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Re: Morel and morel excited! [Re: strangladesh]
    #6944027 -

The morel jar seems to be getting cob web mold.


The morel mycobag has growth that is off white and becoming orange-ish.


The morel LC liquid became non-clear also indicating bacterial cotam, but then somehow seems to be recovering.


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6944058 -

Here is an updated pic of the morel mycobag:



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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! *DELETED* [Re: The_Jackal]
    #6946771 -

Post deleted by Morelman

Reason for deletion: Never again...



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Re: Morel and morel excited! [Re: Morelman]
    #6947751 -

Morelman said:
Your jar looks fine. The grain always looks "fuzzy" at this stage. The mycelium will start to turn brown soon.


Thanks for your expertise!


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7013116 -

Trying some morel agar work with:

Rose Bengal agar Plates

Description: 1.0% Glucose, 0.5% peptone, 0.1% potassium phosphate (monobasic, anhydrous), 0.05%magneium sulfate, heptahydrate, 0.003% Rose bengal, and 2.0% agar.
Applications: Used for isolating fungi. Designed to suppress bacterial growth.

Not sure if the rose bengal pre-poured agar was a good choice for morel isolations. It seems to inhibit the growth of anything.

It's been two days and nada. How long does it take for the morel cultures to spread on agar from a drop of LC?



Sneezed on a couple of plates for shits and giggles to see if anything will grow on this stuff. The recipe stated "Used for isolating fungi," but it just may be a loose nut behind the wheel (yours truly).

Meanwhile, the mycobag and jar seem to be growing some ball like structures that based on the below pic from http://www.fungaljungal.org/morels/mofire.htm appear to be morel sclerotia.



Will let them go for a couple more weeks to see what happens.

Cheers!

Jackal


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7017923 -

"Prepare[d 10 cups of] the "supersoil" substrate, the standard mixture is as follows:

20 percent sand [2 cups lava sand],
30 percent soil [3 cups top soil],
50 percent organic material [5 cups] composed of [:]

80 percent small wood chips [4 cups apple wood chips],
10 percent rice hulls [1/2 cup],
5 percent soybean meal [1/4 cup],
5 percent sphagnum [1/4 cup].

Correct[ed] the pH with lime to 7.1-7.3 [using Kelway soil tester]. Mix[ed] well [in 1 gallon ziplock baggie]."



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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7018978 -

Your'e doin a great job on this experiment Jackal. I hope to try this out someday aswell if you can perfect the technique for us indoor grower's.


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“I believe that water will one day be employed as fuel, that hydrogen and oxygen which constitute it, used singly or together, will furnish an inexhaustible source of heat and light, of an intensity of which coal is not capable.” – Jules Verne, T

“Ere many generations pass, our machinery will be driven by power obtainable at any point in the universe...it is a mere question of time when men will succeed in attaching their machinery to the very wheel-work of nature."
- Nikola Tesla


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Re: Morel and morel excited! [Re: buddhahoodlum] * 1
    #7019506 -

buddhahoodlum said:
Your'e doin a great job on this experiment Jackal. I hope to try this out someday aswell if you can perfect the technique for us indoor grower's.


Thanks, mang!

If I get just one fruit, I will be happy!


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7025017 -

"Fill[ed] an autoclavable aluminum [20.2 cm dia x 8.0 cm] tray (i.e. c[asserole] pan)
(liberally punched with drainage holes) to a depth of 2 inches with [supersoil] substrate.
Step 17.
Saturate[ed] substrate thoroughly with water [for 24 hrs].
Allow[ing] to drain completely. Fill[ing] a second, identical tray with soaked [for 24 hrs], [and] drained rye grass seed to a depth of 1/2 - 1 inch."

Will be using oven bags with 1/2 plastic tube polyfil filters for forthcoming PCing step.






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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7026641 -

Interesting!!!
Good Luck!!!


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MG

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Re: Morel and morel excited! [Re: The_Jackal]
    #7029536 -

Jackal, you've got to assume that even if you reproduce the conditions exactly it still might not work. You should have at least 10 trays and try different springtime like triggers. Don't forget there are some known super-weeds that
are growing in the same places as the morels, such as garlic mustard, its a foreign invader and it has been spreading like wild-fire. A plant like that would deplete the soil of nutrients quickly. So you should try a tray with a plant like that, seeded along side the mushroom, when its time to "chill", "freeze".


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If you wanna ride, don't ride the white horse, if you wanna ride, ride the white pony

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Re: Morel and morel excited! [Re: AcidHorse]
    #7029688 -

AcidHorse said:
Jackal, you've got to assume that even if you reproduce the conditions exactly it still might not work. You should have at least 10 trays and try different springtime like triggers. Don't forget there are some known super-weeds that
are growing in the same places as the morels, such as garlic mustard, its a foreign invader and it has been spreading like wild-fire. A plant like that would deplete the soil of nutrients quickly. So you should try a tray with a plant like that, seeded along side the mushroom, when its time to "chill", "freeze".


Good points! However, I am all set for defeat with this first shot at it, but it will have been a fun experiment nonetheless!


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7029709 -

"Step 18.
In a clean (use[d] 5% bleach to clean up) draftfree
area [sprayed with Oust, cleaned with alcohol]
open[ed] cooled substrate bag and mix[ed] [38.9 g]
[of sclerotia] into substrate using [alcohol wiped surgical gloves].
Reclose[ed] bag and [being] place[ed] in a cool (65-70°F) dark place for [next] 4-6 weeks.
During this period (the spawn run) the relative humidity [will] be kept at
90-100%, CO2 [uncontrolled] at 6000-9000 ppm, and
no fresh air exchanges [due to being sealed in oven bag]."











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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7088597 -

Update:

substrate tray appears to be colonizing with white fuzz growing from sclerotia innoc points into rest of substrate. Hopefully not contams. Spawn run to last 4-6 weeks ending 7/8-7/22.

Edited by The_Jackal (06/24/07 08:19 PM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #7092110 -

Cool man!


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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Re: Morel and morel excited! [Re: Jeremy_Davis]
    #7114567 -

Jeremy_Davis said:
Cool man!


Thanks bro!

Here are updated pics from 6/26:



and 7/1:



--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7171785 -

Here is updated pic! Looks like winter is right around the corner.



--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7177196 -

Looking good man, I've been following this for ages, and I assume there are many others watching also.

Have you found this that difficult, or do you think that the problem lied in finding out the method, and not with actually carrying out the procedure?

Hope it all goes well! :grin:

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Re: Morel and morel excited! [Re: Feelers]
    #7178721 -

Feelers said:
Looking good man, I've been following this for ages, and I assume there are many others watching also.

Have you found this that difficult, or do you think that the problem lied in finding out the method, and not with actually carrying out the procedure?

Hope it all goes well! :grin:


Thanks buddy!

In answer to your questions, the method seems fairly straight forward to execute so far.  However, the further execution of the method may be difficult, due to the tight control of temp, rh, etc., and the means needed to carry this out.  I suspect having a modified walk in refrigerator or the like with some hard wired temp, rh, etc., control would be the way to go.

As an update, was inspecting the top substrate  tray and it seems that almost the entire top layer is covered with hard, rock-like, rust colored sclerotia  formations!

Will let it go as long as possible to make sure the entire substrate  is colonized and while waiting on a Coleman thermoelectric cooler to arrive this Monday and to serve as the winter home for the sclerotia.

Will take lots of pics next week during the next step of removing the rye  grass tray nutrient source and placing the substrate  tray into its winter home for a couple of months.

Cheers!

Jackal


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #7186129 -

Impatience got the better of me so here goes:

"Step 20.
CHILLING (a necessary step): After spawn run, remove[d] rye seed tray from bag, reclose[d], and place[d] bagged substrate tray into refrigeration (38-40°F) for [next] two weeks."

substrate and nutrient tray ready for extraction


substrate tray close up


nutrient tray close up


substrate tray sealed up and nutrient tray


nutrient tray sealed up for further colonization of spawn if needed


substrate tray in its winter home



Cheers!

Jackal


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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