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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
this is how we made our azure spawn (pictorial)
    #328552 - 05/29/01 06:29 AM (22 years, 3 months ago)
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The spawn is based on small woodchips that can be bought in a pet-store. It is used as animal bedding but can also be used to smoke fish and meat. These chips are made from beech.



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:29 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328554 - 05/29/01 06:30 AM (22 years, 3 months ago)

these chips are boiled in water...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.


--------------------
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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328555 - 05/29/01 06:31 AM (22 years, 3 months ago)
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until they sink...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:31 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328556 - 05/29/01 06:32 AM (22 years, 3 months ago)
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they are drained in a collander...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:32 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328558 - 05/29/01 06:34 AM (22 years, 3 months ago)
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some oatmeal is added to boost mycelial growth..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:35 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328559 - 05/29/01 06:35 AM (22 years, 3 months ago)
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and mixed in....



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:36 AM.



--------------------
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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328560 - 05/29/01 06:37 AM (22 years, 3 months ago)
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this mixture is filled into autoclavable spawnbags..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:39 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328563 - 05/29/01 06:39 AM (22 years, 3 months ago)
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these are put in the pressue cooker..
(we put a metal lid on top to prevent the bags from blocking the steam valve)



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:40 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328565 - 05/29/01 06:41 AM (22 years, 3 months ago)
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these bags are sterilised for two hours.



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:41 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328570 - 05/29/01 06:47 AM (22 years, 3 months ago)
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after sterilisation the bags cool down in the flow cabinet..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:48 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328572 - 05/29/01 06:49 AM (22 years, 3 months ago)
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azurescens spawn on rye grain..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:50 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328573 - 05/29/01 06:50 AM (22 years, 3 months ago)
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half of one jar is used to inoculate these bags...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:51 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328574 - 05/29/01 06:52 AM (22 years, 3 months ago)
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After spawning the bags are sealed with an impulse sealer...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:52 AM.



--------------------
www.911blogger.com


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328575 - 05/29/01 06:53 AM (22 years, 3 months ago)
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and checked for punctures....after this they are shaken and inubated..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:53 AM.



--------------------
www.911blogger.com


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: this is how we made our azure spawn (pictorial [Re: Una]
    #329020 - 05/29/01 05:54 PM (22 years, 3 months ago)

pet store here I come

------------------------------------
Old Donkeys don?t die, they go to:
Drool Donkey Island


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hmmm........


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Offlinedioze1
Satans minion inthe war onlaughter
Registered: 11/23/00
Posts: 384
Loc: Up a hogs ass looking for...
Last seen: 17 years, 9 months
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329161 - 05/29/01 07:54 PM (22 years, 3 months ago)

EXCELLENT stuff, UNA!!
The only question I have is.... Don?t you think 2 hours(not sure of PSI) is a little overkill?
My mother is a retired Microbacteriologist and SWEARS 45 min @15 psi will sterilize ANYthing.
Of course those bags are pretty thick, 2 hours is probably NOT such a bad Idea--it damn sure can?t hurt!
Dioze1

Edited by dioze1 on 05/29/01 09:57 PM.



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Not all that wander are lost.


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
    #329445 - 05/30/01 03:50 AM (22 years, 3 months ago)

Two hours is certainly not overkill. I think it is the minimum time that is required for bags this big.
45 minutes is ok for liquids (maybe even a little bit too long) but for solids such as grain and supplemented woodcips longer sterilisation is required. Depending on the size of the container we sterilise between 1 and 2 hours.
No offense to your mother but 45 minutes will not do the trick

Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.


--------------------
www.911blogger.com


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OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 1 year, 3 months
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329646 - 05/30/01 12:13 PM (22 years, 3 months ago)

Yeah, it?s definitely different for woodchips than anything a bacteriology lab will usually deal with.
I think I remember reading in GGMM something about a recommendation for THREE hours for a certain supplementation of woodchips.
Those pics are phenomenal, just one question. . .

How do you get the moisture content of the chips before puttig them into the bags? Do you just wait until they stop dripping? How long is this on average?

The above information is complete fiction.

I only eat veils


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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/17/99
Posts: 24,162
Loc: my room
Last seen: 12 hours, 57 minutes
Re: this is how we made our azure spawn (pictorial) [Re: Suntzu]
    #329680 - 05/30/01 12:44 PM (22 years, 3 months ago)

This year I didn?t sterilize the woodchips (they were(looked) the same as Una?s)), I only soaked them in warm water for 3 hours, brought to boil, boiled 15 minutes and strained.
NO suplementation, just pure wood chips.
Once cool, innoculated with rye grain spawn.
No contamination problems.

---------------------------------------
kickme.to/mushrooms


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Offlinedioze1
Satans minion inthe war onlaughter
Registered: 11/23/00
Posts: 384
Loc: Up a hogs ass looking for...
Last seen: 17 years, 9 months
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329750 - 05/30/01 01:59 PM (22 years, 3 months ago)

Una, Myself nor my mother are offended:) However 45 min @15 psi will sterilize ANY thing(that we can think of)- I do understand that it would not suffice with your bags of substrate, not because of the substrate it?s self but rather the way that the amount of substrate is being sterilized.
Case and point... if you were to put the same amount of substrate in an autoclave and it was spread out to say an inch or two thick, it would be perfectly sterile in 45 min @ 15 psi.
I have been using rye-grain for about a year now in 1/2 pint jars that are used to colonize other substrates-- I preassure cook them for 45 min @15 psi and have not had any contam problems(while still in the jars) to date.
Anywho... This is a VERY nice post!! Very well documented! If it is not already, you should incorporate this into your site.
Great stuff!! Keep up the good work, it is appreciated.
Dioze1



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