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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29723001 -

Tight Lunchbox said:
Send it to a couple grain jars and see how it behaves.



So i did have a couple plates they looked really clean. I sent them to grain and then about 3-7 days after I sent to grain the plates developed the ring.

This is what they look like

These are Pan Cyan TTBVI



Brown rice, and it looks like its eating the rice almost? Not sure ive seen rice look this way.

The original culture can from a clone that came from a bacterial tub. So i know its not 100% clean. But, what gets me is that everything looks really good and healthy,,, then all of a sudden I was up the next morning and there's this dark ring.


mushboy said:
thats one way to find out :lol:

looks gnarly tho i have not seen that.

they cubes or something else? stone myc can do really wacky shit.




TTBVI



Edit: this is what the plate looked like before I sent to grains



This is the grain jar after Inoculation


This is the plate after Inoculation


More colonized jar pre break n shake


The pictures at the top of my response are POST break n shake


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Edited by Lo-Z (11/09/26 02:13 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z]
    #29723022 -

Was about to prepare some coir, so I opened a new 5kg brick (Beats Peat, has been good to me).

I saw some white marks that looked like mold. The block itself does have a bit of a trich smell, like that sharp dusty smell if you know what I mean? Could be a bias because of the pic though.



I flipped it over, and then it looked really weird on side, more like a marking thing.



Havent come across this before over dozens of bricks like this one, but the second pic makes me question. I guess I will email them,.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 2
    #29723026 -

That seems too linear and organized spacing to be naturally forming ? Idk seems like a mechanical marking of some sort.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z]
    #29723065 -

Hey fellas! ive been away for around 6 years and I want to make sure I haven't missed anything important in the monotub world, from what ive been reading monotubs haven't changed a lot, but im seeing people who are putting tubs straight to fruit conditions? And monotubs with small holes not filled, how does that handle contamination rates? Is that updated mono only for stronger isolated genetics that colonize faster?

Thanks!


--------------------
Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

:migraine:

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg]
    #29723076 -

I used Pasty's older school micropore tubs, but recent ones I swapped to his updated Ez Dial, which is yeah, just a few holes. Been working great with my 6qt shoeboxes and 20L tubs.

https://www.shroomery.org/forums/showflat.php/Number/26140339/page/1

It's not that the older methods don't work, they do, but this is a hassle free one, don't have to tape it up, replace tape.

I haven't noticed contamination issues, reality is once its spawned the grain is clean or it isn't, ambient spores aren't going to be the deciding factor.

Only thing that these could be worse with are if you have a gnat problem, since they can enter relatively easily.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Twigg]
    #29723079 -

:whathesaid:

Yep, no distinction between colonization and fruiting conditions. The second the tub is spawned it's in "fruiting conditions"

Variety of cube won't be impacted by the monotub. Follow Pasty's ez dial monotub Tek and you'll be golden :mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29723082 -

I'm totally new to agar work. My supplies show up in the next couple days then I'm taking tissue sample and swabs from the albino treasure coast cubensis I have chilling in the fridge.

I have no SAB and plan to do it in open air. I know this isn't recommended.

How likely is contamination doing it like this? Can't I just keep transferring it until I get a clean plate?

Also I'm not planning on growing again for a while, maybe some time in the spring. How long can I keep mycelium on agar?

Any tips for this procedure appreciated.

:cookiemonster:

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