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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29648921 -

its not about "sterile"
thats literally only the tip of your tool
pouring is better because exposure to air will happen regardless, ;while adding another vector such as a syringe or SHIP just means your chances of an endospore or bacteria laden dust particle getting picked up somewhere on the way is greater, and syringes are not good for use with colonized LC imo because of the needle/plunger


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Edited by fiddle_head (08/16/26 04:59 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Pastywhyte]
    #29648922 -

my experience with agar is zero, nothing. never done it. will do it now to test LC cleanliness before inoculating jars.

i will inoculate LC with another LC i have, if it test clean in agar.

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Re: Ask Quick Questions, Get Quick Answers [Re: Chiron098] * 1
    #29648931 -

you should defnitely get some agar under your belt before you try lc man. i really would recommend it youll have a much mroe thorough and implicit understanding of the mechanics inside your head. Half of understanding clean work and contam is envisioning the geometry of it.


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Edited by fiddle_head (08/16/26 05:09 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #29648934 -

is not the tip of the blade picking the agar that is the vector of contamination, is the air inside of the SAB. the bistoury can be sterilized using flame sterilization, and is effectively sterile very easily. the main point is the direct contact with air that is not sterile.

using a syringe, you flame sterilize the needle, what make it effectively sterile. then you pull the liquid inside the syringe, that is sterile from fabric. then you flame sterilize the needle again and inject it inside an sterile grain jar through a SHIP. no contact with the air inside of the SAB, like at all.

when you flame sterilize the needle till red hot, the metal get's close to 600C, this is enough to kill all contams in like 1-3 seconds. even if you let the needle cool down for some seconds, is very possible that the needle is still above 200C that is also enough to kill bacteria in seconds. most homemade SHIP are made with automotive silicone, that withstand something close to 350C before burning, so waiting a couple of seconds is enough, what will keep the needle in the 300-200C temperature and not let any contam live in contact with the needle.

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Re: Ask Quick Questions, Get Quick Answers [Re: Chiron098]
    #29648940 -

Chiron098 said:
is not the tip of the blade picking the agar that is the vector of contamination, is the air inside of the SAB. the bistoury can be sterilized using flame sterilization, and is effectively sterile very easily. the main point is the direct contact with air that is not sterile.

using a syringe, you flame sterilize the needle, what make it effectively sterile. then you pull the liquid inside the syringe, that is sterile from fabric. then you flame sterilize the needle again and inject it inside an sterile grain jar through a SHIP. no contact with the air inside of the SAB, like at all.

when you flame sterilize the needle till red hot, the metal get's close to 600C, this is enough to kill all contams in like 1-3 seconds. even if you let the needle cool down for some seconds, is very possible that the needle is still above 200C that is also enough to kill bacteria in seconds. most homemade SHIP are made with automotive silicone, that withstand something close to 350C before burning, so waiting a couple of seconds is enough, what will keep the needle in the 300-200C temperature and not let any contam live in contact with the needle.


i know what happens, youre failing to understand the main point.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29648943 -

i answered your question. by all means use a syringe and a ship. but dont pretend like you are capable of giving me advice or knowing more than me on something you've never actually carried out.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #29648945 -

i even explained in very simple terms why your method is inferior and more contam prone.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 4
    #29648947 -

I pour LC in front of my awesome flow hood.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 7
    #29648950 -

I pour LC in my SAB. Free pour like a free whore, baby. Gnomesayin? Colloquial parochial, praying to the space needle for flamin hot injection port, riding my SHIP into space. Planes, trains, and inoculated grains.

Does this clear things up?


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29648952 -

I don't think LC being more advanced matters in certain work flows. For example if he's just knocking cakes, it's not actually a huge deal if he's not running agar. I did that for a while before I got my agar running. Honestly, while I hope to be done with cakes, I may have to keep using them until my agar game is on point.

Secondly: syringes are a tool that is literally designed to administer sterile fluids. It's a difficult sell to argue against using a purpose built tool to a tradesman. I understand that I'm somewhat new to this stuff and there is plenty to learn, but I don't know that anyone could successfully convince me to use an assault rifle to make a long range shot, when better tool (marksman rifle) exists for the purpose.


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Mycology is my current hyperfixation
Uncommunicated expectations are just premeditated resentments.

Edited by Mistakes (08/16/26 05:45 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #29648953 -

@chrion let me try to explain this way - you're conflating two different things: whether an individual instrument can be sterilized, and whether introducing that instrument into the transfer chain actually reduces contamination risk.

The tip of the scalpel absolutely can be a contamination vector. The relevant issue isn't that the metal was sterile when I flamed it. The issue is what happens after that point. Once the sterile instrument and exposed material are operating inside the SAB, you're dealing with airborne particulate contamination: dust, skin particles, spores, droplets, and everything else that can settle onto an exposed surface. That's precisely why minimizing exposure and unnecessary manipulation matters. The same principle applies to your syringe.

You're treating the LC as though it exists in a binary state of "sterile" versus "contaminated." Real aseptic work isn't that black and white. Your LC vessel has already been opened and exposed to the working environment during inoculation. The goal afterward is to minimize how many additional opportunities you give environmental contaminants to enter the chain.

If I can transfer material directly from the donor to the intended recipient with a sterile instrument, or no instrument at all, (gravity) then adding another vessel, a syringe, another needle, another penetration point, another handling operation, and another interface doesn't automatically make the procedure safer merely because the needle can be flame sterilized. A sterile needle is still another thing that has to be introduced, manipulated, cooled, positioned, and passed through another interface correctly. That's the distinction I posit you are missing: that the sterility of an individual component does not equal sterility of the entire process

It's like putting a sterile third door into a two-door airlock. Yes, the third door is sterile. That doesn't inherently make the airlock safer. You've simply added another interface that has to be operated correctly. If that additional interface isn't providing a necessary function, it's additional complexity and another opportunity for something to go wrong. I'm not arguing that syringe transfers are inherently impossible or that a needle cannot be made effectively sterile. Obviously it can. I'm arguing against the assumption that "sterile needle = no contamination vector." The contamination-control question is the entire transfer pathway, not the cleanliness of one component considered in isolation.

In aseptic technique, unnecessary handling is generally something you remove, not something you add and then justify because each individual piece can theoretically be sterilized.


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----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

Edited by fiddle_head (08/16/26 05:47 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Chiron098] * 1
    #29648954 -

Brother you have 3 of the growing goats telling you the same thing, I would listen to them.

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29648956 -

Dv8 did you make that flow hood cover, I’ve been wanting to make the same thing but I’m not sure what materials to use.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mortifiied] * 1
    #29648958 -

Id be learning agar first, LC second. Never expand LC with LC, always use an intermediary like agar.

SHIPs will more or less waste your time long term unless you’re a wizard.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29648961 -

Just came here to say, holy FUCK melmac takes a long ass time to pin 😤 maybe I should have let things consolidate more before s2b, oh well. It's doing the thing now thankfully 🤘 incredibly thankful for the wealth of knowledge that can be gleaned going through posts on here.


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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #29648962 -

Smellyhobbit said:
I pour LC in my SAB. Free pour like a free whore, baby. Gnomesayin? Colloquial parochial, praying to the space needle for flamin hot injection port, riding my SHIP into space. Planes, trains, and inoculated grains.

Does this clear things up?


:takingnotes:


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Re: Ask Quick Questions, Get Quick Answers [Re: Mortifiied]
    #29648965 -

Mortifiied said:
Dv8 did you make that flow hood cover, I’ve been wanting to make the same thing but I’m not sure what materials to use.


Yeah that's plexi-glass from hardware store. Getting the 1/4" for the top so it wouldn't bow too quickly and thinner for uprights damn near cost me $250 if I remember right. But its sweet...


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29648967 -

My bad, did I hear someone say SHIP piece?


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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29648969 -

Damn that’s more expensive than I thought it would be, what did you use to connect the plexiglass together?

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29648977 -

Kloakk said:
I'm not sure leaving caps in front of the hood would be a good idea even without a lovely cat like the one you have. Caps would dry out quick and unless you make a single line with the caps whatever is on the caps would just blow around on your foil.

I like using some sort of tote for printing. If I'm printing something that takes time I'll put plastic shot glasses over each cap.






This is exactly what I did. I’m glad using the tub was a good way. Just innoced some BRF with my practice prints. I have been doing swabs for agar but want to participate in cake wars, figured prints are better for making solution. And I want to start giving away some spores. 

Thank you so much! I have seen pictures of other people’s caps on foil in front of the hood, but it was probably just for handling.


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Be nice. You are loved. You deserve happiness. You are not alone.

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Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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