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Contamination control and removal are very important technical aspects of microbiological research. Bacterial contamination is very common in fungal cultures. Currently, the commonly used approach for inhibiting bacteria is antibiotic treatment; however, there are drawbacks to using antibiotics, including incomplete removal, limited antibacterial spectra, tendency toward recontamination, effects to fungal strains, and potential risks to the environment. Therefore, in the present work, we developed a new method for bacterial removal from fungi cultured on solid medium, the Cabin-Sequestering (CS) method, based on the different culture characteristics between fungi and bacteria. First, 3–5 mm round or square holes (the “cabin”) are excavated on a solid medium plate. The fungal strain containing possible bacterial contamination is inoculated into the cabin. The cabin is then covered with a sterilized coverslip, followed by incubation at the appropriate temperature. After 7–10 days of culturing, fungal hyphae grow out along the edge of the coverslip; however, the contaminating bacteria cannot pass through the space formed between the medium and the coverslip and, thus, remain in the cabin. The newly grown fungal hyphae around the coverslip are re-inoculated into fresh culture plates, where they form bacteria-free fungal colonies. The CS method is easy handling, with a short experimental cycle and rare recontamination. When necessary, it can also be used in combination with antibiotics in bacterial removal operations.

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Kloakk said:

Cabins are great for getting rid of bacteria.



We already have a good thread about it though:
A trip to the cabin

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langten69 said:

Kloakk said:

Cabins are great for getting rid of bacteria.



We already have a good thread about it though:
A trip to the cabin

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Just trying to mentally process this image, it looks like you put a contaminated wedge in the middle of a cut out section of agar (cabin?) Then put a glass slide (coverslip?) On top of it and only the mycelium crawls its way onto the clean agar, correct me if im wrong

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langten69 said:

Ive been digging into that thread kloakk linked, on it he posted two pics

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Just curious what that golden ring on the second pic is cuz its not in the first

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langten69 said:

Ah ok, im assuming that its a natural byproduct of the whole process then

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CreonAntigone said:

If the glass slides are too vulnerable to flame sterilize, how would you sterilize them? Could they be put in foil and put through a pc cycle?

The agar sandwich method seems to be an easier implementation of this.

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I haven't tried flame sterilizing the slides, I wrap them in foil inside a PP5 container and run them in the pressure canner when I do agar.



The Tao was talking about coverslips, they are smaller and a lot thinner. Nothing wrong with using agar roof as well, I personally just think it's easier to use slides.