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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
this is how we made our azure spawn (pictorial)
    #328552 - 05/29/01 08:29 AM (21 years, 2 months ago)
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The spawn is based on small woodchips that can be bought in a pet-store. It is used as animal bedding but can also be used to smoke fish and meat. These chips are made from beech.



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:29 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328554 - 05/29/01 08:30 AM (21 years, 2 months ago)

these chips are boiled in water...



Need spores? We got spores!
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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328555 - 05/29/01 08:31 AM (21 years, 2 months ago)
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until they sink...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:31 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328556 - 05/29/01 08:32 AM (21 years, 2 months ago)
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they are drained in a collander...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:32 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328558 - 05/29/01 08:34 AM (21 years, 2 months ago)
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some oatmeal is added to boost mycelial growth..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:35 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328559 - 05/29/01 08:35 AM (21 years, 2 months ago)
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and mixed in....



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:36 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328560 - 05/29/01 08:37 AM (21 years, 2 months ago)
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this mixture is filled into autoclavable spawnbags..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:39 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328563 - 05/29/01 08:39 AM (21 years, 2 months ago)
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these are put in the pressue cooker..
(we put a metal lid on top to prevent the bags from blocking the steam valve)



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:40 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328565 - 05/29/01 08:41 AM (21 years, 2 months ago)
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these bags are sterilised for two hours.



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:41 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328570 - 05/29/01 08:47 AM (21 years, 2 months ago)
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after sterilisation the bags cool down in the flow cabinet..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:48 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328572 - 05/29/01 08:49 AM (21 years, 2 months ago)
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azurescens spawn on rye grain..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:50 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328573 - 05/29/01 08:50 AM (21 years, 2 months ago)
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half of one jar is used to inoculate these bags...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:51 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328574 - 05/29/01 08:52 AM (21 years, 2 months ago)
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After spawning the bags are sealed with an impulse sealer...



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:52 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #328575 - 05/29/01 08:53 AM (21 years, 2 months ago)
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and checked for punctures....after this they are shaken and inubated..



Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/29/01 08:53 AM.



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: this is how we made our azure spawn (pictorial [Re: Una]
    #329020 - 05/29/01 07:54 PM (21 years, 2 months ago)

pet store here I come

------------------------------------
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Drool Donkey Island


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Offlinedioze1
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329161 - 05/29/01 09:54 PM (21 years, 2 months ago)

EXCELLENT stuff, UNA!!
The only question I have is.... Don?t you think 2 hours(not sure of PSI) is a little overkill?
My mother is a retired Microbacteriologist and SWEARS 45 min @15 psi will sterilize ANYthing.
Of course those bags are pretty thick, 2 hours is probably NOT such a bad Idea--it damn sure can?t hurt!
Dioze1

Edited by dioze1 on 05/29/01 09:57 PM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
    #329445 - 05/30/01 05:50 AM (21 years, 2 months ago)

Two hours is certainly not overkill. I think it is the minimum time that is required for bags this big.
45 minutes is ok for liquids (maybe even a little bit too long) but for solids such as grain and supplemented woodcips longer sterilisation is required. Depending on the size of the container we sterilise between 1 and 2 hours.
No offense to your mother but 45 minutes will not do the trick

Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.


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OfflineSuntzu
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329646 - 05/30/01 02:13 PM (21 years, 2 months ago)

Yeah, it?s definitely different for woodchips than anything a bacteriology lab will usually deal with.
I think I remember reading in GGMM something about a recommendation for THREE hours for a certain supplementation of woodchips.
Those pics are phenomenal, just one question. . .

How do you get the moisture content of the chips before puttig them into the bags? Do you just wait until they stop dripping? How long is this on average?

The above information is complete fiction.

I only eat veils


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OfflineAnnoA
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Re: this is how we made our azure spawn (pictorial) [Re: Suntzu]
    #329680 - 05/30/01 02:44 PM (21 years, 2 months ago)

This year I didn?t sterilize the woodchips (they were(looked) the same as Una?s)), I only soaked them in warm water for 3 hours, brought to boil, boiled 15 minutes and strained.
NO suplementation, just pure wood chips.
Once cool, innoculated with rye grain spawn.
No contamination problems.

---------------------------------------
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Offlinedioze1
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329750 - 05/30/01 03:59 PM (21 years, 2 months ago)

Una, Myself nor my mother are offended:) However 45 min @15 psi will sterilize ANY thing(that we can think of)- I do understand that it would not suffice with your bags of substrate, not because of the substrate it?s self but rather the way that the amount of substrate is being sterilized.
Case and point... if you were to put the same amount of substrate in an autoclave and it was spread out to say an inch or two thick, it would be perfectly sterile in 45 min @ 15 psi.
I have been using rye-grain for about a year now in 1/2 pint jars that are used to colonize other substrates-- I preassure cook them for 45 min @15 psi and have not had any contam problems(while still in the jars) to date.
Anywho... This is a VERY nice post!! Very well documented! If it is not already, you should incorporate this into your site.
Great stuff!! Keep up the good work, it is appreciated.
Dioze1



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Offlinesonichell
journeyman
Registered: 11/01/99
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Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
    #329931 - 05/30/01 07:47 PM (21 years, 2 months ago)

a friend has noticed a prob he had with yellow and black mold hitting jars. BUt he thinks it might have been some woodchips he missed that were in the birdseed, anyone else have this prob, either that or airborne or on a scalpel. THis has hit a quite a few jars from the ass end of a bag of penningtons. ANy ideas? anyone have contam from old lids? I soaked the jars in bleach water even.



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OfflineALDER_057
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Registered: 03/14/01
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #329987 - 05/30/01 08:42 PM (21 years, 2 months ago)

Una, nice post, we need to see more posts of this nature. Thanks for the step by step. peace
ALDER



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OfflineDatrutankdogg69
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #330486 - 05/31/01 09:57 AM (21 years, 2 months ago)

Well Done,

Can?t wait 2 see patch pix.

Later,

#P#


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: Datrutankdogg69]
    #330640 - 05/31/01 01:45 PM (21 years, 2 months ago)
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Here are pics of one patch i made last weekend. I also made a Ps. cyanescens and a Ps. bohemica patch.







Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.

Edited by Una on 05/31/01 01:48 PM.



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Offlineholographic mind
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Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
    #331365 - 06/01/01 07:45 AM (21 years, 2 months ago)

hahaha. "my mother is a retired microbiologist and swears 45 minutes are 15 psi will sterilize anything." you are funny. you need to read stamets "growing gourmet and medicinal mushrooms".



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Offlinedioze1
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Re: this is how we made our azure spawn (pictorial) [Re: holographic mind]
    #331469 - 06/01/01 11:40 AM (21 years, 2 months ago)

Call Gerber baby foods and ask who their head Microbacteriologist was for 20 years, I' ll bet yoo 100 dollars the initials will be P.B.
Dioze1
P.S.
Stamets is a pioneer and has contributed greatly to the Mycology community-- But he doesn't know everything and people have been growing mushrooms with out his help or methods for years.

check out the Free Spore Ring!

http://go.to/FreeSporeRing


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Offlineholographic mind
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Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
    #331471 - 06/01/01 11:43 AM (21 years, 2 months ago)

"P.S.
Stamets is a pioneer and has contributed greatly to the Mycology community-- But he doesn't know everything and people have been growing mushrooms with out his help or methods for years."

translation = "I haven't read growing gourmet & medicinal mushrooms. i do not realize that holographic mind is refering to comparative sterilization times"



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: holographic mind]
    #331478 - 06/01/01 11:54 AM (21 years, 2 months ago)

ok...so my thread is turning into a "how long should you sterilise something before it's sterile"...

In think(and i do also know about microbiology and plant tissue culture) the required time depends on:

a) if it are liquids or solids

b) the amount of substrate in a container

Liquids require only brief sterilisation, solids require longer (beacuse liquids conduct heat much better, and don't have small cavities)

The more substrate is in a container, the longer it will take for the heat to penetrate to the core..

I believe that exposure to 121oC for 45 minutes will indeed sterilise anything.....but before the core of the substrate has reached that temperature may take quite some time.

I think you are both right...but you both mean a different thing....

Need spores? We got spores!
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Offlinedioze1
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #331699 - 06/01/01 04:48 PM (21 years, 2 months ago)

VERY well said, Una. 45 min@ 15 psi WILL sterilize anything---BUT it all depends on density and mass---to achieve temps at the core of whatever is being sterilized times NEED to be adjusted.
Una, This is an excellent thread!! I commend you for your efforts to educate the masses and your efforts to save YOUR thread.
Dioze1

check out the Free Spore Ring!

http://go.to/FreeSporeRing


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InvisibleBrownPastures
old hand

Registered: 03/16/01
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Loc: here
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #333447 - 06/03/01 10:14 PM (21 years, 2 months ago)

i dont know what all the huffin' and puffin' is about... it still looks like a hole with a bunch of shit in it.... where's the rest?

"like a Japanese Cowboy or a blind man on skates"Ween
"you must make sure that the lady's pure for the Funky Cold Medina"Tone Loc


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Invisibleslither
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Registered: 02/03/01
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Re: this is how we made our azure spawn (pictorial) [Re: BrownPastures]
    #333479 - 06/03/01 11:06 PM (21 years, 2 months ago)

I think it's one of the best threads that's been on this board in awhile, sure answered alot of questions for me, went in my archives, thanks!!



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Offlinezetek
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Registered: 04/28/01
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #333494 - 06/03/01 11:26 PM (21 years, 2 months ago)

Very nice Una! I learned a lot from your thread. Thanks!



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InvisibleJohn
ssdp.org

Registered: 08/08/03
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Loc: Vancouver, B.C.
Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #333870 - 06/04/01 09:58 AM (21 years, 2 months ago)

Hi,
Don't you think adding oat is really necessary? I think one can get contams if does not work in really sterile environment. You shouldn't put your culture outdoor until spawn is fully colonized also... When you use pure woodchips, you don't have to keep it home and wait till all is colonized...

Edited by john on 06/04/01 10:01 AM.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: ]
    #333885 - 06/04/01 10:24 AM (21 years, 2 months ago)

Don't you think adding oat is really necessary?

No but it speeds up colonisation dramatically. It also produces thicker and more luxurious mycelium. Instead of oatmeal you can also use bran.

I think one can get contams if does not work in really sterile environment.

True, but since we have a laminar flow cabinet (really sterile environment) this is no problem.

You shouldn't put your culture outdoor until spawn is fully colonized also...

No you shouldn't...Of course we let the spawn fully colonise before using it outdoors. Indeed if the woodchips are not supplemented putting them outdoors before 100% colonisation should pose less problems..






Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.


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Offlinecornbread
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #334471 - 06/04/01 10:18 PM (21 years, 2 months ago)

Nice Job Una,
I was curious did you use your wood spawn for a substrate or did you use it to spawn more wood? How deep is your substrate layer?
Thanks



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: cornbread]
    #335033 - 06/05/01 11:10 AM (21 years, 2 months ago)

The spawn was used to spawn more small wood chips. These small chips were soaked for 24 hours. Also bigger pieces of wood (10 x 2 cm) were thrown in (these were soaked for some days).
I estimate that the bed is 25 cm deep.

Need spores? We got spores!
www.mushmush.nl
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Offlinecornbread
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #335183 - 06/05/01 03:05 PM (21 years, 2 months ago)

Thanks alot,
I enjoyed your post.



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OfflineGlacius
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Re: this is how we made our azure spawn (pictorial) [Re: Suntzu]
    #335393 - 06/05/01 07:23 PM (21 years, 2 months ago)

Right on. That was extremely interesting. I am going to have to try it. That was an awsome post. I learned quite a bit from that. Thank's.

Glacius

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OfflineDarK_SavioR
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
    #335604 - 06/05/01 11:11 PM (21 years, 2 months ago)

Awesome, I might try growing some flying saucers sometime soon... you should put this info togethor in a nice little tek guide or something if you haven't already... I'm saving this to my shrooming archive though ; )

"Now chew em up and slam the orange juice. Vitamin C chase, kill the taste. You can tell its nasty by the look on my face."

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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: this is how we made our azure spawn (pictorial) [Re: DarK_SavioR]
    #336336 - 06/06/01 06:24 PM (21 years, 2 months ago)

We added the above information to our website. It can be found on www.mushmush.nl under the "methods" section.

Una

Need spores? We got spores!
www.mushmush.nl
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