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OfflineSacred
Stranger
Registered: 04/01/02
Posts: 16
Last seen: 14 years, 6 months
Is it contaminated ?
    #612504 - 04/19/02 01:52 AM (14 years, 7 months ago)

Okay tonight I did one of the dumbest things in my life. I got my spore syringe out ready to innoculate. I pulled the tape off the cap and instead of just pulling it off, I unscrewed it !!!!!! I just wasn't thinking. The cap and hypodermic needle were seperated from the syringe. I realized immediately how stupid I was and screwed it back on. Did I for certain contaminate the liquid inside ? It was exposed to the air for maybe 3 seconds. Although my A/C and all the fans were off. I'm so bummed. I dipped the whole end into a glass of isopropyl alcohol and let it dry before injecting the jars. I have such a bad feeling that every single jar will be contaminated. What do you think ?


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InvisibleTitaniumFox
Stranger
Registered: 04/07/02
Posts: 3
Re: Is it contaminated ? [Re: Sacred]
    #612536 - 04/19/02 02:23 AM (14 years, 7 months ago)

It's all academic at this point, because it either was or wasn't, but here are some things to consider:

To put the needle on the syringe, initially, one must uncover both the end of the needle and the end of the syringe. (Unless, you happen to buy syringes that come with the needle already attached. I've seen those.)

So long as you didn't touch the ends of the needle and syringe with anything, you maintained sterile technique outside of the fact that you opened the end. Exposing an area as small as that to the air for 3 seconds is giving bacteria very little chance to infect anything. Realizing that bacterial contamination rates will vary from building to building, and with different times of year, here's a test that we ran at our molecular biology lab: (10) 120mm agar plates were laid out without their tops for various times 15 seconds, 30s, 1m, 2, 4, 6, 8,10, 12, 15m. After looking at the colonies that grew on the plates, the average inoculation time for the lab was about 1 colony per 115cm^2 per 35 seconds.

Quarterly, this average varies about +/- 5 seconds, but it's still a good estimate for where I work. Your miliage will vary, but I wouldn't be worried about contamination in a span of 3s/1cm^2 as compared to 30s/115cm^2, so long as you maintained an aseptic technique otherwise.

TiFox


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InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Is it contaminated ? [Re: Sacred]
    #612541 - 04/19/02 02:28 AM (14 years, 7 months ago)

Who is gonna win the Super Bowl ?

Guess you will find out......in a week or so.

Sorry to hear that you messed up.... we all do sometime.;)

Hope you didn't get any contams....let us know how things turn out.

Good luck! 


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineParticleMan
enthusiast
Registered: 02/05/02
Posts: 240
Last seen: 14 years, 7 months
Re: Is it contaminated ? [Re: Sacred]
    #612598 - 04/19/02 04:11 AM (14 years, 7 months ago)

where's luvdemshrooms, he should be all over this already........

anyway...... my vote is that you will be ok, if nothing else its some positive thinking for you.


--------------------
___________________________________________________________
"the weekend has landed all that exists now is clubs,drugs, pubs, and parties" - Human Traffic


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Invisibleluvdemshrooms
Two inch dick..but it spins!?


Registered: 11/29/01
Posts: 33,806
Loc: Lost In Space
Re: Is it contaminated ? [Re: ParticleMan]
    #612625 - 04/19/02 05:50 AM (14 years, 7 months ago)

Why is that? It's a valid question, it's in the proper forum (although it could have gone either here or in contams as he was in the process of growing), and we have all done things without really thinking while we were doing them.


--------------------
You cannot legislate the poor into prosperity by legislating the wealthy out of prosperity. What one person receives without working for another person must work for without receiving. The government cannot give to anybody anything that the government does not first take from somebody else. When half of the people get the idea that they do not have to work because the other half is going to take care of them and when the other half gets the idea that it does no good to work because somebody else is going to get what they work for that my dear friend is the beginning of the end of any nation. You cannot multiply wealth by dividing it. ~ Adrian Rogers


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Offline2shrewmen
deep seadwellers

Registered: 03/03/02
Posts: 77
Loc: Bikini Bottom
Last seen: 8 years, 10 months
Re: Is it contaminated ? [Re: Sacred]
    #612632 - 04/19/02 05:58 AM (14 years, 7 months ago)

The shrewmen did the exact same thing, and everything was fine.


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InvisibleTitaniumFox
Stranger
Registered: 04/07/02
Posts: 3
Re: Is it contaminated ? [Re: Sacred]
    #612701 - 04/19/02 09:13 AM (14 years, 7 months ago)

One last thing to note is this: If the luer lock end of your needle or the end of your syringe *did* happen to get contaminated with an airborne particle, it had only one place to go: out, most likely in your first bag.

You will find out in about a week, though. I'd have smiles instead of worry.

TiFox


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OfflineUnder_net
enthusiast
Registered: 12/09/01
Posts: 228
Loc: kentucky
Last seen: 13 years, 10 months
Re: Is it contaminated ? [Re: TitaniumFox]
    #612878 - 04/19/02 01:51 PM (14 years, 7 months ago)

Its probably O.K.


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OfflineSacred
Stranger
Registered: 04/01/02
Posts: 16
Last seen: 14 years, 6 months
Re: Is it contaminated ? [Re: Under_net]
    #612896 - 04/19/02 02:32 PM (14 years, 7 months ago)

Thanks for the words of encouragement everyone. I guess there is nothing to do but wait. I should see mycelium growth within a week ? If there is no funky stuff I should be out of the woods ?


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