SunshineMyco said:

Thanks for the insight! I’ll give the subtropicalis substrate some more thought about removing or lowering the manure content. It seems I may have overestimated how much it needs to thrive. I’ll have a lot of grain spawn so maybe I’ll try a few different methods alongside each other. I appreciate the recommendation.

I would love to be able to test the alkaloid profiles of the strain on different substrates… because in all honesty, the nutrition is why I want manure. Like, we know that adding tryptophan increases alkaloid content because tryptophan is a precursor molecule. I understand that many of the bioactive molecules are at too low of doses to be physiologically relevant, but, analogous to that, just because I can’t outright taste a spice doesn’t mean the spice doesn’t change the dish I’m eating. I believe that all the molecules play a role in the experience, even if 99% of the experience is dictated by a few key molecules.

I also should’ve clarified, the smaller tubs that go inside the 200quart tub will be lidless, because I share your concern about the fog buildup. I figured 200quarts would be the minimum viable size as it can house a terrarium for lizards with controlled humidity and without intense fog & drip build up. The 200quarts tub has its own lid, so it’s just 1 big box with tubs / trays inside. I’ll be sure to post pictures once I have it set up how I want it.

Also great point about the temperature, I don’t think that the two necessarily need to be grown at the same time in the tub, but the hope is that the tub would be able to support both strains through its FAE, humidity control, and tub features. Like for the pans cyans, I can fill up the bottom of the 200qt tub a bit and use an aquarium heater to keep it above 80F.

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Well I don't have any way of measuring potency other then eating the fruits and if you let Subtrops fully mature to the point of near death they are as potent as any shroom and I really don't see why anyone would choose to put all that extra effort into making and pasteurizing a substrate, when you could just eat 0,2g more, but there's nothing wrong with wanting to try things out for science, I have done and keep doing this myself.



Yes HVC will do equally good in a tub as in a automated setup, but in a tub there will be some manual misting and in an automated setup you don't have to do that, plus building a automated setup is fun

And I have grown subtrops, pans, zaps, and paps in my automated tubs so you will be able to do the same, aquarium heater is a common way to grow pans without heating up the whole room, it will work fine.

As long as you smaller tubs/trays makes it easy for fresh air exchange you should be good. Looking forward to see some pictures of your setup

SunshineMyco said:

Thanks for the insight! I’ll give the subtropicalis substrate some more thought about removing or lowering the manure content. It seems I may have overestimated how much it needs to thrive. I’ll have a lot of grain spawn so maybe I’ll try a few different methods alongside each other. I appreciate the recommendation.

I would love to be able to test the alkaloid profiles of the strain on different substrates… because in all honesty, the nutrition is why I want manure. Like, we know that adding tryptophan increases alkaloid content because tryptophan is a precursor molecule. I understand that many of the bioactive molecules are at too low of doses to be physiologically relevant, but, analogous to that, just because I can’t outright taste a spice doesn’t mean the spice doesn’t change the dish I’m eating. I believe that all the molecules play a role in the experience, even if 99% of the experience is dictated by a few key molecules.

I also should’ve clarified, the smaller tubs that go inside the 200quart tub will be lidless, because I share your concern about the fog buildup. I figured 200quarts would be the minimum viable size as it can house a terrarium for lizards with controlled humidity and without intense fog & drip build up. The 200quarts tub has its own lid, so it’s just 1 big box with tubs / trays inside. I’ll be sure to post pictures once I have it set up how I want it.

Also great point about the temperature, I don’t think that the two necessarily need to be grown at the same time in the tub, but the hope is that the tub would be able to support both strains through its FAE, humidity control, and tub features. Like for the pans cyans, I can fill up the bottom of the 200qt tub a bit and use an aquarium heater to keep it above 80F.

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Kloakk is absolutely right and you should actually completely skip the manure with the Subtrops, it's a lot of hassle for nothing. Kloakk uses dreamer tubs and foggers etc and have amazing results but I use simple ez dial tubs and they're doing insanely good in there too. I use nothing but an ez dial tub, millet and coir. As Kloakk mentioned it requires manual misting but this is to show how simple they are and the fact that they don't need much at all please don't overcomplicate Subtrops...

SunshineMyco said:

Sounds good. I’ll keep it simple and go for coco coir for subs for my first go around with them and save the science experiments for after I’ve become proficient in cultivation.

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Or make twice the spawn and do both, then you can dial in the automated tub while you have regular tubs going

PuffM4gicDr4gon said:

Since I don't think anyone else is posted this:

https://www.shroomery.org/forums/showflat.php/Number/29041297#29041297

It's similar to what you're thinking of so you might be able to grab a couple ideas from it

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Kloakk said:

SunshineMyco said:

Sounds good. I’ll keep it simple and go for coco coir for subs for my first go around with them and save the science experiments for after I’ve become proficient in cultivation.

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Or make twice the spawn and do both, then you can dial in the automated tub while you have regular tubs going

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