of two large greenhouses in which Oak and Filbert saplings will be sprouted and inoculated in. After finishing construction, I will begin land preperation which includes tilling the land, and spreading approximately 1 ton of lime per acre to achieve the proper PH. Thankfully one of my friends owns a Grading company and has very cheap access to bulk amounts of lime ($50 per 1/2ton!) and also a spreader that holds 1/2 ton per load. Next, I will begin fertilizing the land, which ingredients will remain somewhat secret but you can get a pretty good idea by studying patents and research papers on the subject at university libraries:grin:.

Site selection for truffle orchard development is also very important.. ideally you should look for land that is similar to any that would be ideal for farming.. large open areas that would be easy to irrigate and most importantly, land that has not had prior tree growth recently. This helps limit the amount of domestic mycorhizal competition for the truffle to overcome. Tree selection is also very important. In the primary testing and selection stage, one should test several species prevalent in truffle preduction in France with native candidates (usually those of the Oak variety). Excellent info on this subject can be found in Tom Micheals research thesis on Invitro Truffle Growth.  Once ideal host candidates are determined through random statistical analysis on growth vigor and mycorhizal abundance you should be feeling like a certified bad ass.

Next I will describe a simple method for developing the mycorhizal symbiosis between the tree and the truffle fungis. First, oak seeds must be surface sterilized with a .1% solution of silver nitrate for 20 minutes and then rinsed well with sterile water to remove all remaining traces of the substance. Next one must acquire fresh or frozen truffles allowing them to thaw if frozen then place in a jar or similar container filled with water allowing the truffle to degredate slightly. After degredation, dissolve 1 kg of dextrose or similar simple sugar per 20 liters of truffle suspension, creating what is called a truffle slurry. Some truffliers (growers of the truffle, duh :wink:) like to add snail enzymes to the slurry claiming beneficial effects on mycorhization rates.

Next, you must prepare the potting soil. Each trufflier has their own secret potting mix, but a simple example is 20% loess soil, 30% organic material, 50% vermiculite, dolemite to raise PH and slow release Osmocoat fertilizer. The soil mix should then be pastuerized to remove detrimental microorganisms.
Mix the truffle slurry into the potting soil mix (again, many ideal soil preperations can be found in many truffle patents and research papers) once the tree outgrows its pot after a few months, remove it and gently wash the roots with water to view its inoculation  process, noting growth rates and statistical differences between other saplings. Next, dip the roots in a second mix of truffle slurry before adding it to a larger pot. This should all be done in an enclosed greenhouse or a lab/controlled grow room to minimize contam rates and controlled environmental factors to reduce sapling deaths and increase inoculation rates. After saplings have reached a year or two in age, they may be transferred to the previously prepared land to hopefully live long and happy truffle bearing years, preferably while you sip lemonade and laugh at wall street douche bags.

After 4-10 years you will hopefully begin to notice the ground to begin to knot around the trees, followed by burn circles causing the death of the grass around the trees. You should begin to notice a sweet pungent odor in the air, next tell any low life, favor asking, money borrowing brother in law to go frack a swine flu infested Panama hooker... while you and Chittlins (your prize winning truffle finding pig) cruise town in your drop-top 67 Vette! :evil:

Appologies for all the spelling/gramatical errors.. 3am and taking a study break from finals, figured I would share a cliff notes version of my future truffle orchard. Hope everyone enjoys the read, and keep a look out for the picture tutorial ill be making in the next couple weeks! Wish me luck, ill sure as hell need it!


--------------------

Koopa Out
OfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #10260845 -

Hey Koopa_troopa,

very interesting, thanks for sharing! It seems you got pretty far in a short time. Now I'm looking forward to reading more and seeing some images.

Carsten

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Mycelio]
    #10264665 -

Thanks alot Mycello, Ive been literally obsessed with the topic since the initial post.

I hope to eventually start my own company supplying inoculated saplings to other truffle orchard startups once a get a few years of hands on practice inoc'n several thousand trees :grin:.. the NC Agricultural Foundation estimates that within the next 10 yrs, the amount of truffle orchards in this state alone will increase some 5000%. And with average orders for saplings per orchard between 600 and 5000, and the avg price per sapling being between $30-$80.. there should be some pretty nice potential for profit.

The NC Agricultural Foundation also estimates that a properly managed truffle orchard could net around $30,000 per acre once they begin bearing truffles, and possibly for 20-40 years!!! So with 250 acres of prime truffle growing land at my disposal, and between two of my best friends becoming partners with me, who just happen to own all the possible farming and grading equipment I could ever possibly need.. Im giddier than a Mormon virgin in Vegas :tongue:

Its incredible how after years of life taking a shit on you, how suddenly one random day you can turn around and make life your bitch for a change haha.. But seriously I couldnt be happier with how everything is just falling into place so seamlessly.. ill be damned if I let this fish off the hook! All thats standing in my way now are the next two weeks of hellaciously mind rapping final exams..lol wish me luck


--------------------

Koopa Out
OfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #10265463 -

That sounds great koopa .. I need to order me some sapplings. I've got some land i'd love to put some of those trees on.

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #24136321 -

koopa_troopa said:
So im about to begin work on site preparation for beginning my truffle orchard. Ive received close to 200 acres of farm and field land, along with all the major equipment I will need to cultivate it. Cant believe everything is falling into place so easily for once in my life :tongue:

  Ive been keeping correspondence with both Tom Michaels (of Tennessee Truffles fame) and Gerard Chevalier (THE truffle expert from France) who have both provided invaluable help. I will be starting a new thread soon which will detail the growth and progress of my opperation, but for now I will lay out some general info to give everybody something to chew on and to also help me get this shit out of my brain lol been obsessively researching all things Truffle related for some time now.. Lord knows the girlfriend is Fn tired of even hearing the word spoken God bless er' lol.

Next week, after my soul raping final exams are over with, I will begin construction of two large greenhouses in which Oak and Filbert saplings will be sprouted and inoculated in. After finishing construction, I will begin land preperation which includes tilling the land, and spreading approximately 1 ton of lime per acre to achieve the proper PH. Thankfully one of my friends owns a Grading company and has very cheap access to bulk amounts of lime ($50 per 1/2ton!) and also a spreader that holds 1/2 ton per load. Next, I will begin fertilizing the land, which ingredients will remain somewhat secret but you can get a pretty good idea by studying patents and research papers on the subject at university libraries:grin:.

Site selection for truffle orchard development is also very important.. ideally you should look for land that is similar to any that would be ideal for farming.. large open areas that would be easy to irrigate and most importantly, land that has not had prior tree growth recently. This helps limit the amount of domestic mycorhizal competition for the truffle to overcome. Tree selection is also very important. In the primary testing and selection stage, one should test several species prevalent in truffle preduction in France with native candidates (usually those of the Oak variety). Excellent info on this subject can be found in Tom Micheals research thesis on Invitro Truffle Growth.  Once ideal host candidates are determined through random statistical analysis on growth vigor and mycorhizal abundance you should be feeling like a certified bad ass.

Next I will describe a simple method for developing the mycorhizal symbiosis between the tree and the truffle fungis. First, oak seeds must be surface sterilized with a .1% solution of silver nitrate for 20 minutes and then rinsed well with sterile water to remove all remaining traces of the substance. Next one must acquire fresh or frozen truffles allowing them to thaw if frozen then place in a jar or similar container filled with water allowing the truffle to degredate slightly. After degredation, dissolve 1 kg of dextrose or similar simple sugar per 20 liters of truffle suspension, creating what is called a truffle slurry. Some truffliers (growers of the truffle, duh :wink:) like to add snail enzymes to the slurry claiming beneficial effects on mycorhization rates.

Next, you must prepare the potting soil. Each trufflier has their own secret potting mix, but a simple example is 20% loess soil, 30% organic material, 50% vermiculite, dolemite to raise PH and slow release Osmocoat fertilizer. The soil mix should then be pastuerized to remove detrimental microorganisms.
Mix the truffle slurry into the potting soil mix (again, many ideal soil preperations can be found in many truffle patents and research papers) once the tree outgrows its pot after a few months, remove it and gently wash the roots with water to view its inoculation  process, noting growth rates and statistical differences between other saplings. Next, dip the roots in a second mix of truffle slurry before adding it to a larger pot. This should all be done in an enclosed greenhouse or a lab/controlled grow room to minimize contam rates and controlled environmental factors to reduce sapling deaths and increase inoculation rates. After saplings have reached a year or two in age, they may be transferred to the previously prepared land to hopefully live long and happy truffle bearing years, preferably while you sip lemonade and laugh at wall street douche bags.

After 4-10 years you will hopefully begin to notice the ground to begin to knot around the trees, followed by burn circles causing the death of the grass around the trees. You should begin to notice a sweet pungent odor in the air, next tell any low life, favor asking, money borrowing brother in law to go frack a swine flu infested Panama hooker... while you and Chittlins (your prize winning truffle finding pig) cruise town in your drop-top 67 Vette! :evil:

Appologies for all the spelling/gramatical errors.. 3am and taking a study break from finals, figured I would share a cliff notes version of my future truffle orchard. Hope everyone enjoys the read, and keep a look out for the picture tutorial ill be making in the next couple weeks! Wish me luck, ill sure as hell need it!



Thank you so much sharing this information. How did your enterprise work out? I am asking because I want to plant my own orchard and inoculate my own seedlings because at 24 a pop it would just be ridiculously expensive not to so. Please post an update or share any lessons you have learnt. Thank you so much.

Edited by Farmergirl (03/04/17 02:04 PM)

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: SOUTHERN]
    #26183209 -

SOUTHERN said:
That sounds great koopa .. I need to order me some sapplings. I've got some land i'd love to put some of those trees on.



Sorry

Edited by Javichunte (09/12/19 03:14 AM)

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
    #26183217 -

koopa_troopa said:
So im about to begin work on site preparation for beginning my truffle orchard. Ive received close to 200 acres of farm and field land, along with all the major equipment I will need to cultivate it. Cant believe everything is falling into place so easily for once in my life :tongue:

  Ive been keeping correspondence with both Tom Michaels (of Tennessee Truffles fame) and Gerard Chevalier (THE truffle expert from France) who have both provided invaluable help. I will be starting a new thread soon which will detail the growth and progress of my opperation, but for now I will lay out some general info to give everybody something to chew on and to also help me get this shit out of my brain lol been obsessively researching all things Truffle related for some time now.. Lord knows the girlfriend is Fn tired of even hearing the word spoken God bless er' lol.

Next week, after my soul raping final exams are over with, I will begin construction of two large greenhouses in which Oak and Filbert saplings will be sprouted and inoculated in. After finishing construction, I will begin land preperation which includes tilling the land, and spreading approximately 1 ton of lime per acre to achieve the proper PH. Thankfully one of my friends owns a Grading company and has very cheap access to bulk amounts of lime ($50 per 1/2ton!) and also a spreader that holds 1/2 ton per load. Next, I will begin fertilizing the land, which ingredients will remain somewhat secret but you can get a pretty good idea by studying patents and research papers on the subject at university libraries:grin:.

Site selection for truffle orchard development is also very important.. ideally you should look for land that is similar to any that would be ideal for farming.. large open areas that would be easy to irrigate and most importantly, land that has not had prior tree growth recently. This helps limit the amount of domestic mycorhizal competition for the truffle to overcome. Tree selection is also very important. In the primary testing and selection stage, one should test several species prevalent in truffle preduction in France with native candidates (usually those of the Oak variety). Excellent info on this subject can be found in Tom Micheals research thesis on Invitro Truffle Growth.  Once ideal host candidates are determined through random statistical analysis on growth vigor and mycorhizal abundance you should be feeling like a certified bad ass.

Next I will describe a simple method for developing the mycorhizal symbiosis between the tree and the truffle fungis. First, oak seeds must be surface sterilized with a .1% solution of silver nitrate for 20 minutes and then rinsed well with sterile water to remove all remaining traces of the substance. Next one must acquire fresh or frozen truffles allowing them to thaw if frozen then place in a jar or similar container filled with water allowing the truffle to degredate slightly. After degredation, dissolve 1 kg of dextrose or similar simple sugar per 20 liters of truffle suspension, creating what is called a truffle slurry. Some truffliers (growers of the truffle, duh :wink:) like to add snail enzymes to the slurry claiming beneficial effects on mycorhization rates.

Next, you must prepare the potting soil. Each trufflier has their own secret potting mix, but a simple example is 20% loess soil, 30% organic material, 50% vermiculite, dolemite to raise PH and slow release Osmocoat fertilizer. The soil mix should then be pastuerized to remove detrimental microorganisms.
Mix the truffle slurry into the potting soil mix (again, many ideal soil preperations can be found in many truffle patents and research papers) once the tree outgrows its pot after a few months, remove it and gently wash the roots with water to view its inoculation  process, noting growth rates and statistical differences between other saplings. Next, dip the roots in a second mix of truffle slurry before adding it to a larger pot. This should all be done in an enclosed greenhouse or a lab/controlled grow room to minimize contam rates and controlled environmental factors to reduce sapling deaths and increase inoculation rates. After saplings have reached a year or two in age, they may be transferred to the previously prepared land to hopefully live long and happy truffle bearing years, preferably while you sip lemonade and laugh at wall street douche bags.

After 4-10 years you will hopefully begin to notice the ground to begin to knot around the trees, followed by burn circles causing the death of the grass around the trees. You should begin to notice a sweet pungent odor in the air, next tell any low life, favor asking, money borrowing brother in law to go frack a swine flu infested Panama hooker... while you and Chittlins (your prize winning truffle finding pig) cruise town in your drop-top 67 Vette! :evil:

Appologies for all the spelling/gramatical errors.. 3am and taking a study break from finals, figured I would share a cliff notes version of my future truffle orchard. Hope everyone enjoys the read, and keep a look out for the picture tutorial ill be making in the next couple weeks! Wish me luck, ill sure as hell need it!



Hello, how was everything? Please, we need your wisdom, help us

Edited by Javichunte (09/12/19 03:24 AM)

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