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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal] * 1
    #29727206 -

Has anyone experimented with double trenching?

Ive been battling ttbvi with a biofilm. The biofilm actually made it through a cabin. So far ive found that Trenching gives me slightly more time to transfer before the biofilm shows up.

While not pictured in this post, I also have been taking way more aerial transfers. Aerial transfers to trenches have been my jam lately.

Clone to water agar > cabin > 2nd low nute cabin >low nute trench > low nute double trench.











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A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal] * 2
    #29727209 -

Mystikal said:
I'd say more fresh air exchange could be beneficial. I had my lids flipped and slid back creating a big gap while fruiting last flush and it turned out great.

I'm a noob too though.
Welcome to the shroomery.

:popcorn:



Kloakk said:
The coir will get lighter in color as it dries out, it looks fine from here though. After looking at the tub the air exchange should be fine for colonization, but it will take a little longer and you would need to increase some when it's fully colonized I believe.

So 65Β°F and restricted air exchange will slow things down, but shouldn't stall it completely, take a picture every day so you can compare them and determend if it is truly stalled or just slow. If you take off the lid once a day for a picture you'll also change the air, winwin :awesomenod:

You could do as Mystikal recommend, just watch the coir and surface so it doesn't dry out.



Cool thanks all!

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol]
    #29727313 -

Mychol said:
DV8DUG said:
The solution to your problem is an easy fix. Be patient and let it cool down a bit.

Solved.



Just saw your message - I'm using an SAB. Where to put the tool for 1-2 minutes or something where it won't get contam? Sorry if I'm being dense...



Small, easily cleaned rack of some sort where it will rest and flamed part doesn't touch anything. Or how about your hand? you aren't doing anything else....


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29727323 -

I just make my first cut the one that runs parallel to the leading edge. You're not contacting any mycelium so no need to worry about the hot blade, and you're not opening your receiving plate an extra time and jabbing a tool into it an extra time, the blade is cool by the time you go to make the next two cuts of the wedge. Workflow: optimized.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 2
    #29727326 -

Quarter pint jar is my go to:awesomenod::mushroom2:


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29727376 -

Kloakk said:
Mychol said:
Many thanks for your thoughts! I'll forgo the punch unless I can think of a place to cool it without it getting contam, which I can't think of any place at the moment.

And dunking in 99% iso is definitely a no-no to cool it, right?

Thanks again man!

Mike



I can't imagine anything living in 99% iso, so if you flame sterilize it first, maybe, but then you would have to wait while the iso evaporates and you might as well wait for it to cool.

If you really want to use a punch then cool it in the receiving plate and then do the transfer or use a plate just to cool the punch :awesomenod:



:recordscratch: no no no. 99 iso is too strong to sanitize, and even if it could sanitize like 70 does it can combust from a hot tool, and sanitizing is a backwards step from sterilization. Certain things can absolutely resist alcohol, especially stuff so pure. 70% had just enough water to trick cells into absorbing it


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: ambigu]
    #29727386 -

ambigu said:
Question: My new pressure canner just arrived, and I used it to sterilise my reusable polycarbonate plates (Vabiooth). When I took them out of the pressure canner, I noticed that they had warped and become slightly bulged. As a result, they no longer stack properly and keep falling over.
I’ve sterilised these same plates many times in my previous pressure canner without having any issues. I used exactly the same sterilisation method and procedure this time. The only difference is that I haven’t overclocked the weight on my new pressure canner yet, so the pressure was exactly 15 psi instead of about 17 psi.

What could have caused the plates to warp?



It may be a thinner pc.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Mackload]
    #29727392 -

jonkerston said:
Quoting myself from a different thread, bc maybe here is a better place.

jonkerston said:
Cubes, wheat, coco coir. Spawned 2 weeks ago, my tubs are ~13-15l, 1:3 ratio (1l spawn, 3l sub), total sub thickness is ~4cm. My lids don't fully lock, so i've decided to start with lower fae, only have 1 extra 1/4" hole the wide sides.

The mycelium is spreading, but it seems to have stalled colonizing after a while (see pics). The colonized top layer is shiny, the beads are small, maybe perfect but might be a bit on the bigger side - but i don't yet have enough intuition to judge.

I suspect low FAE but not sure. What's the best steps forward?

Sorry that pics quality not best, couldn't make any better.









Now i wonder if surface conditions and air matter at this stage at all, would it just depend on spawn and sub composition?



Mackload said:
looks dry as fuck



100% :whathesaid: Proper Surface Moisture / When to Increase FAE


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29727407 -

Kloakk said:
Mychol said:
Many thanks for your thoughts! I'll forgo the punch unless I can think of a place to cool it without it getting contam, which I can't think of any place at the moment.

And dunking in 99% iso is definitely a no-no to cool it, right?

Thanks again man!

Mike



I can't imagine anything living in 99% iso, so if you flame sterilize it first, maybe, but then you would have to wait while the iso evaporates and you might as well wait for it to cool.

If you really want to use a punch then cool it in the receiving plate and then do the transfer or use a plate just to cool the punch :awesomenod:





Well that was my thought too, to use 99% iso, or 70% since it's supposed to kill spores better, but others have said that's a bad idea. Another buddy said just use it hot, make it a quick punch, and the myc will survive.

I dunno. I might try again.

Thanks for the tips!


--------------------
Mycelium? It's not MYcelium! It's YOURcelium!

Edited by Mychol (11/14/26 07:04 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29727415 -

DV8DUG said:
Mychol said:
DV8DUG said:
The solution to your problem is an easy fix. Be patient and let it cool down a bit.

Solved.



Just saw your message - I'm using an SAB. Where to put the tool for 1-2 minutes or something where it won't get contam? Sorry if I'm being dense...



Small, easily cleaned rack of some sort where it will rest and flamed part doesn't touch anything. Or how about your hand? you aren't doing anything else....



Thank you. I don't care about just holding it - but it would be in my SAB. Stuff can fall on it if I'm waiting for 60 seconds or 2 minutes...unless I had something contam-free to put it in...but what?

Again, sorry to be dense, but how to prevent contam from falling on it while waiting for it to cool?

Or is an SAB supposed to be that good that I can literally hold the tool for 2 minutes and contam won't get on the tool?

Anyways, thanks, and I've enjoyed your posts and learning from you.


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Mycelium? It's not MYcelium! It's YOURcelium!

Edited by Mychol (11/14/26 07:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol] * 1
    #29727418 -

2 minutes might be a stretch but YES.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29727435 -

DV8DUG said:
2 minutes might be a stretch but YES.



Thank you!

I didn't realize an SAB could be that contam-free...I suppose a lot of it comes down to proper technique.


--------------------
Mycelium? It's not MYcelium! It's YOURcelium!

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol]
    #29727440 -

Exactly. Keep the air still and you'll be fine. A stainless steel straw cools pretty quick if you're set on a punch, but it's something I dropped promptly after trying a few times


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29727445 -

tryptkaloids said:
Exactly. Keep the air still and you'll be fine. A stainless steel straw cools pretty quick if you're set on a punch, but it's something I dropped promptly after trying a few times



Yeah...I had dropped it too...but my buddy on this board insists it's super useful. He's a lot better at this than me, so hence all the questions.

I've had some success with just the scalpel...

Thanks for sharing.:awesome:


--------------------
Mycelium? It's not MYcelium! It's YOURcelium!

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29727566 -

What do you mean test it?


--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol]
    #29727652 -

I usually just use a a thick plate of sterile agar to cool blades off as needed, often a small no pour style


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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