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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Rye Grain, Vermiculite

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Offlinesillvyr
member

Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 8 years, 5 months
Problems Encountered During Inoculation
    #528230 - 01/22/02 02:37 PM (14 years, 10 months ago)

Hello Again!
Well, yesterday was the big day, the jars were inoculated. I have been second-guessing myself since I finished, though. I think I may have made some big mistakes, though I hope it'll be OK. This is what happened. I warmed the oven up to between 150 and 200 degrees. The kitchen was cleaned beforehand, and I doused the entire room in Lysol spray. I took a shower, put on clean clothes, put on mask and covered my hair with a clean rag tied on my head. I prepared a syringe from a print, but I couldn't get the spores to come off the print. I panicked and soaked the print IN my jar I had the water in. This seemed to get the spores off the paper, but I hope I didn't make a bad choice in doing this. The second problem I encountered was that the needle of the syringe I bought at the feed store maybe wasn't long enough. I inoculated all the jars, but then made this realization. I made a second syringe, opened the jars and pushed the syringe through the Ramsey Seal (vermiculite in foil "pond" in jar top) and injected the spores, however I'm now concerned that the spores were introduced below the surface of the substrate. Is this bad news? I sterilized needle before every injection with flame.
I am really anxious about messing this up. I now have my jars in the box the jars came in (to block light) incubating in my terrarium. The terrarium is under a tent of a clean sheet and I'm heating the room with a space heater, and in front of the heater I have set up a clothes drying rack that I hang wet clean towels on to help create ambient humidity so that the room stays contant and whatever small air exchange goes on doesn't dry the jars out.
Is this going to work, or did I already mess up?


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The "Little Mushrooms" are but emisaries to the Overmind.....



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Anonymous

Re: Problems Encountered During Inoculation [Re: sillvyr]
    #528249 - 01/22/02 03:04 PM (14 years, 10 months ago)

A lot of overkill!

Your problem may be in that you dipped the syringe paper in the water to get the spores off. That paper was likely contamined.


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Offlinesillvyr
member

Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 8 years, 5 months
Re: Problems Encountered During Inoculation [Re: ]
    #528256 - 01/22/02 03:14 PM (14 years, 10 months ago)

I guess now I must wait and see iI messed up. I sure hope not. I may have just wasted my time & effort.


--------------------
The "Little Mushrooms" are but emisaries to the Overmind.....



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Offlinesillvyr
member

Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 8 years, 5 months
Re: Problems Encountered During Inoculation [Re: ]
    #528260 - 01/22/02 03:15 PM (14 years, 10 months ago)

I guess now I must wait and see if i messed up. I sure hope not. I may have just wasted my time & effort. Better prepare for the worst--disappointment!


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The "Little Mushrooms" are but emisaries to the Overmind.....



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Invisiblemrdasani
enthusiast
Registered: 10/19/01
Posts: 224
Re: Problems Encountered During Inoculation [Re: sillvyr]
    #528405 - 01/22/02 06:17 PM (14 years, 10 months ago)

You shouldn't have to take a shower and even washing your hands isn't completely necessary. Just sterilize the jars really good, and make sure your syringe is wiped clean with alcohol or burnt with flame. If you spawn jars (like putting a little piece of mycelium in a new jar), becareful this part is pretty risky. Also make sure the room has no wind.


--------------------
"Come on you raver, you seer of visions,
come on you painter, you piper, you prisoner, and shine!" - Pink Floyd (Wish You Were Here 1975)

"Never underestimate the power of denial." -from American Beauty.


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Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 14 years, 7 months
Re: Problems Encountered During Inoculation [Re: mrdasani]
    #528436 - 01/22/02 07:13 PM (14 years, 10 months ago)

all i can say about innoculation problems is the oven tek...simple, easy and highly effective.


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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 14 years, 7 months
Re: Problems Encountered During Inoculation [Re: mrdasani]
    #528943 - 01/23/02 10:19 AM (14 years, 10 months ago)

Why is putting a piece of mycilium into a new spawn jar risky?
If you have it in your laminar flow hood, and dump some of the colonized rye into another try/jar, whats the problem?
I cannot see how contamination could happen.
I do all my inoculation in the flowhood too, flame tools after dipped in alcohol..


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Anonymous

Re: Problems Encountered During Inoculation [Re: cookiewhore]
    #528997 - 01/23/02 12:11 PM (14 years, 10 months ago)

He's not puttng mycellium in, he's putting spores in. Along with the likely contaminated spore paper.


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Offlinesillvyr
member

Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 8 years, 5 months
Re: Problems Encountered During Inoculation [Re: ]
    #529040 - 01/23/02 01:29 PM (14 years, 10 months ago)

CaptainMaxMushroom,
in 2 days, might I see any new growth? if the jars don't grow this time, I guess I'm gonna need new spores, and i guess I'd better get syringes, how much is B+ per syringe at the Sporelab?


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The "Little Mushrooms" are but emisaries to the Overmind.....



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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Rye Grain, Vermiculite

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