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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: tbone8352]
    #29671753 -

All these comments lead me to this idea...

An organism/culture that produces both THC & Psilocybin (or *insert your chosen conpound here*)

Reading all these articles and papers about people doing experiements have made one thing very clear to me

Cultures of different morphology/function simply have no problem in cohabiting with one another

So this gives a lot of freedom to the individual cultivator what sort of cultures they choose to let comingle

Me personally, this leads me to think that one can make a superplant/superculture that is the "best of both worlds" or however many worlds the individual cultivator chooses to put together


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29671873 -

There is no harm in experimenting! When it comes to plants and fungi, they already have a very complex relationship. There actually is some natural horizontal gene transfer between some symbiote fungi and their partner plants, and even with some parasitic fungi.

I bet that would actually be a greater avenue for success then some kind of hybrid project. You still run into the problem of needing the fungus to produce psilocybin in such a way, that it's possible for the plant to make it with the same genes.

But, lichens do exist and many of the are composites of fungi, algae, and yeasts/bacteria.

I would be very interested to see someone try to force a composite organism, one being a psilocybin mushroom, the other a algae or cyanobacteria. Unlikely to work, but would be fun to try.


--------------------
"You develop an instant global consciousness, a people orientation, an intense dissatisfaction with the state of the world, and a compulsion to do something about it. From out there on the moon, international politics look so petty. You want to grab a politician by the scruff of the neck and drag him a quarter of a million miles out and say, 'Look at that, you son of a bitch.'"

โ€” Edgar Mitchell, Apollo 14 astronaut

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: tbone8352]
    #29671952 -

Makes sense enough to me.  Cooperation is a highly advantageous evolutionary strategy, after all.  Get em to work together toward a common goal (survival and/or fucking) and everyone benefits.

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: DorkWithTheHair]
    #29672297 -

Quote:
I would be very interested to see someone try to force a composite organism, one being a psilocybin mushroom, the other a algae or cyanobacteria. Unlikely to work, but would be fun to try.



Creating composites is basically the natural role of mycorrhizal mushrooms, creating conditional alliances when closely living together. The two species will never become just one species, but might be growing together so closely as to make the distinction moot.

For example truffles are composite organisms as I discuss here, they have living within them several bacterias and yeasts especially in the outer rind, these are for the most part beneficial to it, and help it produce its notorious scent. I learned recently they actually have Trichoderma living in them as a passenger, and when truffles decay they release Trich into the soil - it probably helps them have a footing into plants, the mold spores are covered in truffle spores, so when the mold spore invades the plant the truffle comes with it. And both being symbiotic to plants, work together. Both of my Tuber plates got taken over by Trichoderma and it came from the fruit body itself, but in one plate the Tuber mycelium is  growing back over the Trichoderma. I will be able to get a culture from a part of it, with the next transfers I think I can make it so Trichoderma no longer appears or sporulates on the plate (though it would still exist as a passenger).

If one wanted to co-culture, it might get given a big headstart through the culture of a mycorrhizal mushroom. Imagine the inoculation of two mushrooms and Tuber Melanosporum, or a mushroom and an algae and Tuber Melanosporum. The Tuber might act like a mediator that can reduce each individual species' hatred of each other, essentially. The others might recognize as 'self' rather than 'not self'.

This is just a theory, and I haven't tested co-culture of multiple fungi with truffles. But when I inoculated live soil with Tuber, it was more likely to develop algaes, mosses and other mushrooms, compared to uninoculated soil. In a controlled manner, you could get a Tuber pure culture and then inoculate exactly the partner species you want.

If I can isolate the Tuber Aestivum from the Trichoderma I'll do a giveaway sometime soon after a few transfers, to anyone who would be willing to experiment in a manner like this or who would just have any use of it at all.

Edited by CreonAntigone (09/18/26 06:20 PM)

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: CreonAntigone]
    #29672304 -

Understood, I meant more like a lichen a true composite.

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29673396 -

langten69 said:
As far as the plasmids go, i need more research on them. I do recall hearing cultivators using snake venom as a plasmid to break down the barriers of two different cultures which resulted in a cross between the two...


Ok so apparently the OG RR was the one who used rattlesnake venom to create a hybrid

I saw a post where tbone8352 says he did it  Here

I am currently looking for the original RR post...

EDIT:I believe This is it

EDIT: I also believe This thread (The Concept of Individual in Mycelium) is quite pertinent when it comes to transgenetic expression


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

Edited by langten69 (09/20/26 12:16 AM)

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29673401 -

It was a good read if u find it post the link I wouldn't mind reading against I don't gotta search through my 1000s of ss,a of information related to mycology :blah:


--------------------





An enigma some caerulescens n a mono ๐Ÿ˜Ž

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: bloodssonnew]
    #29673409 -

bloodssonnew said:
It was a good read if u find it post the link I wouldn't mind reading against I don't gotta search through my 1000s of ss,a of information related to mycology :blah:


Check my edited comment above


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

Edited by langten69 (09/20/26 12:13 AM)

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29674107 -

langten69 said:
langten69 said:
As far as the plasmids go, i need more research on them. I do recall hearing cultivators using snake venom as a plasmid to break down the barriers of two different cultures which resulted in a cross between the two...


Ok so apparently the OG RR was the one who used rattlesnake venom to create a hybrid

I saw a post where tbone8352 says he did it  Here

I am currently looking for the original RR post...

EDIT:I believe This is it

EDIT: I also believe This thread (The Concept of Individual in Mycelium) is quite pertinent when it comes to transgenetic expression


Good find! I counldn't find it for the life of me! It's been over 10 years since I saw that thread.

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: tbone8352]
    #29674993 -

Following this thread :takingnotes:

I did recently pick up a pocket q-PCR that I'm still working on assay development with. Not sure how robust the system is, but it sure as hell is the only qPCR machine I can afford (~600 usd). I hope to be playing a little bit of mad scientist in the future with transfecting mycelium :mushroom2:

On a positive note I was able to get signal for my PsiK primers in sample 5, on a negative note I did also get signal for my 16S bacterial primers :poop: in sample 6. Everything else seemed to fail, but at least my no sample and no primer controls failed as expected.



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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: Zef]
    #29675579 -

Could you share the link of where you picked up your device...I shall like to join you in the quest of being a mad scientist (minus the mad part)

Im looking to get my results and attribute some concrete numbers along with very decriptive words to elucidate my process/progress to the community

Thx


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29677172 -

Yep I got it from here: https://gaudishop.ch/index.php/product-category/pocketpcr/

There are some limitations, it can only run 8 samples at a time - and it can only use the sybr green channel (so one channel per sample). I do want to generate some more reliable data before I strongly recommend it.

If you're handy and have a 3d printer, you might be able to make one yourself for cheaper using the info here: https://github.com/GaudiLabs/qPocketPCR

I'm currently trying to make a low cost qubit alternative from 3d printing as well, you might be interested. This allows you to quantitate the amount of DNA in a sample - which is useful if you intend to try to compare gene copy levels between samples with qPCR. It has great linearity with the qubit system from thermofisher, but doesn't cost 2 grand!

DIYNAFLUOR: https://github.com/traulab/DIYNAFLUOR
https://www.biorxiv.org/content/10.1101/2024.12.16.626200v1

Ultimately I want to try and measure gene load of psilocybin, perform contamination detection, and maybe one day with the time and resources try and do some gene editing stuff.

Edited by Zef (09/24/26 11:30 AM)

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: Zef]
    #29691771 -

tryptkaloids said:
0_tonin said:
I have a question about clones in agar and sending them to a tub.

I have a lot of JF clones going in agar and thinking ahead as I always do.

Is it viable to send into a tub grain colonized with lets say 2 or 3 different clones of Jack Frost? Clones wouldnยดt share the grain jar/bag, they would start sharing the space when sent to bulk. I assume they wont compete each other, they are siblings!

I could see an issue sending clones of different strains/genetics, but since they are all kinda the same... would it work?


Every culture contains several different full sets of genes (even clones, although it does narrow diversity down)

Some genetics get along fine, share information, resources etc. Others are incompatible and dont get along (for lack of a better term, its a complex topic that makes me scratch my head every time)

They are essentially looking for "self" and non self. When they find something they recognize as self they build a bridge via anastamosis and reproduce asexually.
Ones that get along and share information can actually receive genetics from each other and you'll likely not see a tub any different from a multispore grow.

The ones that dont colaborate become competition.

Chances if that happening with clones from the same initial culture are pretty low, but cubes far enough apart genetically may compete.

Theres something to be said about maturity of culture and its ability to merge, so there may be minimal anastamosis post grain colonization, but idk.

Heres some common multi species grows in Workman's guide to common contaminate mushrooms

You can find a bunch of old threads of people using multiple strains per tub, but ive gotta get ready for work. Use a few different search phrases

https://www.shroomery.org/forums/showflat.php/Number/18872209#18872209


This quote was from the ask Q's get A's thread

I felt it was relevant to the conversation so Im posting it here to keep it in mind for myself

So the whole process of HGT is to convince two cultures (that humans classify as different) that they are one in the same, aka Self

Once they see that they can get along without competition of resources, they will share genetics and become one in the same

I will be on the lookout for methods and ways that this can occur in literature, I'll be getting some books and scanning them for old school ways on this possibility


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29691812 -

In plants, you can achieve HGT by grafting and growing out the graft union via tissue culture. One could, for example, create a cannabis/hops hybrid, though I doubt the chances of it being usable or able to reproduce would be very high.


--------------------
O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: yoosername]
    #29691846 -

yoosername said:
In plants, you can achieve HGT by grafting and growing out the graft union via tissue culture. One could, for example, create a cannabis/hops hybrid, though I doubt the chances of it being usable or able to reproduce would be very high.


I have heard of this, I believe that it will only be viable if it can find another cannabis/hops hybrid ๐Ÿค”

This gets me thinking that either hybrid "species" have limited mating options or an explosion of mating options due to their ability to see "Self" in a greater pool of candidates

For example, if I cross a Cube with a King Trumpet, does the cross now see both cubes and king trumpets as potential mating cultures...


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK ๐Ÿถ
PROJECT H.D. ๐Ÿด
PLASTIC-EATING MYCELIUM ๐Ÿด
TRANSGENETIC EXPRESSION ๐Ÿงช
JOIN CAKE WARS III TODAY ๐Ÿซต
MODERN FRUITING CONDITIONS ๐Ÿ„
REVITALIZE THE CUBENSIS GENEPOOL ๐ŸฅŠ
MY FAVORITE TEKS ๐Ÿ“–

Edited by langten69 (10/09/26 03:56 PM)

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: langten69]
    #29692494 -

A fair question, though if memory serves, cross-species hybrids tend to be sterile, don't they?  Kinda like mules, ya might end up with the extremely rare fertile one, but you'd be more likely to get hit by lightning while winning the lottery...or somethin to that effect.

Though, I do wonder if that could be genetically edited in after the fact.  Like, isolate what genes have flipped the reproductive OFF switch, and toggle em back ON.  Though, the ramifications of that would be about as massive for any other germline edits.

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Re: Transgenetic Expression : Getting Nonactive Mycelium to Become Active Producers [Re: DorkWithTheHair]
    #29692534 -

Cross species hybridization can result in impotency inability to fruit n random mutations n if really insanely lucky it's stable enough to fruit good n evin rarer spore out from my little experience ๐Ÿ˜‚ my first attempt ended with grains fully colonized spawned fully colonized n it went kapoot no fruits no pins :hatsoff:

Some day il get it right :popcorn:


--------------------





An enigma some caerulescens n a mono ๐Ÿ˜Ž

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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