langten69 said:

Fiet said:

langten69 said:

When did you 2nd shake?

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week ago ore 10 days

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🤔 im assuming you've seen no growth in 10 days?

Either it needs some more time (Ive had this happen) or it was bacterial and the 2nd shake has spread the bact (Ive also had this happen)

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No, I see growth but after the second shake the Mycelium looks weirdly but time will tell..

This is 10 Day's ago



And then

After few Day's



How Bacteria happen too moisture ore what? I belive i inject too much cc per Jar with 5-6cc per Jar.

Reishi Uchiha said:

I'd like to add to this question. I've inoculated few plates with MSS, some were streaked with loop and some just drops left there. Ones streaked aren't showing any growth yet, while some with only drops started but very slowly. And one with drops, I see black spores on agar but nothing growing. Can it be that agar is a bit dry and that slows it?
I used no-pour, made ~15days ago.
MSS about a month old.
They've been in 68-76 temp.

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Reishi Uchiha said:

I'd like to add to this question. I've inoculated few plates with MSS, some were streaked with loop and some just drops left there. Ones streaked aren't showing any growth yet, while some with only drops started but very slowly. And one with drops, I see black spores on agar but nothing growing. Can it be that agar is a bit dry and that slows it?
I used no-pour, made ~15days ago.
MSS about a month old.
They've been in 68-76 temp.

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Were you the one who made the syringes? Do you still have a print left? Where were you storing your MSS?

I would try 3 things:

1. You can drop a little liquid onto a swab and streak it on the agar — sometimes that helps a lot (or directly from the print).

2. For cases like this, I personally would opt for Pristine BRF Puck, because I’ve already had good results with two varieties in a similar situation. From there, you can transfer it to agar.

3. If you have a way to vortex the syringe, I'd do that if it's been stored for a month, just to homogenize the inoculum. You can make one out of a small motor (I'm not sure if a massage gun works for this).

Agar shouldn't dry out in 15 days.

Hugs

Reishi Uchiha said:

Thanks y'all for help! Sorry for not providing enough info. Streaked plates are a week old and droplet ones are 3 days old.

MSS is from the vendor, stored in dark and coolish place.

I did couple PF tek jars for the cake wars 6 days ago. Can't really tell if there's something growing on them, but one of syringes I sent directly to an extra spawn jar I had around and that one's showing spots of myc.

I have made one syringe with sterilized water to try add few drops to those plates, so I might go ahead and do it. I'm aware spores take longer than LC/clone I just don't know whats the time frame.

Maybe I'm just impatient bastard seeking comfort, in which case I apologize for taking up your time.

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Mackload said:

I'd bet contam the problem more than temps. only way to know if lc is good is try. suggest just many more plates,.more cleaning,.posting more pics before inoculation

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23°C will be fine for colonizing. Put a drop of LC on a plate by pressing the barrel and not the plunger. Leave the drop so you don't spread any contamination around the plate. If and when you see any sign of life you can place your plate with a slight tilt, if there is bacteria the mycelium will have a chance to crawl up the slope while bacteria fall in the other direction.

Baba just recently got his 3 year old LC to come back to life. Post pictures of the plate before sending to grains, pans are not as resilient against bacteria as cubes are so a clean plate is alpha omega.

Reishi Uchiha said:

Thanks y'all for help! Sorry for not providing enough info. Streaked plates are a week old and droplet ones are 3 days old.

MSS is from the vendor, stored in dark and coolish place.

I did couple PF tek jars for the cake wars 6 days ago. Can't really tell if there's something growing on them, but one of syringes I sent directly to an extra spawn jar I had around and that one's showing spots of myc.
Edit: PF jars were noced in the middle.

I have made one syringe with sterilized water to try add few drops to those plates, so I might go ahead and do it. I'm aware spores take longer than LC/clone I just don't know whats the time frame.

Maybe I'm just impatient bastard seeking comfort, in which case I apologize for taking up your time.

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koma23 said:

Reishi Uchiha said:

Thanks y'all for help! Sorry for not providing enough info. Streaked plates are a week old and droplet ones are 3 days old.

MSS is from the vendor, stored in dark and coolish place.

I did couple PF tek jars for the cake wars 6 days ago. Can't really tell if there's something growing on them, but one of syringes I sent directly to an extra spawn jar I had around and that one's showing spots of myc.
Edit: PF jars were noced in the middle.

I have made one syringe with sterilized water to try add few drops to those plates, so I might go ahead and do it. I'm aware spores take longer than LC/clone I just don't know whats the time frame.

Maybe I'm just impatient bastard seeking comfort, in which case I apologize for taking up your time.

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I don't get it, did you buy a spore print or a spore vial/syringe?

You must forcefully hydrate spores if you bought a print.

3 days is nothing, but could start seeing something. A week I'd expect too se germination on properly hydrated spores ...

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DV8DUG said:

Do you use agar at all? Or are you shooting spores into these?

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