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Invisiblemycofile
Pooh-Bah
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Mutations, *PLEASE DONT FLAME ME*
    #86329 - 06/19/00 02:32 AM (16 years, 5 months ago)

OK, no flames as you asked. But I think this is a really irresponsible post. You know damn well how many inexperienced/young people come here and will likely see this. A statement such as:

"Colchicine - The common mushroom mutagen. Supposedly used br Mr G to create most of his strains. Fairlt easy to work with. One needs to make a colchicine agar, and streak some spores or mycelium on it, than transfer a bit of healthy mycelium into a celan agar plate(no colchicine) and try an fruit the new strain. Causes mutations in unpredictable places in the DNA chain."

s dangerous and totally innacurate. First of all, by saying that colchicine is commonly used and can be used easily, you are risking peoples health and possibly lives by making it seem common and safe. COLCHICINE IS AN EXTREMELY DANGEROUS CHEMICAL!!! It is not easy to work with. You can't just toss it around. If you do, you will hurt yourself and likely others around you. Any mention of colchicine without warnings of it's dangers is extremely irresposible. Secondly, it's not the common mushroom mutagen. Who else do you know that's worked with it? Is it more common than random mutation? UV mutation? I certainly doubt it, because it is much more dangerous and not many people work with it.

Also, it doesn't cause mutations in unpredictable places in the DNA chain at all. Have you done any research here? I know for a fact that the effects have been discussed rather thouroughly here b4. Colchicine doubles the number of chromosomes in actively reproducing cells(that is the ones that survive!). That's not random, and it's not within the DNA chain. It is called polyploidy and there are safer ways to achieve it. It is extremely predictable and simply doubles the chromosomes.

Also, no mention not to eat the mutated fruit, esp from colchicine!!!??? By no means should the first generation ever be consumed, and some have even expressed concerns about any generations being edible. Leaving out this type of information can drastically alter ones life forever.

Be aware that working with colchicine is extremely dangerous. I wish I had listened to Dr. Lindeman long ago. This post is the exact type of thing he was afraid would happen. I disagreed assuming that people would do their research before risking their health or the health of others, but I guess not.

I also question any information that is contained in a post that lacks such relevant information. You said no flames, so I think this is just an informed objection. Don't encourage experimentation with things either you don't fully understand the implications of, at least give a warning, or in other words kids:
DON'T TRY THIS AT HOME!!!!

Hey doc, you wanna switch sides now that I took up your old argument? :wink:

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)

Visit The Donkey! just behave



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Mutations, *PLEASE DONT FLAME ME*
    #86331 - 06/19/00 03:09 AM (16 years, 5 months ago)

OK, I guess that came out a little rougher than it was meant. In the sake of information rather than dis-information, this is an excellent thread I dug up.
http://www.shroomery.org/ubbnoncgi/Forum4/HTML/000317.html


Let's just say that I've been working with this one for a while and though I'll plead the fifth on any further questions, I will say that the stuff scares me more by the day.

One more rant. Do a little research on this subject, and you will quickly learn how full of shit much info on the web is. Especially on BBs where any idot or genius can post as opposed to articles written by a genius and archived by idiots. People, if you aren't smart enough to understand what you are doing better than the people that talk about this kind of thing on-line, don't do it!
Enjoy.


------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)

Visit The Donkey! just behave

[This message has been edited by mycofile (edited June 19, 2000).]



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: Mutations, *PLEASE DONT FLAME ME*
    #86332 - 06/19/00 09:00 AM (16 years, 5 months ago)

Ok, mycofile,
about colchicine, I was talking from my experience with it, and the mutations you've mentioned dont happen all the time, though it mostly replicates the number of chromosomes (which in turn affects the DNA chain in the next generation). I did a few experiments with it n the University, on mushrooms and on germs. I am very sorry I did not mention the dangers about the use of it. I assumed ppl with no experience wont even read this. I am sorry. If you wish I can delete it, and we could descuss in private.
The UV light is indeed dangereous but as long as you do it in a closed non-transparent container it'll be fine.
If you think about it, colchicine is the "common" mushroom mutagen, since no one barely mutates shrooms and this is the most used one.

trade_omlet

------------------
How do you like your eggs?
scrambled? OMLET?



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Anonymous

Re: Mutations, *PLEASE DONT FLAME ME*
    #86333 - 06/19/00 09:03 AM (16 years, 5 months ago)

Wow!
My name up in lights!
My 15 minutes of fame.
Thank you Mycofile!!!!!!
I appreciate your opinion!
This is best left up to the experts, but that is like hearing "No honey, you can't have that cookie, it is bad for you."
Now you want that damn cookie worse than ever.
If you do this, you do so at your own risk!!!
Read about the stuff before experimenting.
I do not recommend it.
Don't do it!
But post your results here!

Now Mycofile, I want you to e-mail me.
madmycologist@webtv.net
( message edited on behalf of Trade omlett. He asked me not to post certain personal info. I don't like him right now, but I will respect him and honor his request.)

Or call me.
Hell i'm rich- I can call you if you want.
Let me know.
Thank you again Mycofile!
You have just made my day!
God bless you.
Well, the drugs are starting to wear off, i'd better go.
God bless you all!
To trade omlett, Shalom my friend.
G'night.

------------------

What little I know....

[This message has been edited by Dr. Charles Lindeman (edited June 19, 2000).]



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Anonymous

Re: Mutations, *PLEASE DONT FLAME ME*
    #86334 - 06/19/00 09:42 AM (16 years, 5 months ago)

Sorry, double post.
I'm afraid I am tripping very hard right now. Time to smoke my breakfast!

[This message has been edited by Dr. Charles Lindeman (edited June 19, 2000).]



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Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Mutations, *PLEASE DONT FLAME ME*
    #86335 - 06/20/00 04:59 AM (16 years, 5 months ago)

No need to delete, as is obvious, this stuff has been discussed before. If you would like to discuss privately, my email is listed above.

Dr., you've got mail (please don't try to smoke it :wink:

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)

Visit The Donkey! just behave



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Post Extras: Print Post  Remind Me! Notify Moderator
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