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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29712777 -

Just inoculated 3 agar dishes with GT spores yesterday. The spores are 9 months old. Can I expect growth within a week?

I was thinking about doing PF tek, but saw online that grain can be faster than PF tek but requires a PC? At the same time, people say the mushroom spirit knows when you're rushing it and it will deliberately take longer :hehehe: 
I have a PC now, so I'm considering switching to that and doing a mono tub, but it's only my first time, I'm just super keen to get my first flush, though if mono tubs get a bit of contamination I understand that that means you have to ditch all of it. Is there an in between for Monotubs and say a water tub for grain instead?


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Re: Ask Quick Questions, Get Quick Answers [Re: awsomeguy20211]
    #29712782 -

Shoeboxes, dubtubs, or grow bags.

But what is a water tub? πŸ€” You mean like a sgfc?

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers] * 1
    #29712810 -

PhenoDreamers said:
Shoeboxes, dubtubs, or grow bags.

But what is a water tub? πŸ€” You mean like a sgfc?


https://www.shroomery.org/forums/showflat.php/Number/26601054/vc/1


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: awsomeguy20211]
    #29712839 -

Are you talking about inoculating PF/grain with clean agar or spore syringe?
If it's spore, then PF, grain is not recommended because spores are inherently contamed and contams are likely to grow on grain. You can fuck around and find out, you might get lucky. I had an extra grain jar so I went to fuck around, grain got colonized but it's iffy, not looking the healthiest, gonna spawn since it's just a pint and then find out lol
Definitely not planning on doing it again.

If you don't mind very likely waste, it might be worth as learning experience :2cents:

Since you're eager to get your first flush PF tek is the best bet.
There's something in between but not sure if it's smart to go with MSS: here


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Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29712840 -

Ahh thank you. Are people still using this method for cakes? Personally, I've never done pf Tek or cakes. So I'm clueless in that area

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Re: Ask Quick Questions, Get Quick Answers [Re: awsomeguy20211]
    #29712845 -

awsomeguy20211 said:
Just inoculated 3 agar dishes with GT spores yesterday. The spores are 9 months old. Can I expect growth within a week?

I was thinking about doing PF tek, but saw online that grain can be faster than PF tek but requires a PC? At the same time, people say the mushroom spirit knows when you're rushing it and it will deliberately take longer :hehehe: 
I have a PC now, so I'm considering switching to that and doing a mono tub, but it's only my first time, I'm just super keen to get my first flush, though if mono tubs get a bit of contamination I understand that that means you have to ditch all of it. Is there an in between for Monotubs and say a water tub for grain instead?


Depends on how dry your agar is


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #29713208 -

Absolutely, and as far as I understand it it’s the current convention for fruiting cakes. But not quite my area of expertise either, I just see it recommended by TCs in cake wars and whatnot and what I’ve gathered from compiling teks for the tekdek


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29713241 -

Tekdek you say?:excuseme:


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29713264 -

:feelspokeman: gotta catch em all! Lol


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An enigma some caerulescens n a mono 😎

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Re: Ask Quick Questions, Get Quick Answers [Re: awsomeguy20211] * 2
    #29713280 -

awsomeguy20211 said:
Just inoculated 3 agar dishes with GT spores yesterday. The spores are 9 months old. Can I expect growth within a week?

I was thinking about doing PF tek, but saw online that grain can be faster than PF tek but requires a PC? At the same time, people say the mushroom spirit knows when you're rushing it and it will deliberately take longer :hehehe: 
I have a PC now, so I'm considering switching to that and doing a mono tub, but it's only my first time, I'm just super keen to get my first flush, though if mono tubs get a bit of contamination I understand that that means you have to ditch all of it. Is there an in between for Monotubs and say a water tub for grain instead?


You won't have to throw out monos if you can get clean spawn.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
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Re: Ask Quick Questions, Get Quick Answers [Re: awsomeguy20211] * 1
    #29713331 -

awsomeguy20211 said:
Just inoculated 3 agar dishes with GT spores yesterday. The spores are 9 months old. Can I expect growth within a week?

I was thinking about doing PF tek, but saw online that grain can be faster than PF tek but requires a PC? At the same time, people say the mushroom spirit knows when you're rushing it and it will deliberately take longer :hehehe: 
I have a PC now, so I'm considering switching to that and doing a mono tub, but it's only my first time, I'm just super keen to get my first flush, though if mono tubs get a bit of contamination I understand that that means you have to ditch all of it. Is there an in between for Monotubs and say a water tub for grain instead?


You could get germination and some myc in 9 days, or 30 days. Or 6 weeks. 9 months isn't that old though so I'd expect it faster than slower.

If I'm germinating spores I like to do 2 or 3 plates, and a BRF puck. Sometimes it just doesn't want to get going on agar, or is manky, but on BRF it's a pretty sure bet to get some clean myc to transfer. And if you let it go, you are likely to get some pins, even better.


As for speed- if starting with spores, you can do PF Tek. It can take a month to fully colonize. A few more weeks to fruit. Maybe a bit less, maybe a bit more, depends on the culture, how fast the spores germinate, etc. But it will almost always work. Figure around 6 weeks.

Starting with spores going to grain;
spore to agar or brf puck, germinate (1-4 weeks, likely 1 or 2)
transfer at least once, better 2 or 3 times to get really clean myc
agar to grain, 10-14 days to colonize
spawn to bulk, 15-20 days to pin, a week or so more to mature fruits

So possibly around the same amount of time, probably more, depending on how long each step takes.

With PF Tek you are almost guaranteed some fruits, which can then be cloned, and off you go. With spore to agar/grain/bulk, if you rush anything along the way you could end up dumping a manky tub weeks into it. If you do all the steps correctly, you could take longer than PF Tek, but get more fruits.


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Re: Ask Quick Questions, Get Quick Answers [Re: bluescreen]
    #29713371 -

What happened here…?

So I used the busy man tek and inoculated a millet bag with Natalensis MSS (I have now moved onto agar plates to clean these after having read HHG etc and further educating myself - I understand the MMS was most likely contaminated but I would still like to ask a few questions)

The bag colonising in 3 weeks with very strong rhizomorphic looking mycelium. I then put it in a 33 quart Monotub (pasty’s EZ dialed followed but with micro pore tape as smaller box) and for the first week it was in 22 degrees C/71 degrees F and looking good! Then I left it colonising for 2 more weeks in a fairly cold room (14 degrees Celsius/57 degrees Fahrenheit)  - 3 weeks now in total…

I knew that it was too long but I had to go away for a few weeks unexpectedly after I had made the tub and it was looking so strong!

At first when I returned I saw what just looked like a big patch of blue bruising and a lot of yellow metabolites…. So I decided to take off the micro pore tape as it looked VERY wet and brought it to 22 degrees/71 Fahrenheit for the last few days but now I see (think) (know -  but don’t want to admit it!) that blue patch is turning into the dreaded Trich. 

Where did the contamination come from as I ran another tub with the same substrate mix that didn’t contam. The bag looked so healthy the healthiest I’ve ever grown… was it the severe temp drop trying to pause it? Was it the micopore tape that created too much water inside the tub? Or was it simply the mms… I just don’t understand why it didn’t contam inside the bag initially…

I promise never to go mms to grain again - but yeah any advice on what happened I would appreciate!

Mush love Lauz πŸ’–πŸ„





:crying::crying::crying:


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Re: Ask Quick Questions, Get Quick Answers [Re: LaurenceMcKenna28] * 4
    #29713395 -

LaurenceMcKenna28 said:
What happened here…?

So I used the busy man tek and inoculated a millet bag with Natalensis MSS (I have now moved onto agar plates to clean these after having read HHG etc and further educating myself - I understand the MMS was most likely contaminated but I would still like to ask a few questions)

The bag colonising in 3 weeks with very strong rhizomorphic looking mycelium. I then put it in a 33 quart Monotub (pasty’s EZ dialed followed but with micro pore tape as smaller box) and for the first week it was in 22 degrees C/71 degrees F and looking good! Then I left it colonising for 2 more weeks in a fairly cold room (14 degrees Celsius/57 degrees Fahrenheit)  - 3 weeks now in total…

I knew that it was too long but I had to go away for a few weeks unexpectedly after I had made the tub and it was looking so strong!

At first when I returned I saw what just looked like a big patch of blue bruising and a lot of yellow metabolites…. So I decided to take off the micro pore tape as it looked VERY wet and brought it to 22 degrees/71 Fahrenheit for the last few days but now I see (think) (know -  but don’t want to admit it!) that blue patch is turning into the dreaded Trich. 

Where did the contamination come from as I ran another tub with the same substrate mix that didn’t contam. The bag looked so healthy the healthiest I’ve ever grown… was it the severe temp drop trying to pause it? Was it the micopore tape that created too much water inside the tub? Or was it simply the mms… I just don’t understand why it didn’t contam inside the bag initially…

I promise never to go mms to grain again - but yeah any advice on what happened I would appreciate!

Mush love Lauz πŸ’–πŸ„





:crying::crying::crying:


Not exactly a quick question but the short answer is the mold contam was likely caused by bacteria in the spawn. Bacterial spawn inhibits the mushroom mycelium recovery after spawning and allows mold to get a foothold. Cubes can still colonize a bacterial bag but it slows them down at the critical times when recovery needs to be quick. Often you won’t even see the issue. It takes a practiced eye to spot bacteria in the spawn, to many new people it looks fine, until you know what to look for (growth patterns).


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Re: Ask Quick Questions, Get Quick Answers [Re: Pastywhyte]
    #29713421 -

in other words, grow cubes and cakes folks!

Alao..I love you PW..lets get married or some shit..:loveheart::smug:


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The PF Tek is my area.:oldman:

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Re: Ask Quick Questions, Get Quick Answers [Re: Cakewonder]
    #29714324 -



Happy Helloween

I this normal i see some wet looking RYE Grains is this a bad sign ore just can happen?

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #29714335 -

I usually don't worry until the grains remain wet and the jar is mostly colonized.

Your jar has condensation which could be dripping on top of the grains.

Has the area you have them stored been going through temperature swings? If so, that would explain the condensation


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29714511 -

PuffM4gicDr4gon said:
I usually don't worry until the grains remain wet and the jar is mostly colonized.

Your jar has condensation which could be dripping on top of the grains.

Has the area you have them stored been going through temperature swings? If so, that would explain the condensation






Hey, I do place them above my Heater for Room Temps... Is that not a good think? Temps between 21-23 Celsius

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #29714525 -

Depending on the outside temps, the jars are probably getting cold air from the window and warm air from the heater, leading to condensation.

I would probably move them to somewhere else in the room if they were mine.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #29714526 -

That could be a problem though il let someone give u the definitive answer on that :uhoh:


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An enigma some caerulescens n a mono 😎

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #29714542 -

You'd be better off investing in some garage shelving and storing them atleast 18in away. I used to store jars near a heater, condensation becomes an issue if its too close.

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