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 Baby_Hitler
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 Do Azures require agar? 
#1298039 - 02/10/03 08:34 PM (22 years, 4 months ago) |
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I'd like to do some Azures. Will I have to grow out mycelium in a petri dish? Somebody told me in another post that you could germinate spores on cardboard. I didn't realize this was possible.
Is it?
Somebody point me towards a good tek. 
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Morality is just aesthetics, meatbags.
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 G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,860
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1298049 - 02/10/03 08:42 PM (22 years, 4 months ago) |
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I dont think you have to start azures on agar.
But if you do i'm about to try cardboard squares on pda + peroxide agar.I think the idear is that the spores germinate on the cardboard(were other spores find it hard to germinate)then the mycelium moves onto the pda + P agar which helps keep contams down.
You can also use cardboard and beer theres a thread here somewere about it.
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er
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mobyd
mad agar worker
Registered: 03/27/02
Posts: 346
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Re: Do Azures require agar? [Re: G a n j a]
#1298572 - 02/11/03 03:45 AM (22 years, 4 months ago) |
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Hi!
I started to isolate spores on Agar. Then transferred onto rye (mixed with some wood chips and cardboard) and then onto wood/or cardboard.
Forget to put agarpieces onto cardboard it won?t work. That?s my opinion.
mobyd
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Sorry, my english is really bad!
If you like my work, rate me....
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Anonymous
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1298738 - 02/11/03 04:55 AM (22 years, 4 months ago) |
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Post deleted by administrator.
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comario2
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Re: Do Azures require agar? [Re: Anonymous]
#1298772 - 02/11/03 05:17 AM (22 years, 4 months ago) |
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yes. as per these pics 
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comario
"crusaders against emotional poverty"
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Baby_Hitler
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Re: Do Azures require agar? [Re: comario2]
#1298938 - 02/11/03 06:21 AM (22 years, 4 months ago) |
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"Yes" you need agar, or "Yes" you can germinate spores on cardboard?
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Morality is just aesthetics, meatbags.
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comario2
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1298955 - 02/11/03 06:26 AM (22 years, 4 months ago) |
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yes to both. the pic i posted is azure myc germinated on MEA. the one posted here below shows spores germination on cardboard placed on a PEROXIDATED agar plate
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comario
"crusaders against emotional poverty"
Edited by comario2 (02/11/03 07:23 AM)
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comario2
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Re: Do Azures require agar? [Re: comario2]
#1298970 - 02/11/03 06:31 AM (22 years, 4 months ago) |
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here is the same petri from the previous post, after the spores have germinated on the cardboard (slowly) and then grown over the - PEROXIDATED - agar (aggressively)
 note that this tek is used to benefit from low contam rate of cardboard (as opposed to non perox MEA) for spores germination. yes, the cardboard i used with success is the thick gray cbd (from the back of note pads)
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comario
"crusaders against emotional poverty"
Edited by comario2 (02/11/03 07:20 AM)
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deanofmean
mycophagous
Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1299086 - 02/11/03 07:03 AM (22 years, 4 months ago) |
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mycelium seems to prefer gray cardboard, like the backing of a notepad .not sure what the difference is .egg cartons work good too .
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Baby_Hitler
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Re: Do Azures require agar? [Re: comario2]
#1299693 - 02/11/03 09:54 AM (22 years, 4 months ago) |
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Do you still have to use a flow hood, or glove box when using cardboard, and peroxidated agar?
Do you have to PC anything?
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Morality is just aesthetics, meatbags.
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Raadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1299740 - 02/11/03 10:11 AM (22 years, 4 months ago) |
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I use peroxidated agar and never have to use a flow hood, or glove box. I just clean up where i'm working.
The process for making peroxidated agar is the same as any agar - mix, and pc - but in the end, you add some peroxide prior to pouring, I use 4cc's in a 500ml mixture.
You will not be able to germinate spores on this agar, but as far as cloning and stuff go, it's great!
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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Baby_Hitler
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Re: Do Azures require agar? [Re: Raadt]
#1300003 - 02/11/03 11:42 AM (22 years, 4 months ago) |
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Do you use the standard 3% H2O2?
Could I buy a jar of presterilized agar, and add peroxide to it?
I won't be using it all the first time, will it keep? Will it have to be refrigerated?
So I pour the dishes, and let them set, then take some cardboard soaked in boiled beer, and stick them in the dish, then streak some spores onto the cardboard?
I think I've almost, but not quite, got it.
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Morality is just aesthetics, meatbags.
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the spiral
Neuroscientist
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1300045 - 02/11/03 12:10 PM (22 years, 4 months ago) |
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great idea
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"A celibate clergy is an especially good idea, because it tends to suppress any hereditary propensity toward fanaticism." - Carl Sagan
Edited by the spiral (02/11/03 12:13 PM)
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Anno
Experimenter
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1300186 - 02/11/03 01:14 PM (22 years, 4 months ago) |
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I?ve been using a different method on various occasions, and it worked well.
I make a fairly diluted spore suspension and inoculate a classic PF cake with it on 4 spots with little inoculant.
One or more of the spots usually show clean growth, then you can transfer those spots to either peroxided agar, or directly to peroxided grain.
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Baby_Hitler
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Re: Do Azures require agar? [Re: the spiral]
#1300211 - 02/11/03 01:25 PM (22 years, 4 months ago) |
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Morality is just aesthetics, meatbags.
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Baby_Hitler
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Re: Do Azures require agar? [Re: Anno]
#1300216 - 02/11/03 01:28 PM (22 years, 4 months ago) |
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Hey, that's pretty smooth!
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Morality is just aesthetics, meatbags.
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Raadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1300237 - 02/11/03 01:36 PM (22 years, 4 months ago) |
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If you buy pre-made agar, yes you can just liquify it in a water bath, then add the peroxide. And if you want to keep the agar for later, you can just recover, and reliquify when ready for more use (you will need to add more peroxide at that point, and your mixture might become a little bit watery (some water on the top of the media) but it should work, even after the 1st, and if not - then you can always just try and be as clean as possible, as you would w/o peroxidated agar. It's not that easy to make a mess of, if you're careful.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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Baby_Hitler
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Re: Do Azures require agar? [Re: Raadt]
#1300261 - 02/11/03 01:45 PM (22 years, 4 months ago) |
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Once I've got the mycelium going, will I have to transfer it to grain, or can I make a big batch of colonized cardboard, and spawn wood chips with that?
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Morality is just aesthetics, meatbags.
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Raadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1300523 - 02/11/03 04:08 PM (22 years, 4 months ago) |
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I would imagine you could go to cardboard, and from there to chips. I believe stamets talks about cyan bed prep that way in one of his books. Cardboard spawning chips, long sheet of colonized cardboard with chips on top.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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comario2
amateur
Registered: 09/06/02
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1301559 - 02/12/03 03:54 AM (22 years, 4 months ago) |
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it would certainly help (to have a flow hood or glove box) but its not absolutely necessary. pc would be necessary for sterilizing grains to be used as spawn
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comario
"crusaders against emotional poverty"
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comario2
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1301565 - 02/12/03 03:57 AM (22 years, 4 months ago) |
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don't use beer, it only increases oppotunities for contams. simply put the cbd in water & microwave for three mins. when cool, place the dot of cbd on the peroxidated agar plate, then streak the cbd with spores
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comario
"crusaders against emotional poverty"
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comario2
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Re: Do Azures require agar? [Re: Raadt]
#1301578 - 02/12/03 04:03 AM (22 years, 4 months ago) |
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do you know of anybody other than stamets reporting success in spawing wood chips only with colonized cbd?
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comario
"crusaders against emotional poverty"
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Baby_Hitler
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Re: Do Azures require agar? [Re: comario2]
#1302044 - 02/12/03 08:06 AM (22 years, 4 months ago) |
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So Azurescens spores will germinate on plain cardboard?
Have you done this yourself?
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Morality is just aesthetics, meatbags.
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Anno
Experimenter
Registered: 06/17/99
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1302056 - 02/12/03 08:11 AM (22 years, 4 months ago) |
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Why don?t you try different methods and see what works for you?
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Baby_Hitler
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Re: Do Azures require agar? [Re: Anno]
#1302080 - 02/12/03 08:22 AM (22 years, 4 months ago) |
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Because I've never done Azures before.
That wouldn't make any more sense than someone who has never done the PF tek trying it for the first time with jars of dog hair, and Moon Pie..
Some limited experimentation is a possibility. Like with beer and without beer. But I need to narrow my parameters as much as possible. There's no point in trying something that's never worked before.
Azure prints are fucking expensive too. I'm about to spend $100 on this project .
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Morality is just aesthetics, meatbags.
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Anno
Experimenter
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1302109 - 02/12/03 08:38 AM (22 years, 4 months ago) |
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But you have done the PF-tek, didn?t you?
I?m just saying that it?s better to start several methods, since the likelihood of suceeding is greater this way.
Woudn?t you agree?
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Baby_Hitler
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Re: Do Azures require agar? [Re: Anno]
#1302200 - 02/12/03 09:13 AM (22 years, 4 months ago) |
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True, I'd like at least 3 variations.
One with small bits of plain cardboard, one with small bits of beer soaked cardboard, and I may try a couple jars of BRF/V.
I'd like to get a few good tubes for slants too. I may not be able to start a patch this year, but I'd at least like to have a good culture that would keep, and be ready to start one next year.
My cultivation options are pretty limited, possibly to whatever I can fit into a small desk drawer.
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Morality is just aesthetics, meatbags.
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comario2
amateur
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1302317 - 02/12/03 09:50 AM (22 years, 4 months ago) |
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yes - same as cubensis, pan cyan, psy cyan, etc etc
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comario
"crusaders against emotional poverty"
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Zen Peddler
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1308382 - 02/14/03 02:03 PM (22 years, 4 months ago) |
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By far the easiest way to grow Azurescens without agar is by spawning wood chips with colonised chips. Its just a matter of adding the colonised chips to moist alder or hardwoods and watching it colonise.
Contaminants are not an issue because generally the wood is selective.
From spores i would suggest agar, but if not, try using a pftek type technique. Make a syringe from the print and innoculate either jars of just striaht woodchips, or a mix of chips, verm and brf to speed things up.
If any of the points germinate and look clean, transfer to peroxidated agar.
Again i will raise the point that just because you are using h202 agar, your still defeating the purpose of sterility if you expose the plates to open air. Contaminant spores will fall on the agar plate and although many may be killed by the h202, some will not and will very much enjoy the conditions when you transfer a wedge to your substrates.
This isnt much of problem with woodlovers, but for mushrooms that require high nutrient substrates contamination is inevitable.
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Baby_Hitler
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Re: Do Azures require agar? [Re: Zen Peddler]
#1308773 - 02/14/03 06:14 PM (22 years, 4 months ago) |
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So what would be the easiest way to get azurescens spawn if you have a peroxidated agar culture?
Check out my ceramic oil lamp I made:
I'm going to try to use it as an alcohol lamp, but the insert was made for oil. I'm not sure how well it will work.
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Morality is just aesthetics, meatbags.
Edited by Baby_Hitler (02/14/03 06:16 PM)
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Anno
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Re: Do Azures require agar? [Re: Baby_Hitler]
#1309012 - 02/14/03 10:44 PM (22 years, 4 months ago) |
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peroxided agar culture => sterilized grain => sterilized/pasteurized chip => outdoor bed with more chips
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