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wutang
fungi



Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
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how cloning works
#7544324 - 10/21/07 07:52 PM (16 years, 3 months ago) |
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well i made a few successful agar clones. great. easy. but WHO EXACTLY KNOWS how it works?? i got a monster pin thats going to be a monster shroom, but im so confused!!!!!  does it mean that once you transfer a wedge to spawn and inoculate then fruit. will all the shrooms that pop up be like just like the one i transfered to agar? same size? ect. when i hear cloning thats what i think of.
btw i searched articles on agar but only found how to teks
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syntroniks
Stranger Danger
Registered: 10/18/07
Posts: 76
Loc: Assen, Holland
Last seen: 16 years, 1 month
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Re: how cloning works [Re: wutang]
#7544373 - 10/21/07 08:01 PM (16 years, 3 months ago) |
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I think that is generally how it is, but I wouldn't think they would all be exactly the same. Now if you cloned the shorted shroom then fruited and cloned that one's shortest and so on, you'd get short shrooms I know that
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shroomerite
Apprentice


Registered: 06/09/06
Posts: 513
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Re: how cloning works [Re: syntroniks]
#7544393 - 10/21/07 08:06 PM (16 years, 3 months ago) |
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Yeah, basicly you get a sort of "substrain" isolate the has common fruiting capabilities. The goal is to isolate a good fruitng strain that way you get consistant fruits and potency along with more even pinsets.
-------------------- "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR
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monstermitch
Growing in Bags Doesn't Work



Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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sometimes yes, sometimes no.
not every fruit is born of a single strain. strains that have not 'mated' are capable of collaborating to produce a single fruit or cluster of fruits.
on agar, with clone tissue, often you can see distinct separate sectors grow and from from the biopsied tissue. so as I see it (and who knows if I even have a clue what I'm talking about...) if you're lucky you can clone an isolate, but most of the time you'll just be reducing the strains to two of them or so. fruits found in the wild have a greater chance of being born of a greater number of strains. so you would have an even smaller chance of cloning an isolate from the wild. home fruits would be your best bet.
so after cloning the fruit to agar, further isolate from there, and grow out each sector on it's own and fruit side by side. keep the one you like the best and toss the other one.
(if I even know what I'm talking about... )
lately I cloned a PE fruit that heavily sporulated... the cloned culture grows produced about 1 in 200 fruits that produced spores.... so it's not always as easy as it might sound. time to go into further isolation....
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wutang
fungi



Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
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very helpful advice mitch and very interesting how a cloned PE that sporulated still didnt produce any that did.
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somebody041
bud tester


Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
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Quote:
monstermitch said: sometimes yes, sometimes no.
not every fruit is born of a single strain. strains that have not 'mated' are capable of collaborating to produce a single fruit or cluster of fruits.
on agar, with clone tissue, often you can see distinct separate sectors grow and from from the biopsied tissue. so as I see it (and who knows if I even have a clue what I'm talking about...) if you're lucky you can clone an isolate, but most of the time you'll just be reducing the strains to two of them or so. fruits found in the wild have a greater chance of being born of a greater number of strains. so you would have an even smaller chance of cloning an isolate from the wild. home fruits would be your best bet.
so after cloning the fruit to agar, further isolate from there, and grow out each sector on it's own and fruit side by side. keep the one you like the best and toss the other one.
(if I even know what I'm talking about... )
lately I cloned a PE fruit that heavily sporulated... the cloned culture grows produced about 1 in 200 fruits that produced spores.... so it's not always as easy as it might sound. time to go into further isolation....
thank you so much! i have been trying to understand this for the longest time and after reading every tek and archieved info, i've just become more conufsed.
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