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Offlinetchyted
miestro
Registered: 09/03/01
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Loc: WA near seattle
Last seen: 2 years, 3 months
use of x rays to induce random mutations
    #1559949 - 05/19/03 02:12 PM (13 years, 7 months ago)

WARNING, X-RAYS CAN CAUSE SERIOUS IRREPARABLE DAMAGE TO HUMAN TISSUE AS WELL AS CAUSE CANCER, ETC.


i recall experiments done with seeds and an old vacume tube tv that i did in high school. anyway, i got some weird marigolds out of the mess.

since old tv's are plentifull, and since the dangerous radiation is mostly confined to the inside of the cabinet, i am proposing to expose a spore print by placing it in close proximity to one of the vacume tubes inside.

i am seeking input from others who may have done similar experiments, other than PF's gama exposure. I seek only safe proceedures.



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OfflineZeppelin
I'm Gonna BurnOne Down.

Registered: 04/08/03
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Re: use of x rays to induce random mutations [Re: tchyted]
    #1561991 - 05/20/03 01:31 AM (13 years, 7 months ago)

Never attempted anything like that before... never had access to equipment nor did I have the knowledge. Good luck and keep the progress posted.


--------------------
I'm afraid of losing my obscurity.
Genuineness only thrives in the dark.

Like celery. ~Aldous Huxley


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OfflineNoLogos
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Registered: 02/28/03
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Re: use of x rays to induce random mutations [Re: Zeppelin]
    #1562402 - 05/20/03 07:52 AM (13 years, 7 months ago)

I *think* you will have better luck if you hydrate the spores first. I know that is the case with plant seeds--when they are dry, they are much more resistant to environmental insults. Why don't you put some seeds in there too? Look for a nice leaf mutation for me!


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OfflineNeonBlack
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Re: use of x rays to induce random mutations [Re: tchyted]
    #1562937 - 05/20/03 02:48 PM (13 years, 7 months ago)

I think that it might be a little easier and safer to use a short wave uv light. But, you would have to do a LOT of plates to find something that would even fruit let alone something with a favorable mutation. It would be an interesting experiment though.


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OfflineGWAR
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Re: use of x rays to induce random mutations [Re: NeonBlack]
    #1567632 - 05/22/03 12:20 AM (13 years, 7 months ago)

try it with herb, maybe u can get buds from plants taht dont look like bud plants... or maybe if u put spores in with weed seeds u will get crazy buds that make u trip!


--------------------

"Freedom to all the people... Brave, true and strong... Freedom to all the people... Unless I think you're wrong!!!"


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OfflineRadioActiveSlug
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Re: use of x rays to induce random mutations [Re: GWAR]
    #1567747 - 05/22/03 12:59 AM (13 years, 7 months ago)

why would you want to mutate something you'd put into your body? this is not a comic book, or the ninja turtles cartoonn, mutations are most often a result of cancerous cells run amuck, not something you want to forcefully cause in a living thing.

you guys are scareing me with this mad scientist shit.


--------------------
"Holding on to anger is like grasping a hot coal with the intent of throwing it at someone else; you are the one getting burned." -Buddha
www.impeach-bush-now.org


Edited by RadioActiveSlug (05/22/03 01:00 AM)


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OfflineBladeLSD
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Registered: 08/25/00
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Re: use of x rays to induce random mutations [Re: RadioActiveSlug]
    #1567790 - 05/22/03 01:11 AM (13 years, 7 months ago)

Fanaticus experimented with Gamma radiation to sterilize PF-Jars, How about exposing the mycellium to radiation. I dont know about eating the results...Sounds interesting though.


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


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Anonymous

Re: use of x rays to induce random mutations [Re: RadioActiveSlug]
    #1567795 - 05/22/03 01:12 AM (13 years, 7 months ago)

No logos: Hydrating the spores will not have any effect. It is not "luck" that dictates mutations.

You're exposing them to X-RAY radiation. Their DNA is going to get pierced by X-RAYs regardless to whether they are hydrated or not.

Do you have any factual evidence of what you state?


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Invisiblemicro
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Re: use of x rays to induce random mutations [Re: daba]
    #1571098 - 05/23/03 02:41 AM (13 years, 7 months ago)

X-rays will cause random mutations, but I don't think a T.V. tube will be strong enough to do what you need. You have to keep in mind that these mutations are random, and there are a lot of genes in the mushroom's genome, so a lot more work would need to be done if you are dealing with a small amount of random deletions, etc. Feel free to try, though -- just keep in mind that sucker's around 14,000 volts inside which can shock you from ~ 1 1/2 cm away.

You could always use a high frequency UV lamp, instead (i.e. UV-C.) The TV could possibly work, though, and there is no reason not to try if you want to; just don't get shocked!

Just my opinion,

--
Micro


--------------------
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Invisiblepoke smot!
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Registered: 01/08/03
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Re: use of x rays to induce random mutations *DELETED* [Re: micro]
    #1571734 - 05/23/03 10:51 AM (13 years, 7 months ago)

Post deleted by poke smot!

Reason for deletion: x



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OfflineRaedon
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Registered: 04/07/03
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Re: use of x rays to induce random mutations [Re: poke smot!]
    #1573672 - 05/24/03 01:22 AM (13 years, 7 months ago)

there is a good chance that even if you were able to mutate a gene it would have no visable effect. It would be more productive to look into gene insertion then random chance. Maybe a glow in the dark shroom? Or shrooms that produced N,N,DMT; Or hell, LSD..

EDIT: wait, LSD doesn't occur in nature so no gene for it's production.


Edited by Raedon (05/24/03 01:24 AM)


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InvisibleAlkaloids
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Re: use of x rays to induce random mutations [Re: tchyted]
    #1574628 - 05/24/03 04:02 PM (13 years, 7 months ago)

I agree that doing insertions would be the most effective way to get results, but seeing as how that requires a bit more equipment and know-how (just a bit :smile:  )    I say keep trying out what you are doing.  Most mutations that express themselves on a macroscopic level tend to be deleterious, but You might just come up with something better.  Might take a long time, but what else is life good for if not experimenting?   


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Invisiblemicro
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Re: use of x rays to induce random mutations [Re: Alkaloids]
    #1574854 - 05/24/03 06:32 PM (13 years, 7 months ago)

I would think it would be relatively easy to make it produce DMT, or LSA. Just time consuming and you'd have to pay for assays, etc., etc.

They are all made on the same metabolic pathway.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (05/24/03 07:01 PM)


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Offlinejehst
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Registered: 05/18/03
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Re: use of x rays to induce random mutations [Re: micro]
    #1574951 - 05/24/03 07:40 PM (13 years, 7 months ago)

shrooms aren't like little bacteria..their have a pretty big genome. random mutations is going to get you nowhere unless you are extremely lucky, IMO.

imagine if someone found the gene that controls the amound of psilocybin in the shrooms and made the shrooms produce like 50x more of it..pwnage.


--------------------
All I have in this world are my balls and my word, and I don't break 'em for no one. You understand me?


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Invisiblemicro
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Re: use of x rays to induce random mutations [Re: jehst]
    #1575090 - 05/24/03 09:10 PM (13 years, 7 months ago)

or something that inhibits its downregultion :wink:

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisiblePrisoner#1M
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Re: use of x rays to induce random mutations [Re: BladeLSD]
    #1575340 - 05/24/03 11:23 PM (13 years, 7 months ago)

Quote:

Fanaticus experimented with Gamma radiation to sterilize PF-Jars, How about exposing the mycellium to radiation. I dont know about eating the results...Sounds interesting though.




Actualy I believe that was Yachaj...but it could have been both...

P#1


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InvisiblePrisoner#1M
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Re: use of x rays to induce random mutations [Re: Prisoner#1]
    #1575355 - 05/24/03 11:33 PM (13 years, 7 months ago)

X-RAY MACHINE....

....

A friend of mine sells X-Ray equipment, he rebuilds and repairs and ships worldwide...currently I have a cambodian on glass 3"x3"...now...I'm not sure what it will take to talk him into zapping them but it's a true x-radiation and the strength can be as high as you want....I'll call him on tuesday and see if he can do it...he's done my ankle with about 9 different vies to find out that its broken....Dr. at the emergency room was wrong...I may wanna put this up for bid.....or make a good trade....especialy any spores mounted on slides....just broke out the microscope and ordered a new camera a couple of new objectives and a micrometer....taxonomy time....

I'll let you guys know on tuesday

P#1


Edited by Prisoner#1 (05/24/03 11:38 PM)


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OfflineMindzpore
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Re: use of x rays to induce random mutations [Re: Prisoner#1]
    #4375539 - 07/06/05 01:43 PM (11 years, 6 months ago)

this is crazy talk this is.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
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Offlinescatmanrav
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Re: use of x rays to induce random mutations [Re: Mindzpore]
    #4375743 - 07/06/05 02:52 PM (11 years, 6 months ago)

>i am seeking input from others who may have done similar experiments, other than PF's gama exposure. I seek only safe proceedures.

PF just grew his shrooms with a blacklight...how isnt that safe? Of course he just ended up with sporeless shroomies...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleTien
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Re: use of x rays to induce random mutations [Re: scatmanrav]
    #4375835 - 07/06/05 03:23 PM (11 years, 6 months ago)

I remember before 9/11 you could buy smaller chunks of RAW Uranium on Ebay, natural Uranium produces Alpha, Beta, and Gamma radiation.

I'm sure if you place a chunk of U-238 (natural uranium) beside a print or a culture, you'd get something.

Pluto


Edited by Plutonium (07/06/05 03:23 PM)


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