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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 1
    #29723001 -

Tight Lunchbox said:
Send it to a couple grain jars and see how it behaves.


So i did have a couple plates they looked really clean. I sent them to grain and then about 3-7 days after I sent to grain the plates developed the ring.

This is what they look like

These are Pan Cyan TTBVI



Brown rice, and it looks like its eating the rice almost? Not sure ive seen rice look this way.

The original culture can from a clone that came from a bacterial tub. So i know its not 100% clean. But, what gets me is that everything looks really good and healthy,,, then all of a sudden I was up the next morning and there's this dark ring.


mushboy said:
thats one way to find out :lol:

looks gnarly tho i have not seen that.

they cubes or something else? stone myc can do really wacky shit.



TTBVI



Edit: this is what the plate looked like before I sent to grains



This is the grain jar after Inoculation


This is the plate after Inoculation


More colonized jar pre break n shake


The pictures at the top of my response are POST break n shake


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Edited by Lo-Z (11/09/26 02:13 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z] * 1
    #29723022 -

Was about to prepare some coir, so I opened a new 5kg brick (Beats Peat, has been good to me).

I saw some white marks that looked like mold. The block itself does have a bit of a trich smell, like that sharp dusty smell if you know what I mean? Could be a bias because of the pic though.



I flipped it over, and then it looked really weird on side, more like a marking thing.



Havent come across this before over dozens of bricks like this one, but the second pic makes me question. I guess I will email them,.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 2
    #29723026 -

That seems too linear and organized spacing to be naturally forming ? Idk seems like a mechanical marking of some sort.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z]
    #29723065 -

Hey fellas! ive been away for around 6 years and I want to make sure I haven't missed anything important in the monotub world, from what ive been reading monotubs haven't changed a lot, but im seeing people who are putting tubs straight to fruit conditions? And monotubs with small holes not filled, how does that handle contamination rates? Is that updated mono only for stronger isolated genetics that colonize faster?

Thanks!


--------------------
Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

:migraine:

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 1
    #29723076 -

I used Pasty's older school micropore tubs, but recent ones I swapped to his updated Ez Dial, which is yeah, just a few holes. Been working great with my 6qt shoeboxes and 20L tubs.

https://www.shroomery.org/forums/showflat.php/Number/26140339/page/1

It's not that the older methods don't work, they do, but this is a hassle free one, don't have to tape it up, replace tape.

I haven't noticed contamination issues, reality is once its spawned the grain is clean or it isn't, ambient spores aren't going to be the deciding factor.

Only thing that these could be worse with are if you have a gnat problem, since they can enter relatively easily.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 1
    #29723079 -

:whathesaid:

Yep, no distinction between colonization and fruiting conditions. The second the tub is spawned it's in "fruiting conditions"

Variety of cube won't be impacted by the monotub. Follow Pasty's ez dial monotub Tek and you'll be golden :mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge] * 1
    #29723082 -

I'm totally new to agar work. My supplies show up in the next couple days then I'm taking tissue sample and swabs from the albino treasure coast cubensis I have chilling in the fridge.

I have no SAB and plan to do it in open air. I know this isn't recommended.

How likely is contamination doing it like this? Can't I just keep transferring it until I get a clean plate?

Also I'm not planning on growing again for a while, maybe some time in the spring. How long can I keep mycelium on agar?

Any tips for this procedure appreciated.

:cookiemonster:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal]
    #29723091 -

Mycelium on agar in a fridge, even a wedge, will last a long time.

Can you not make a lil sab for it? Hell, even a cardboard box or something?

You will likely get some bacteria/mold if doing it in open air. I will be honest, sometimes for clones I'm lazy and do it, but yeah, then you need to do more cleaning.

Also put it on at least 2 or 3 plates.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29723095 -

Hi πŸ‘‹

Do agar plates need oxygen?

Currently wrapped in electrical tape…. Should I change to micropore or cling film? Is cling film breathable?


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Re: Ask Quick Questions, Get Quick Answers [Re: LaurenceMcKenna28]
    #29723109 -

I have an already sterilized media storage bottle of agar I made like a year and a half ago in my fridge. At one point it froze but it has remained inside the fridge since I made it. Could I still pour plates from it?


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Re: Ask Quick Questions, Get Quick Answers [Re: Quailfishbeach] * 1
    #29723125 -

Quailfishbeach said:
I have an already sterilized media storage bottle of agar I made like a year and a half ago in my fridge. At one point it froze but it has remained inside the fridge since I made it. Could I still pour plates from it?


I would reheat it and remix it in a pot then re PC it so that the agar redistributes evenly.

I had a half quart of leftover solid agar that I didnt use for pouring plates at the time and so i just figured I would save it and re PC it for later...


(I called it Poured and Stored Tek)

So I did just that and when it came out of the PC, the agar was in visible chunks in the liquid mixture (undistributed)

I poured plates with said mixture and the plates turned out with a bit of water that had no agar in the mixture since it was undistributed


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS πŸ“–

Edited by langten69 (11/09/26 04:56 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69]
    #29723130 -

Thanks, you just saved me from doing the same thing πŸ™


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Re: Ask Quick Questions, Get Quick Answers [Re: langten69] * 5
    #29723133 -

So what's the deal? Why is everyone putting questionable spawn and spore to grains? You really are wasting everyone's time. Clean spawn is our objective here... not hoping for just a few top-fruited miracles to grow.



Test it, isolate an axenic culture, then inoculate your grains.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: LaurenceMcKenna28] * 1
    #29723137 -

LaurenceMcKenna28 said:
Hi πŸ‘‹

Do agar plates need oxygen?

Currently wrapped in electrical tape…. Should I change to micropore or cling film? Is cling film breathable?


Plates have enough oxygen in them for the myc to grow a bit

Ive had agar jars stall from no GasExchange (GE) but I opened them up and it resumed colonization

Clingwrap, parafilm, and micropore tape are semipermeable to gases which allow GE

I would switch to one of those


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS πŸ“–

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 2
    #29723140 -

DV8DUG said:
So what's the deal? Why is everyone putting questionable spawn and spore to grains? You really are wasting everyone's time. Clean spawn is our objective here... not hoping for just a few top-fruited miracles to grow.



Test it, isolate an axenic culture, then inoculate your grains.


:whathesaid:

Clean Spawn is King πŸ‘‘


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS πŸ“–

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69] * 1
    #29723159 -



Was going to toss some germ plates that I'd been neglecting for a while, most were moldy, but one had done nothing last I checked, but this is on it now. It sure looks like the right thing to me, but want some second opinions


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z] * 1
    #29723300 -

Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?


--------------------
See no evil, hear no evil πŸ™

Little lady in the southern hemisphere :whiteflag:

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood] * 1
    #29723315 -

The trick is clean cultures, some are a little thinner than others but produce just as well. It's a matter of genetics. No need to heat them, if it's cold in the room either turn the heat up or invest in a oil filled space heater. Temperatures between 65-75 will work just fine. Avoid heating mats. Care to share some photos?

I prefer glass, pyrex is my go to these days as to reduce waste and save on disposables, the clarity is awesome as well. However, when I was starting out, the cell treat 100 x 15mm were my go to. They are convenient for learning sterile technique.

It's mostly preference.

Looking forward to seeing you around:smile::mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29723324 -

DV8DUG said:
So what's the deal? Why is everyone putting questionable spawn and spore to grains? You really are wasting everyone's time. Clean spawn is our objective here... not hoping for just a few top-fruited miracles to grow.



Test it, isolate an axenic culture, then inoculate your grains.


They be limit-testing Dug :awesomenod:

Fonti, my fellow freak, some agar info and time frame would be useful. To my untrained eye it look fine, I'd still make a transfer and see how it behaves. Maybe a cabin for good measure.

CeeGood
:whathesaid:
Mainly personal preference. I'm doing no-pours and I I'm on budget so pp5 containers is my go to.


--------------------
Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: Ask Quick Questions, Get Quick Answers [Re: Reishi Uchiha] * 1
    #29723352 -

Agar is from a vendor lol, I bought a couple swabs and some plates a few months back before starting to do my own pours. P sure its an antibiotic LME plate, streaked it 8.15 according to the label. No idea when growth started, it was an abandoned plate in a stack of contamed ones on the floor lol. Went to toss everything today and noticed this one had woken up and looked kinda good. I had to do some other transfers today so I grabbed a wedge of this for good measure.


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Shop: Myyco.com APE Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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