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Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars

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Offlinesplasher78
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Registered: 07/31/02
Posts: 1
Last seen: 16 years, 3 months
Help! Not sure if contaminated...
    #812076 - 08/12/02 06:12 AM (16 years, 3 months ago)

Help! I am a first time cultivator on my first batch of substrate jars. I am using 1/2 pint jars, 12 of them. Some of the jars are colonizing, and some aren't. I don't know if the ones that aren't colonizing are contaminated. I've read up on alot of contamination issues but can't distinguish if they are contaminated because I don't see any discoloration whatsoever. I inoculated them using sterile procedures. Could it be that maybe I should give the other jars a little more time to start colonizing? It has only been the 4th day since I have inoculated. I am using the puerto rican spores. When they sent the syringes to me, One was dated 8/1/02 and the other was 7/11/02 I used them both but not sure if the older syringe still had fertile spores. I'd appreciate any and all feedback. Thanks
Splasher78


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InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Re: Help! Not sure if contaminated... [Re: splasher78]
    #812105 - 08/12/02 06:47 AM (16 years, 3 months ago)

yes it can take up to a week for the spres to germinate. also if the jars are showing NO signs of ANY kind of growth it could be a lack of water, maybe over sterilaztion? but i would say since it has only been 4 days, definatly give it more time, this is a hobby that requires a lot of patiance! Id recomend not checking on then for a few days at all and incubating them if you can, you will prolly be very suprised what you will see when you take a look!!

also just for future reference, there is a contamination forum, so just make sure you post in the right place, where you can get the most help! Good Luck!!!



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Offlinequietnick
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Registered: 07/11/02
Posts: 11
Last seen: 15 years, 5 months
Re: Help! Not sure if contaminated... [Re: splasher78]
    #812550 - 08/12/02 10:50 AM (16 years, 3 months ago)

its hard to tell anything about contams with out pics however... this early in the game ts impossible to tell.

give your jars a little bit of neglect

nick


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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 12 years, 1 month
Re: Help! Not sure if contaminated... [Re: splasher78]
    #812575 - 08/12/02 11:02 AM (16 years, 3 months ago)

have you ever thought why the tek says 12 jars? one of the reasons is because it is quite logical for a new-cultivator to mess up and have some contaminated jars, so this way gives a possibility you will also have some healthy ones.

but most of all i would say, if you dont see any bacteria [white liquids or oily liquids inside] you HAVE TO wait for the mycelium to show. dont forget that you are working with REAL RESPECTABLE LIFE, and as such, you have to expect different reactions from different organisms. Not all humans grow the same way, not even brothers!

Have some patience

Kykeon
athens, greece


--------------------
The living ghost of Kykeon


Edited by kykeon (08/12/02 11:04 AM)


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