monstermitch said:

sometimes yes, sometimes no.

not every fruit is born of a single strain.
strains that have not 'mated' are capable of collaborating to
produce a single fruit or cluster of fruits.

on agar, with clone tissue, often you can see distinct separate
sectors grow and from from the biopsied tissue. 
so as I see it (and who knows if I even have a clue what I'm
talking about...) if you're lucky you can clone an isolate, but most
of the time you'll just be reducing the strains to two of them or so.
fruits found in the wild have a greater chance of being born of
a greater number of strains.  so you would have an even smaller
chance of cloning an isolate from the wild.  home fruits would be
your best bet.

so after cloning the fruit to agar, further isolate from there, and
grow out each sector on it's own and fruit side by side.  keep the
one you like the best and toss the other one.

(if I even know what I'm talking about... )

lately I cloned a PE fruit that heavily sporulated...
the cloned culture grows produced about 1 in 200 fruits that
produced spores....
so it's not always as easy as it might sound.
time to go into further isolation....

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