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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Re: Morel and morel excited! [Re: The_Jackal]
    #6803238 -

Nah man, we're all one family here, working towards the same thing. I'm just the hippie kid that you can't get into the shower I guess:grin:

I'm just way into low-tech. That's my thing for myself, but I love to see how complex it can get. I've done some pretty techy grows too, I guess, now that I think about it...


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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Re: Morel and morel excited! [Re: Jeremy_Davis]
    #6815242 -

The vortex tube worked down to 40F in an insulated cooler, but will only be feasible with a large (80 gallon or so) air storage tank, so ditched that idea. Instead, hooked up an a/c (pic 1) to the greenhouse for cooling (as suggested by RR). Also added a HEPA filtered fan to suck in the cold air from the a/c (pic 2) for HEPA filtered a/c and extreme FAE . Also, added a heat diffuser made from a sheet to the a/c exhaust output (pic 3). Put both HEPA fan and a/c on computer control for morel fruiting cycle (pic 4).





The above setting was for testing the extreme of temps and RH , taken from the article which suggested this simplified pattern:

"[innoc then] 69-71 degrees F.
Four to six weeks later you should observe whitish mycelial.
After five weeks, you should also see white to rust colored masses forming. These are the sclerotia.
inoculate with about 1,500-4,000 cc sclerotium per meter of substrate.
Refrigerate for about one month at temperatures just above freezing (35-40 degrees F).
Allow the substrate to return to 65-70 degrees F.
"Flood" with sterile H2O. Leave for 24 hours.
apply casing to 1/2 inch thick.
Allow 7-10 days @ 65-70F for formation of primordia . Location should be dark with one to two air exchanges per hour.
Adjust your substrate to 60 percent moisture, relative humidity to 85-95 percent, air temperature to 70-73 degrees F., and air exchanges to six to eight per hour. Light should be maintained on an on/off 12 hour cycle.
In three to seven more days, adjust parameters for full fruit-body maturation: substrate moisture 50 percent, relative humidity 85 percent, air temp 10-15 degrees C., and fresh air exchange at 6 to 8 per hour.
Once your mushrooms have reached a length of 126 mm, you may justifiably break out the Dom Perignon, and party on!"



--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (04/25/07 12:16 AM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6830200 -

Cannot locate the supersoil :

"Prepare the "supersoil" substrate , the standard mixture is as follows: 20 percent sand, 30 percent soil, 50 percent organic material composed of 80 percent small wood chips, 10 percent rice hulls, 5 percent soybean meal, 5 percent sphagnum. Correct the pH with lime to 7.1-7.3."

So ended up ordering everything ala carte!


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6844805 -

Got some more computer controlled switches and now heat and ac (and soon simulated heavy rainfall) are on computer control.


--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (04/28/07 10:55 AM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6848900 -

Started morel LC run today.

Procedure was to make LC solution, shut down greenhouse fans, cool mist, ultrasonic , heaters, ac, etc., spray the inside of greenhouse liberly with 70% iso alcohol, wipe everything down, and introduce working equipment, scissors, tape, inoculation loop, LC jars, morel culture syringe, etc.


Then, used greenhouse glove box features to perform still air LC inoculation.


Finally, greenhouse was put in 75F incubation mode.


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6864076 -

All supplies are in and the morel LC is going good.

Did some rainfall experiments by hooking up two 2.5 gallon water containers together, one with a pump for pumping water back into the other container. All that is needed now is to make a large shower head connected to the output hose with the pump hooked up to a cpu controlled switch for programmable rainfall.

Why do I feel like Macgyver?









--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6866465 -

Made shower head out of plastic container and tested on remote control for proof of concept. Let there be rain!







Rainwater system fitted inside of greenhouse. Will make bigger shower head for watering of actual morel substrate container.



--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (05/03/07 09:12 AM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6867454 -

I to will be trying this but i think genetics will be my first step...multiple cultures from the wild find out which one produces the easies...i will see which on produces sclerotium first and primordia...

I really think genetics is the key to these mushrooms...


--------------------
Buy this Car
http://cgi.ebay.com/ebaymotors/ws/eBayISAPI.dll?ViewItem&rd=1&item=330166869870&ssPageName=STRK:MESE:IT&ih=014

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Re: Morel and morel excited! [Re: strangladesh]
    #6870313 -

strangladesh said:
I to will be trying this but i think genetics will be my first step...multiple cultures from the wild find out which one produces the easies...i will see which on produces sclerotium first and primordia...

I really think genetics is the key to these mushrooms...


Sounds good, I wish I had access to wild cultures. So far, only have access to a syringe of Black morel from sporeworks, and possibly will be getting a wild Grey morel culture from a friend.


--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (05/03/07 11:51 PM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6871532 -

I wish I had an agar kit handy, I could clone a bunch that fruited in my outdoor patch. Im going to make sure I have everything I need to clone before next season.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Morel and morel excited! [Re: Psychoslut]
    #6871909 -

Just save some dry specimens in a ziploc to use for cloning. All of the dry Morchella that i've attempted to revive have been a sinch.

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Re: Morel and morel excited! [Re: Hotnuts]
    #6871971 -

thanks ill go dry a few now. How did you do it? Did you soak the dry morel or just put a dry piece on agar?


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Edited by Psychoslut (05/04/07 11:38 AM)

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Re: Morel and morel excited! [Re: Psychoslut]
    #6872037 -

I break a small section of the stem off in the ziploc and then I crumble it up a bit. Then you can take a sterile knife and use that to scoop out the crumbled tissue. Apply them dry across the agar. Usually within 5 days you'll start to see growth from the mushroom's tissue, or before that. Morchella is very fast! You will have mold as well, so you'll have to make a few transfers to new plates to get away from it. But it's easy to get away from because the Morchella is so fast to colonize.

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Re: Morel and morel excited! [Re: Hotnuts]
    #6875447 -

Hotnuts said:
I break a small section of the stem off in the ziploc and then I crumble it up a bit. Then you can take a sterile knife and use that to scoop out the crumbled tissue. Apply them dry across the agar. Usually within 5 days you'll start to see growth from the mushroom's tissue, or before that. Morchella is very fast! You will have mold as well, so you'll have to make a few transfers to new plates to get away from it. But it's easy to get away from because the Morchella is so fast to colonize.


Can you go straight to LC with the dry culture tissue? Maybe after peroxide bath?


--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (05/05/07 09:37 AM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6875467 -

Started morel spawn.

"Making spawn
5 parts annual rye grass seed (or other grain)
1 part potting soil

Cover grain with water. Soak 24 hours. Drain. Mix with potting soil. Place 2cc of this into each 1 qt canning jar with micro-porous filter in the lid. Sterilize 1 hr @ 15 psi in a pressure-cooker.

Once cooled, the usual sterile transfer techniques can be used to insert a few small pieces of Mycelium from your agar culture into the spawn jars. Reseal. Cover with foil. Shake and store in dark, cool (69-71 degrees F.) location. Four to six weeks later you should observe whitish Mycelial Threads throughout the medium. After five weeks, you should also see white to rust colored masses forming. These are the sclerotia."

Procedure was to make (1) custom spawn jar with injection port and whatman filter, and (2) mycobag with injection port and filter (purchased from sporeworks), fill with spawn mixture, and PC 1.5 hrs at 15 PSI.

Then, put greenhouse in glove box mode: shut down greenhouse fans, cool mist, ultrasonic , heaters, ac, etc., spray the inside of greenhouse liberally with 70% iso alcohol, wipe everything down, and introduce working equipment, LC jars, syringes, alcohol wipes, etc.

Then, used greenhouse glove box features to prepare four 10 CC culture syringes from LC, and perform still air LC inoculation of spawn jar and mycobag (20 CCs each). Finally, greenhouse was put into incubation mode, at 70F, 80% RH, and no light.

Stay, tuned, morel to come!







--------------------
"The only easy day was yesterday!"--Navy SEALs

Edited by The_Jackal (05/05/07 09:46 AM)

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Re: Morel and morel excited! [Re: The_Jackal]
    #6875787 -




Can you go straight to LC with the dry culture tissue? Maybe after peroxide bath?


No. You'll need to use agar.

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Re: Morel and morel excited! [Re: Hotnuts]
    #6875870 -

Hotnuts said:

Can you go straight to LC with the dry culture tissue? Maybe after peroxide bath?


No. You'll need to use agar.


Do you know why that is?


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6877383 -

i have 10 different strains of morel going now....thanks to my last hunt hehe hopefully i stumble upon that easy to fruit strain no one has found yet and become a millionaire....


--------------------
Buy this Car
http://cgi.ebay.com/ebaymotors/ws/eBayISAPI.dll?ViewItem&rd=1&item=330166869870&ssPageName=STRK:MESE:IT&ih=014

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Re: Morel and morel excited! [Re: strangladesh]
    #6886774 -

strangladesh said:
i have 10 different strains of morel going now....thanks to my last hunt hehe hopefully i stumble upon that easy to fruit strain no one has found yet and become a millionaire....


Gluck! I have about 5 or so strains to work with now.


--------------------
"The only easy day was yesterday!"--Navy SEALs

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Re: Morel and morel excited! [Re: The_Jackal]
    #6886981 -

We should exchange a few...i just got some new ones from about 1hr from my house we picked about 4 differnt kinds out there...some really cool tiny ones..and some very big blacks...


--------------------
Buy this Car
http://cgi.ebay.com/ebaymotors/ws/eBayISAPI.dll?ViewItem&rd=1&item=330166869870&ssPageName=STRK:MESE:IT&ih=014

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Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures   MagicBag.co Certified Organic All-In-One Grow Bags

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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