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Offlinewutang
fungi
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Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
how cloning works
    #7544324 - 10/21/07 07:52 PM (16 years, 3 months ago)

well i made a few successful agar clones.
great.  easy.
but WHO EXACTLY KNOWS how it works??
i got a monster pin thats going to be a monster shroom,
but im so confused!!!!! :frown:
does it mean that once you transfer a wedge to spawn and inoculate then fruit.
will all the shrooms that pop up be like just like  the one i transfered to agar?
same size? ect. when i hear cloning thats what i think of.

btw i searched articles on agar but only found how to teks


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:peyotespectrum:    :sanpedro: :rainbowdrink: :tripping:


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Offlinesyntroniks
Stranger Danger
Registered: 10/18/07
Posts: 76
Loc: Assen, Holland
Last seen: 16 years, 1 month
Re: how cloning works [Re: wutang]
    #7544373 - 10/21/07 08:01 PM (16 years, 3 months ago)

I think that is generally how it is, but I wouldn't think they would all be exactly the same. Now if you cloned the shorted shroom then fruited and cloned that one's shortest and so on, you'd get short shrooms I know that


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Invisibleshroomerite
Apprentice


Registered: 06/09/06
Posts: 513
Re: how cloning works [Re: syntroniks]
    #7544393 - 10/21/07 08:06 PM (16 years, 3 months ago)

Yeah, basicly you get a sort of "substrain" isolate the has common fruiting capabilities. The goal is to isolate a good fruitng strain that way you get consistant fruits and potency along with more even pinsets.


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  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisiblemonstermitch
Growing in Bags Doesn't Work


Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: how cloning works [Re: shroomerite]
    #7544526 - 10/21/07 08:37 PM (16 years, 3 months ago)

sometimes yes, sometimes no.

not every fruit is born of a single strain.
strains that have not 'mated' are capable of collaborating to
produce a single fruit or cluster of fruits.

on agar, with clone tissue, often you can see distinct separate
sectors grow and from from the biopsied tissue. 
so as I see it (and who knows if I even have a clue what I'm
talking about...) if you're lucky you can clone an isolate, but most
of the time you'll just be reducing the strains to two of them or so.
fruits found in the wild have a greater chance of being born of
a greater number of strains.  so you would have an even smaller
chance of cloning an isolate from the wild.  home fruits would be
your best bet.

so after cloning the fruit to agar, further isolate from there, and
grow out each sector on it's own and fruit side by side.  keep the
one you like the best and toss the other one.

(if I even know what I'm talking about... :naughty: )

lately I cloned a PE fruit that heavily sporulated...
the cloned culture grows produced about 1 in 200 fruits that
produced spores....
so it's not always as easy as it might sound.
time to go into further isolation....


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Offlinewutang
fungi
 User Gallery


Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
Re: how cloning works [Re: monstermitch]
    #7544621 - 10/21/07 09:02 PM (16 years, 3 months ago)

very helpful advice mitch
and very interesting how a cloned PE that sporulated still didnt produce any that did.


--------------------
:peyotespectrum:    :sanpedro: :rainbowdrink: :tripping:


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Offlinesomebody041
bud tester
Male User Gallery

Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
Re: how cloning works [Re: monstermitch]
    #7544853 - 10/21/07 09:59 PM (16 years, 3 months ago)

Quote:

monstermitch said:
sometimes yes, sometimes no.

not every fruit is born of a single strain.
strains that have not 'mated' are capable of collaborating to
produce a single fruit or cluster of fruits.

on agar, with clone tissue, often you can see distinct separate
sectors grow and from from the biopsied tissue. 
so as I see it (and who knows if I even have a clue what I'm
talking about...) if you're lucky you can clone an isolate, but most
of the time you'll just be reducing the strains to two of them or so.
fruits found in the wild have a greater chance of being born of
a greater number of strains.  so you would have an even smaller
chance of cloning an isolate from the wild.  home fruits would be
your best bet.

so after cloning the fruit to agar, further isolate from there, and
grow out each sector on it's own and fruit side by side.  keep the
one you like the best and toss the other one.

(if I even know what I'm talking about... :naughty: )

lately I cloned a PE fruit that heavily sporulated...
the cloned culture grows produced about 1 in 200 fruits that
produced spores....
so it's not always as easy as it might sound.
time to go into further isolation....




thank you so much! i have been trying to understand this for the longest time and after reading every tek and archieved info, i've just become more conufsed.


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