Home | Community | Message Board

Kratom Eye
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com Olympus Myco Bulk Substrate   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinepodman19
newbie
Registered: 03/18/02
Posts: 47
Last seen: 22 years, 2 months
inoculation question
    #614803 - 04/21/02 02:37 PM (22 years, 3 months ago)

i have reason to believe that my 1/2 pint jars could be contaminated because i wasnt able to boil them in water for a full hour. is it possible that i could wait a few days (say, a week) to inoculate them to see if any contams appear? or do you have to inoculate right after the jars cool? the jars have not been opened since i boiled them. the lids are sealed tight with a piece of tin foil over the sealing disc and under the threaded ring. i just dont want to waste my syringe and inject it into a bunch of contaminated jars. can someone tell me if this will be alright?

also i'd like to know if i need to seal the holes in the lids back up after i inoculate. is it better to leave the holes uncovered or tape them up? does the mycelium need air to colonize? but will contamination get in if i let them uncovered?

Extras: Filter Print Post Top
InvisibleMushroom_Madness
enthusiast
Registered: 01/10/02
Posts: 261
Loc: Quaint New England Villag...
Re: inoculation question [Re: podman19]
    #614981 - 04/21/02 07:25 PM (22 years, 3 months ago)

podman19 Said: "i have reason to believe that my 1/2 pint jars could be contaminated because i wasnt able to boil them in water for a full hour. is it possible that i could wait a few days (say, a week) to inoculate them to see if any contams appear? or do you have to inoculate right after the jars cool?"

Yes, you can waite a week before innoculation. Just keep them in a dark, cool place.

podman19 Said: also I'd like to know if i need to seal the holes in the lids back up after i inoculate. is it better to leave the holes uncovered or tape them up? does the mycelium need air to colonize?

I presume you have a vermiculite layer covering your substrate, or a Mushroom-Madness Contamination Guard. (Which can be made from tyvek if you wish to make your own). If you have a vermiculite layer, then taping the holes will add an added sense of security. But if you are using contamination guards, you do not have to tape the holes.

The mycelium does need air to colonize, and will receive air whether the hole is taped or not.

I hope this information will help you in your research.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com Olympus Myco Bulk Substrate   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Inoculation question montana 1,354 4 12/16/01 04:19 PM
by Karen
* inoculation question Snoop 1,279 4 07/26/02 05:18 AM
by FanaTEK
* Steaming and Inoculation questions podman19 1,455 7 03/29/02 12:09 AM
by WakingUpLate
* Inoculation Question dentedshrooms 643 7 12/29/02 03:47 PM
by SomeMycologist
* mycelium inoculation question HongKongChewy 1,093 3 08/09/01 02:33 PM
by thegoldenteacher
* Re: contamination sucks a big one funkyballoon 767 2 08/03/00 01:24 AM
by Anonymous
* First timer with Invitro/Neglect Tek Questions mtglu 3,452 7 09/12/23 09:52 AM
by DERRAYLD
* Is it contaminated ? Sacred 1,257 8 04/19/02 12:32 PM
by Sacred

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
947 topic views. 13 members, 49 guests and 102 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.