Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
questions about beef supreme's guide and issues I'm having
    #29619801 -

Hi all,  I have followed beef supreme's guide to growing mushrooms but have run into some issues and wondered if anyone had any thoughts and guidance.  I used soft white wheat and cleaned and cooked it slowly as per his instructions.  Dried it in a herb rack and then put it in quart jars with lids that have a injection port and a breathing hole. (see images) Pressure cooked them with tin foil on them for 90 minutes and unfortunately after taking them out I did not remove the foil.  I had four beautiful agar plates with my mycelium ready to go and discovered that the jars had a bunch of moisture in them.  I decided to inoculate 2 jars with the moisture in them as an experiment and the other two I let dry over night until the wheat rolled around in the jars without sticking.  Both, after inoculating developed bacteria or mold.  So, my first question is: Do I need to pressure cook the jars with foil and if so, I'm assuming I should take the foil off after cooking to let the jars breathe?  The second question is about my agar inoculation.  I found that when I put the chucks of agar into the jars, they stuck to the sides of the jars when I agitated them to mix.  Has anyone experienced this?  Any suggestions as to how to avoid this?  Any help would be greatly appreciated. 


Extras: Filter Print Post Top
Re: questions about beef supreme's guide and issues I'm having [Re: RelaxedLife53] * 1
    #29619839 -

Hi Relaxed :wave:

90 minutes at 15 psi should suffice. I leave my jars with the foil still on for days before I inoculate them and haven't had any issue, depending on your filter you might not need the foil, but I prefer to have my foil on until I inoculate.

On the contamination my guess is either your plates wasn't clean or something got in during inoculation, sorry to hear it :sad:

Nothing else to dot then make a new batch though and try again :rockon: Only sure way(as far as I know) to avoid getting the agar stuck to the glass is dropping the wedge in the jar and let it colonize the top while the agar dries out for a week or so.

Glad to see you here and just ask if there's anything :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


Extras: Filter Print Post Top
Re: questions about beef supreme's guide and issues I'm having [Re: Kloakk] * 1
    #29619867 -

Derrayld has a nice method for dropping wedges to jars.

https://www.shroomery.org/forums/showflat.php/Number/28037918#28037918


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: questions about beef supreme's guide and issues I'm having [Re: Kloakk]
    #29619881 -

Do you notice any moisture in your jars after pressure cooking and waiting a few days with the foil on? Beef said that moisture is a real killer for what you're trying to do.

Extras: Filter Print Post Top
Re: questions about beef supreme's guide and issues I'm having [Re: LadysKnight] * 1
    #29619887 -

Thanks!! That's very helpful on getting the agar into the grain!

Extras: Filter Print Post Top
Re: questions about beef supreme's guide and issues I'm having [Re: RelaxedLife53]
    #29619899 -

RelaxedLife53 said:
Do you notice any moisture in your jars after pressure cooking and waiting a few days with the foil on? Beef said that moisture is a real killer for what you're trying to do.


If my grains come out too wet I shake the jar(s) every 12 hour or so until they are dry on the surface again before I inoculate. A sterile jar is a sterile jar, but bacteria moves a lot slower on grains that has a dry surface and spores won't germinate as quickly.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Ask Quick Questions, Get Quick Answers
( 1 2 3 4 ... 3919 3920 )		 Nichrome 1,095,316 78,391 08/19/26 08:20 AM
by Kloakk
* Beefs Beefy Tub Tek
( 1 2 3 4 5 6 7 8 all )		 BeefExtreme 17,403 153 07/05/26 11:05 PM
by FuN bAgZzZ
* Busy Mans Guide Contam problems Dogtown1975 194 9 07/11/26 10:29 AM
by Agar Wedge
* Beginner Questions. (help) :) oO_wombat_Oo 995 4 03/16/02 12:56 AM
by Zen Peddler
* A question for Ryche Hawk Jingles 735 2 11/17/01 01:40 PM
by psilocybinjunkie
* small question regarding cake preparation Tim2133 470 2 06/25/03 07:24 PM
by Ekstaza
* Newbie Questions - Help Me Out! :)
( 1 2 all )		 The_Gnome_King 5,436 23 06/04/01 06:51 PM
by Beatnik
* Re: Shroom Wizard's Grow Guide -- Question.. BoomerZ 2,171 12 05/16/00 09:31 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
136 topic views. 13 members, 741 guests and 69 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.012 seconds on 16 queries.