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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet] * 2
    #29721662 -

probably technique more than the environment


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Learn it, know it, live it


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet] * 3
    #29722094 -

Dj_karma_magnet said:
All of my agar is fucked. I’m on like t3 with 5 plates and they all are bad cobweb contam. Scrapping everything and starting over.


Will air circulation in the house (heat) cause contam? I am working with a flow hood (it’s small, the guy I got it from claims that he just put a new filter in)

The room I am doing this in is messy…grains on the ground from previous grow etc. I am going to vacuum and try to sterilize the fuck out of everything several times before I do this, plan on putting an air purifier In the room several days beforehand.


I will be doing spore needle to agar plate, any tips of tek would help, really really sick of wasting time and money on this


Hey DJKM! I would try to eliminate variables. I'd maybe start with making new agar plates and leaving them open in the flow for a minute, wrap them up and see if they stay clean.
I work in filthy conditions with a SAB. Didn't have problems until the mites found me.
I think I found my mite vector yesterday. We use a clothesline instead of a dryer. My SAB is next to my closet. Yesterday I grabbed some jeans from the closet, found a bird nest in my pocket.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] * 1
    #29722148 -

When I read that the mentioned flowhood is small, my mind went to these small units that some sites are selling, that not necessarily get the best feedback here always. Something about being a fan in box, with no actual laminar flow.

No way of knowing if that might be the case here, but testing or comparing results with a SAB might clear it up a bit. As LadysKnight already recommended.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver] * 2
    #29722253 -

:whathesaid:

You can also use incense to clearly spot irregularities in laminar flow. When I first got my FFU, everything looked fine, but scanning the whole surface with incense revealed a spot near the centre where the smoke was pulled in the opposite direction back towards the incense stick and the filter. That way contaminants from my hands could also be drawn into my Petri dishes. I contacted the seller, and they sent me a replacement filter with consistent laminar flow across the entire surface. It’s working perfectly now.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ambigu] * 5
    #29722290 -

Golden Halo Swab Plate



Yung, a Yeti Iso clone. This cluster was growing from the T0 of the clone sample. I put it into water to try a cluster LI YOLO.



Cloned from the big guy on the left



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 2
    #29722374 -

First time cleaning up LC on agar.


Planning on a mix of regular plates and cabin sequestering for T1.  I feel like I’m racing ahead of bacteria, should I make the transfers today or wait another day or two for the mycelium to grow a bit more?





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Re: AGAR ENVY! (Anything and All things agar!) [Re: Traveler42] * 1
    #29722411 -

:flyby:

get it!


--------------------
Learn it, know it, live it


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mackload] * 3
    #29722481 -

:shiiiiit:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Traveler42] * 2
    #29722893 -

Worst plate work ever. On the 1st I put some spores to agar (and pucks), and a couple of transfers. All mold, including pucks. Mentioned it here- and I think I figured it out.

We were about to meet some friends, wife was showering and getting ready walking back and forth right next to my SAB. I've had so little issues with contam I figured it was fine, where normally I take a break and close things if she's moving around, especially showering. In my tiny place she was moving 20cm next to the SAB and the bathroom is 2.5m away, door open too.



On a positive note, test plates I did trying out the little flow hood are clean 9 days later

:coolpost:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen] * 4
    #29723118 -

Some plates I did 4 days ago. All looking clean, bit of condensation but nothing outrageous.
Have to admit that seeing the plates I poured growing healthy mycelium feels beyond good :rockon:

Ochras. These were agar to agar so... the best looking and fastest ones:




Enigma. LC to agar, particularly excited about these ones:


Flipzilla, again LC to agar:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 2
    #29723216 -

0_tonin said:
Some plates I did 4 days ago. All looking clean, bit of condensation but nothing outrageous.
Have to admit that seeing the plates I poured growing healthy mycelium feels beyond good :rockon:




Good looking plates are the bomb. Faster satisfaction than jars and tubs :headbang:.

I find in the cooler weather condensation is a bitch even pouring a couple of degrees before gelling, and leaving in the SAB for hours with cups of hot water on the stacks. I get condensation on the sides of the plates, which a little bump sends onto the agar, arg!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen]
    #29724275 -

Is it possible for a spore print to become so contaminated from airborne particles that it's effectively useless?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mindexlpora]
    #29724280 -

mindexlpora said:
Is it possible for a spore print to become so contaminated from airborne particles that it's effectively useless?


Yes, never seen one though.


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29724288 -

Kloakk said:
Yes, never seen one though.


Okay thankyou, I will put down my failures to poor SAB work.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mindexlpora]
    #29724306 -

mindexlpora said:
Kloakk said:
Yes, never seen one though.


Okay thankyou, I will put down my failures to poor SAB work.


Post a picture and we'll have a look :awesomenod:


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29724314 -

First attempt at growing psilocybe s. On PDA. Does it look healthy?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Swan Steak]
    #29724331 -

Here's a update on some of the stuff in the works :popcorn:  if any look super super sus lemme know with circles pls xd and the one that has a separate germination wasn't a second germination the gar was more water gar than nute slightly and I tilted it not paying attention n did a quick downward motion when I set it down tossing buncha agar to one side of the plate n also causing my to tear off

















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An enigma some caerulescens n a mono 😎

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Swan Steak]
    #29724350 -

Swan Steak said:
First attempt at growing psilocybe s. On PDA. Does it look healthy?



That looks like pin mold unfortunately.
Why are you opening them?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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