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Anonymous

Re: contamination before inoculation?
    #302929 - 04/27/01 06:21 PM (21 years, 5 months ago)

The jars could be perfectly fine but bad syringes could change that.


shroomsi, they are not automatically going to become contaminated just because you opened it for a second unless you live in a morgue full of rotting corpses or a bakery full of mouldy bread.

Or maybe Mexico City.

Take a trip to the Spore Lab @:
http://www.SporeLab.com


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OfflineTaz
veteran
Registered: 03/19/01
Posts: 1,090
Last seen: 21 years, 20 days
Re: contamination before inoculation? [Re: ]
    #303341 - 04/27/01 06:31 PM (21 years, 5 months ago)

I agree with max on this one, I always open mine up and smell, if it smells sour it is contams and must be thrown, contams will show up in 2 to 3 days if it is not serilized right......



--------------------
"Most of the world's problems are caused by people taking things that do not belong to them..."


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Offlineshroomsi
newbie
Registered: 04/07/01
Posts: 11
Loc: UK (the best country)
Last seen: 20 years, 3 months
Re: contamination before inoculation? [Re: ]
    #304949 - 04/29/01 08:44 PM (21 years, 5 months ago)

CaptainMax Mushroom, i know yr always right so i aint gonna argue. But i left my jars standing for 2 weeks = fine. When I opened them for a split second to have a sniff then closed them 2 days later - green mold. This was after cleaning my room, spraying the air, cleaning evrything, cleaning the desk, my hands and fressh clothes.

Oh yes, if you wondering y i opened them, it was purely a test to c if my demo substarte was alrite. Any help on sterilization would help.

Would lighting a blow torch in my room help (burning contanms, i dunno?)



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Anonymous

Re: contamination before inoculation? [Re: shroomsi]
    #305008 - 04/29/01 10:21 PM (21 years, 5 months ago)

Innoculate your jars as soon as possible after sterilizing. Opening them for even a few seconds could allow contaminants to enter. Were the lids on super tight, when you open them mold spores could have entered. You should build yourself a small clean box. But try and use the jars quickly, don't let them sit around so long. I have seen contamination occur in clean rooms, with filtered air, Hepa flow hoods, and everything sterilized. It is impossible to get rid of all the contaminants. Your job as a cultivator is to give the spores the best chance possible to succeed. Letting Jars of uninnoculated substrate, sterilized, sit around for two weeks, is not giving it the best chance to succeed. Build yourself a clean box, Small scale rooms are easier to keep clean!!!!!!!! Innoculate faster.



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