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Registered: 05/06/01
Posts: 121
Loc: London, UK, Basel, CH or ...
Last seen: 15 years, 23 days
    #355115 - 07/15/01 11:59 AM (22 years, 4 months ago)

I have beene using syringes for a while, all good, no problems. But I just bought a pressure cooker and want to move onto using Rye in quart jars.

Recently I've had some trouble using light prints to make syringes, and seeing as I just bought a PC, why not try agar.

I want to have a go, but dont know where to start, and is it really THAT much better than syringes? I have the MMGG original book and that has some good advice for agar.

So, could all you Agar stars give me some advice on whether its really that much better than syringes? What style to use (Potato water? Malt extract, Yeast??????). Where to start and WHAT is that point of isolating a specific rhizmorph of mycelium on a second plate?


"Your Gimmiky Restraunt - By J.R. Bennigans"

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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 21 years, 8 months
Re: Agar? [Re: Maddoc]
    #355317 - 07/15/01 04:43 PM (22 years, 4 months ago)

Agar is just another tool for the mycologist. It's useful if you want to clone a culture, isolate a strain, prepare a liquid innoculant, store/ship a culture, etc, etc.. just use your imagination.

There are also many ways to prepare agar. Low nutrient recipies (cornmeal) are used for storing cultures, while high energy mixtures (malt, honey, sugar) will accomodate quick colonization. You may want to add some of whatever your end substrate will be if you're planning on innoculating w/ the agar you're preparing. For instance, you might add some hardwood sawdust if you're going to be using the agar to innoculate a woodchip sub.

There could be any number of reasons for isolating a strain. Perhaps you're looking for a fungal strain that produces higher than normal ammounts of anti-biotic enzymes. In this case, you'd do a lot of isolating and measurement taking. What you'd probably want to isolate, though, is a strain that produces nice fruitbodies and/or sclerotia. In this case, it's not a bad idea to work backwards.. Meaning, go w/ a multi-spore innoculation, then clone the nicest fruit(s) on agar. As opposed to isolating individual strains and seeing how they grow out.

Hope this helps.

Talents not shared are not a talents.

Where there's skill, there's a better way..

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Registered: 05/18/01
Posts: 695
Loc: california, orange
Last seen: 21 years, 4 months
Re: Agar? [Re: Maddoc]
    #355426 - 07/15/01 08:47 PM (22 years, 4 months ago)

agar seems like just another step that will get you a contam. learn how to colonize rye with just a print/syringe first and you should also learn how to make a really sterile work area or use a glovebox or something. i dont think using agar will make youre stuff grow too much faster or give better yields. if i wanted to have a huge laboratory and just have closets of colonizing rye then i would use agar so i wouldnt have to keep on making syringes, then id use agar. i think it would help if you knew what the fungus wants like egghead said also because agar isnt one of those things that you can just find a recipe for and it will work good.

..( . y . )
......Y /

the little kridders of nature; they dont know that thyre ugly!

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