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JoeTherapist
Social Scientist— ish

Registered: 05/14/26
Posts: 254
Loc: Snow capped mountain
Last seen: 2 days, 4 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: bloodssonnew]

1
#29698742 - 10/15/26 03:06 PM (9 days, 16 hours ago)

bloodssonnew said:

And the atl7 I have no idea I think it's baccy af or is just a stone thrower because of the browning/orangeing fuckers getting dark like some of the worst bacterial contam I've had on a cube culture though unlike cubes.... Atl7 can naturally have wierd pigmentation from what I've been

Idk if it helps. This was what my ATL7 jars looked like with some small stone growth, I S2B the jars after harvesting stones and had no issues.

ATL7 plates

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*Not a therapist - Do not take advice from me

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fontinalis666
Fellow Freak

Registered: 07/16/26
Posts: 466
Loc: 6 toe county
Last seen: 1 hour, 45 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: auroradawn]

1
#29698753 - 10/15/26 03:23 PM (9 days, 16 hours ago)

auroradawn said:

So I think these two jars are looking okay after break and shake 48hrs ago (the slightly green tint is a reflection of my pepper plant in the glass)?

Thanks as always and in advance for the help!

Lookin great! Sorghum is an s tier grain :rockon:

:rockon:

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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,339
Loc: Over the hills and far away

Last seen: 3 hours, 47 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Blopblop]
#29698768 - 10/15/26 03:46 PM (9 days, 16 hours ago)

Blopblop said:

650 brick + 2 quarts verm+ 4 qt jars of spawn= - 64qt sterilite

I've never done anything this big

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:hippie:

:hippie:

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Take it easy. if it's easy. take it twice :mushroom2:

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Pastywhyte
Say hello to my little friend

Registered: 09/16/12
Posts: 38,855
Loc: Canada

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]

4
#29698772 - 10/15/26 03:49 PM (9 days, 16 hours ago)

callmehippie said:

Blopblop said:

650 brick + 2 quarts verm+ 4 qt jars of spawn= - 64qt sterilite

I've never done anything this big

You should try at least once. Big tubs are neat. Now scaling up to a bathtub might not be a great idea but I have some tips on it should anyone be that nutz.

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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,339
Loc: Over the hills and far away

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Re: Ask Quick Questions, Get Quick Answers

[Re: Pastywhyte]

4
#29698782 - 10/15/26 03:56 PM (9 days, 15 hours ago)

:rockon:

I'm gearing up more towards it.

now that I've got some skin in the game.

Now that I can confidently perform every step of the process without having to ask a single question or second guess myself.

there's no more pressure or anything. Basically it's all fun from here. All downhill. in a good way!

I'm super patient. And I didn't want to waste so much sub all at once while going through my trial and error stage.

This has been my reasoning so far.

I'm not going to lie. A really nice looking canopy on a big mono has a lot of sex appeal. :howyoudoing:

:howyoudoing:

--------------------
:hippie:

:hippie:

:mushroom2:

Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

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Reishi Uchiha
filthy pirate

Registered: 09/29/26
Posts: 87
Loc: 7 seas

Last seen: 8 hours, 31 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Pastywhyte]
#29698784 - 10/15/26 04:01 PM (9 days, 15 hours ago)

Bathtub you say? I have one dysfunctional bathroom. Might call you on that one day mate Team Nutz :rockon:

:rockon:

:crazy2:

:crazyeyes:

--------------------
Some say we'll see Armageddon soon
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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,339
Loc: Over the hills and far away

Last seen: 3 hours, 47 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

2
#29698789 - 10/15/26 04:09 PM (9 days, 15 hours ago)

Pasty’s bathtub grow was epic. :rockon:

:rockon:

I don't remember who did the Kiddy swimming pool.

but that one was cool too.

Definitely going to make use of the one or two I have laying around for some outdoor patches.

--------------------
:hippie:

:hippie:

:mushroom2:

Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

:mushroom2:

:sporedrop:

The Hitchhiker's Guide to the Shroomery

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**A LINK TO EVERTHING**

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langten69
🥋 Contam Eradicator : PE Guru

Registered: 08/18/26
Posts: 1,219
Loc: 'Merica
Last seen: 6 hours, 9 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]

2
#29698887 - 10/15/26 06:05 PM (9 days, 13 hours ago)

Smellyhobbit said:

callmehippie said:

Somtimes I eye ball and count the handfuls.

Half of the time.. I don't measure at all. I just look for good mixture and the sub depth that I'm trying to achieve...

The other half.. Same as the others.

It’s that bell curve meme. On the low IQ end you have “I don’t measure. Just whatever looks right.” In the middle you have precise measurements. Then again on the high IQ end you’ve got “I don’t measure. Just whatever looks right.”

:rollsafe:

--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶*underconstruction*
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
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langten69
🥋 Contam Eradicator : PE Guru

Registered: 08/18/26
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Re: Ask Quick Questions, Get Quick Answers

[Re: Pastywhyte]

1
#29698893 - 10/15/26 06:12 PM (9 days, 13 hours ago)

Pastywhyte said:

callmehippie said:

Blopblop said:

650 brick + 2 quarts verm+ 4 qt jars of spawn= - 64qt sterilite

I've never done anything this big

You should try at least once. Big tubs are neat. Now scaling up to a bathtub might not be a great idea but I have some tips on it should anyone be that nutz.

:rofl:

--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶*underconstruction*
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

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Reishi Uchiha
filthy pirate

Registered: 09/29/26
Posts: 87
Loc: 7 seas

Last seen: 8 hours, 31 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29698896 - 10/15/26 06:16 PM (9 days, 13 hours ago)

QQ: can I spray ISO on hot needle/knife too cool it down or is that possible contam vector?

--------------------
Some say we'll see Armageddon soon
I certainly hope we will

:madpirate:

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Huskies
Boop More Snoots

Registered: 03/22/16
Posts: 2,769
Last seen: 41 minutes, 24 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

1
#29698901 - 10/15/26 06:22 PM (9 days, 13 hours ago)

The flame is the ultimate cleanser, trust it. It needs nothing else after, except to cool down, you can squirt some syringe solution out for that

--------------------
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langten69
🥋 Contam Eradicator : PE Guru

Registered: 08/18/26
Posts: 1,219
Loc: 'Merica
Last seen: 6 hours, 9 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Huskies]
#29698906 - 10/15/26 06:28 PM (9 days, 13 hours ago)

:whathesaid:

--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶*underconstruction*
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,208
Loc:

Last seen: 25 minutes, 42 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]

2
#29698911 - 10/15/26 06:34 PM (9 days, 13 hours ago)

callmehippie said:

Pasty’s bathtub grow was epic.

I don't remember who did the Kiddy swimming pool.

but that one was cool too.

Definitely going to make use of the one or two I have laying around for some outdoor patches.

Hamloaf i think? Id like to see someone dial it in. It got a little soggy in the middle iirc. Probably the rain settled to the center of the plastic sheet over the top

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,561
Loc: Booger Central
Last seen: 9 hours, 15 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

1
#29698913 - 10/15/26 06:38 PM (9 days, 13 hours ago)

Reishi Uchiha said:

QQ: can I spray ISO on hot needle/knife too cool it down or is that possible contam vector?

you don’t even need to cool down

--------------------
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:kodama:

Anyone can get a canopy. But can you get what you want?

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Tri
🎀𝒫𝒶𝓇𝒶𝓂𝑒𝒸𝒾𝓊𝓂 𝐵𝓇𝒶𝒾𝓃🎀

Registered: 08/24/18
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Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

2
#29698932 - 10/15/26 06:56 PM (9 days, 12 hours ago)

Reishi Uchiha said:

QQ: can I spray ISO on hot needle/knife too cool it down or is that possible contam vector?

Isopropyl alcohol isn’t sterile

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MushLuvR77
Mushine Head

Registered: 01/23/25
Posts: 790
Last seen: 8 hours, 52 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]

1
#29698937 - 10/15/26 07:02 PM (9 days, 12 hours ago)

Big grows are what excited me about going for it (shout out to Choempi on RIU, and eventually FrankH) went back-to-back Mono's in 2011, Ralphster spores 7 quarts of WBS (2nd Mono was 6qts 1 jar was slower) yup, we used to shoot MS into grains via flamed needle in open air, the risk made it exciting too, now here I am years later finally doing cakes with MS (started on grain, I know, different path than most)... makes me chuckle every time.

The 66qt tubs have changed over the years, I still have the original, she's a Frankenstein tub, been through it with all my projects. Once the Polyfil finally ran out, took a long time by the way, micropore tape was mush easier and I wasn't using them anyway, switching over to mainly 12qt tubs or 6's later on. Anyway, been a LONG time doin' a Mono... shit probably 2017 or 18, we should break out 4-5 quarts, regular cubes for nostalgic purposes, and goto... WAR!

Newer 66qt

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Reishi Uchiha
filthy pirate

Registered: 09/29/26
Posts: 87
Loc: 7 seas

Last seen: 8 hours, 31 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]
#29699165 - 10/15/26 11:09 PM (9 days, 8 hours ago)

Quote:

Smellyhobbit said:you don’t even need to cool down

That's the other thing I was wondering about, thank you Smelly shireling!
And thank y'all for your input, I'm grateful to be part of this :happiness:

:happiness:

--------------------
Some say we'll see Armageddon soon
I certainly hope we will

:madpirate:

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fiddle_head
sly capuchin

Registered: 08/05/08
Posts: 5,027
Loc: o
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Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

2
#29699170 - 10/15/26 11:12 PM (9 days, 8 hours ago)

Reishi Uchiha said:

QQ: can I spray ISO on hot needle/knife too cool it down or is that possible contam vector?

as others have said no.
flame sterilize only, spraying with iso just cancels that out

--------------------
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hamloaf
Pork Prophet

Registered: 12/23/09
Posts: 29,899
Loc: Greasy forked truth typer

Re: Ask Quick Questions, Get Quick Answers

[Re: Reishi Uchiha]

2
#29699183 - 10/15/26 11:20 PM (9 days, 8 hours ago)

Reishi Uchiha said:

QQ: can I spray ISO on hot needle/knife too cool it down or is that possible contam vector?

If it’s a red hot needle, there’s no need to cool it off. It wil become cool once the inoculant touches it.

If it’s a red hot scalpel, bury and resurrect it quickly into uncolonized agar before cutting into the culture.

It was me who did the kiddy pool. If I were going to attempt that for real, next time I would make the substrate at least a third of the thickness of that last one.

--------------------

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mobyhat
ShroomerNewbie

Registered: 10/09/25
Posts: 252
Loc: Portugal

Last seen: 1 hour, 30 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: hamloaf]
#29699364 - 10/16/26 03:42 AM (9 days, 4 hours ago)

Albino PE agar; have bacteria...how I can isolate this? I make 3 trasfers..but bacteria its in new agars.. 😬 what can I do?

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