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OfflineKilljoy
TheHyperdimensionalSlug

Registered: 01/28/03
Posts: 865
Last seen: 2 years, 3 months
Sterilizing syringes??? QUESTION:
    #1405844 - 03/24/03 12:20 PM (13 years, 8 months ago)

I've got some 12cc syringes, I used one to innoculate 8 jars today (set the cap on a sterilized shotglass overnight, sucked up a syringe full of distilled water, shot it in there, mixed it all up, sucked up the spore water, then innoculated)...

I'm wondering what the easiest least fuck-around method is for sterilizing syringes.  I know about boiling water and sucking it up multiple times, and letting it sit and cool and all, but there has got to be a less labor intensive way to do this.......

Would it work well if I just rinsed them with distilled water, filled them with distilled water, then wrapped them in tin-foil and PC'd them for an hour?  Has anyone done this, and would it mess up the syringes at all?  And would it just be easier to fill them with water and microwave them for a bit?

Thanx shroomerites! :smile:


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Anonymous

Re: Sterilizing syringes??? QUESTION: [Re: Killjoy]
    #1405852 - 03/24/03 12:23 PM (13 years, 8 months ago)

that syringe making tek sounds pretty dirty.

you can PC syringes to sterilize them. i think steaming for an hour would also be sufficient. don't microwave them. they have metal on them !


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OfflineCosmicTourist
Stranger
Registered: 02/25/03
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Last seen: 11 years, 9 months
Re: Sterilizing syringes??? QUESTION: [Re: Killjoy]
    #1405886 - 03/24/03 12:35 PM (13 years, 8 months ago)

just boil some water in the microwave, and purge the syringe with that. You could take the syringe apart and throw the whole thing in a bowl filled with water and boil it too.


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Offlinedelsloow
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Registered: 01/29/03
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Re: Sterilizing syringes??? QUESTION: [Re: ]
    #1405890 - 03/24/03 12:37 PM (13 years, 8 months ago)

Quote:

don't microwave them. they have metal on them !




i found this out the hard way today. They made some interesting flashes in the microwave though. Then i re-read over the tech and noticed that you should suck up 1cc of water, then suck up the remaining 9 cc or 11 cc full of air. This stopped the metal from melting. This seemed to steralize it pretty well, i put them in there for 5 minutes and it was all steamy inside of there


Edited by delsloow (03/24/03 12:38 PM)


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Offlinesi1988crx
Beep beep byebye

Registered: 03/08/03
Posts: 267
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Re: Sterilizing syringes??? QUESTION: [Re: delsloow]
    #1405961 - 03/24/03 01:06 PM (13 years, 8 months ago)

I have never had a problem with the metal melting. I wonder how hot your water is? I bring a pot of water to a boil, seperate the syringe and needle and drop both in. I let it boil for 5-10 minutes then take out the syringe and needle, put them back together, and then suck up 12cc of the boiling water twice. After that, I let them cool and before I fill it with spores i wipe the needle down with alcohol and and the same before I innoculate. Takes a little longer, but why risk it when you can spend an extra 5 minutes and be amost absolutely sure that they are sterile.

Just make sure that the plastic will not melt, most syringes will take the heat, but some cheap ones might not.


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OfflineKilljoy
TheHyperdimensionalSlug

Registered: 01/28/03
Posts: 865
Last seen: 2 years, 3 months
Re: Sterilizing syringes??? QUESTION: [Re: si1988crx]
    #1406593 - 03/24/03 05:24 PM (13 years, 8 months ago)

I know it sounds dirty, and I was a bit lazy, but we'll see how it turns out, and I'll let you know.....

If my assumption that distilled water from the store is sterile, then I should be fine.


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Invisiblepoke smot!
floccinocci floofinator
Male

Registered: 01/08/03
Posts: 5,231
Re: Sterilizing syringes??? QUESTION: *DELETED* [Re: Killjoy]
    #1406727 - 03/24/03 06:32 PM (13 years, 8 months ago)

Post deleted by poke smot!

Reason for deletion: x



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Offlineshoe
In Death'sEmbrace
Registered: 03/20/03
Posts: 26
Loc: Australia
Last seen: 13 years, 7 months
Re: Sterilizing syringes??? QUESTION: [Re: poke smot!]
    #1406756 - 03/24/03 06:50 PM (13 years, 8 months ago)

I've been wondering about this... woudln't the syringes melt in the boiling water...? They seem to be made of plastic, is it high temperature resistant?


--------------------
The above writings are fictional. It is being used to collect information to write a book about someone growing mushrooms.

And remember I will always love you,
As I claw your fucking throat away.
It can end no other way.


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Invisiblegeorge castanzaM
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Trusted Cultivator
Re: Sterilizing syringes??? QUESTION: [Re: shoe]
    #1407167 - 03/24/03 11:46 PM (13 years, 8 months ago)

Would it work well if I just rinsed them with distilled water, filled them with distilled water, then wrapped them in tin-foil and PC'd them for an hour? Has anyone done this


this is the only way kramer sterilizes his syringes and he says it works just fine


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

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Offlinejimsuzo
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Registered: 08/14/02
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Re: Sterilizing syringes??? QUESTION: [Re: Killjoy]
    #1407220 - 03/25/03 12:48 AM (13 years, 8 months ago)

Pressure cooking as you describe works fine for the relatively 'soft' plastic syringes, but it will melt the harder, more brittle type.
The rubber and metal bits have never caused a problem for me.


--------------------
There are roughly nine galaxies for every person alive on the planet today. Each of these galaxies has a billion suns, give or take the odd hundred million


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
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Re: Sterilizing syringes??? QUESTION: [Re: jimsuzo]
    #1407393 - 03/25/03 03:28 AM (13 years, 8 months ago)

BD syringes will hold up fine to PC'ing.

I fill mine with javex, let them sit for a bit. purge them out, refill with clean water a few times to rinse bleach out of them.. then fill with bottle water and wrap in foil, pc them along with a batch of jars, and bingo,

Done.


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Offlinemikejwill
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Registered: 01/22/03
Posts: 167
Loc: Midwest,usa
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Re: Sterilizing syringes??? QUESTION: [Re: Killjoy]
    #1407418 - 03/25/03 03:48 AM (13 years, 8 months ago)

Quote:

but there has got to be a less labor intensive way to do this.......

Would it work well if I just rinsed them with distilled water, filled them with distilled water, then wrapped them in tin-foil and PC'd them for an hour?




lol dude i have no idea how you think that it's easier to pc them....sucking up boiling water IS easy. i wouldn't microwave the syringes, it might fuck them up.


my question is though....if the syringe is sterile to begin with, then why not just buy a new bottle of distilled water and suck it up quickly? that would keep it all sterile wouldn't it?

peace and pot,
mike's dog


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Sterilizing syringes??? QUESTION: [Re: mikejwill]
    #1407607 - 03/25/03 05:58 AM (13 years, 8 months ago)

Quote:

why not just buy a new bottle of distilled water and suck it up quickly? that would keep it all sterile wouldn't it?



Distilled does not = sterile.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Amazon Shop for: ½ Pint Jars

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