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OfflineMurit
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Easy Needle Sterilization method?
    #5836746 - 07/08/06 08:15 PM (17 years, 6 months ago)

okay...from what I've been reading here on shroomery, a rather large percentage of contaminations happen simply because someone gets careless with their needle, and it becomes unsterile after they've flame sterilized it...well...here's my idea to safe-guard against this...let me know what you guys think.

After flame sterilizing the needle, simply take an alcohol swab and lay it over your pin hole on your cake...then simply stick the needle straight through the swab and into your cake.
Think this would work?


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and violent reactions..
..in those who have never tried them."
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Offlinejcee
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Re: Easy Needle Sterilization method? [Re: Murit]
    #5836835 - 07/08/06 08:42 PM (17 years, 6 months ago)

yes


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OfflineHippieChick
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Re: Easy Needle Sterilization method? [Re: Murit]
    #5836882 - 07/08/06 09:02 PM (17 years, 6 months ago)

My quart lids for my WBS are actually made with a poly fil plug that I soak with alcohol and then inoculate through it . I never flame my needle . Just wrap it in an alcohol swab and go to town . Open air inoculation , no flame , no problems :thumbup:

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


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Freedoms just another word for nothing left to lose..............

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OfflineMurit
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Re: Easy Needle Sterilization method? [Re: HippieChick]
    #5836897 - 07/08/06 09:08 PM (17 years, 6 months ago)

awesome. thanks.

oh, and HC, I've been wantin to ask...what did you use to grow that monster of a shroom in your avatar?


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"Halucinogens produce severe
and violent reactions..
..in those who have never tried them."
- Terence McKenna


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OfflineHippieChick
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Re: Easy Needle Sterilization method? [Re: Murit]
    #5836907 - 07/08/06 09:12 PM (17 years, 6 months ago)

That is SA grown on Tenn. Stud Pre-Pasteurized H/Poo. :thumbup: Started from a LC .

Good Luck

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisiblefastfred
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Re: Easy Needle Sterilization method? [Re: HippieChick]
    #5837066 - 07/08/06 10:06 PM (17 years, 6 months ago)

A good tip I heard was to use a piece of sponge soaked in alcohol and put your needle through it. Then when you inject the hole the sponge gets pushed back, and when you remove the needle the sponge again covers it.

Pretty slick huh?


-FF


Edited by fastfred (07/08/06 10:32 PM)


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OfflineRogerRabbitM
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Re: Easy Needle Sterilization method? [Re: fastfred]
    #5837148 - 07/08/06 10:30 PM (17 years, 6 months ago)

The problem is the alcohol doesn't penetrate to the inside of the needle. I suggest flaming the needle red hot, then using the first couple of drops from the syringe to cool it down. That way, you know the inside and outside of the needle are sterile. In other words, heat it red hot, then allow to cool for only a few seconds and use.
RR


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Invisibleliamtheloser
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Re: Easy Needle Sterilization method? [Re: RogerRabbit]
    #5837171 - 07/08/06 10:34 PM (17 years, 6 months ago)

amen, rr. that's always been my concern with straight alcohol sterlization without flaming... is that there's about an inch and a half of potentially exposed liquid sitting in that needle... i flame then wipe with alcohol just so the carbon doesnt get all over the place.


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OfflineRogerRabbitM
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Re: Easy Needle Sterilization method? [Re: liamtheloser]
    #5837401 - 07/08/06 11:29 PM (17 years, 6 months ago)

Use denatured alcohol from the paint department and there won't be any carbon. You get the soot from using rubbing alcohol.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineOatman2000
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Re: Easy Needle Sterilization method? [Re: RogerRabbit]
    #5839104 - 07/09/06 03:00 PM (17 years, 6 months ago)

my needle tips start to rust once i am done flame sterelizing...

i don't flame them anymore.


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Offlinetonyperez420
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Re: Easy Needle Sterilization method? [Re: RogerRabbit]
    #5839566 - 07/09/06 05:12 PM (17 years, 6 months ago)

Quote:

RogerRabbit said:
The problem is the alcohol doesn't penetrate to the inside of the needle. I suggest flaming the needle red hot, then using the first couple of drops from the syringe to cool it down. That way, you know the inside and outside of the needle are sterile. In other words, heat it red hot, then allow to cool for only a few seconds and use.
RR




Listen to RR

he knows his shit


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