tree frog said:

And this plate has one promising looking culture on it and two the don't look great.  Because the cultures are right next to each other, it makes observing bacteria and other sus growth easier.

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Just to be on the same page on the first photo in my quote, is what you're after the section where they have mated? And if so is this because it shows their compatibility?

And in the 2nd image its the left culture that looks the best? just to verify my guess

Is this also a good way to get a good culture from a print even if im not doing the grab and drag for monos and mating?? Just to get a bit more genetic diversity competing early?

RogerRabbit said:

BPJohnny said:

The Mushroom Cultivator

"Two dikaryotik mycelial networks can also gro together, exchange genetic material and form a new strain. Such an encounter, where two hyphal systems fuse, is known as anastomosis. When two incompatible colonies of mycelia meet, a zone of inhibited growth frequently forms. On agar media, this zone of incompatibility is visible to the unaided eye."

Now correct me if I'm wrong but in ANY multi spore inoculation

there are many different strains so this process will happen

regardless if you mix different syringes or just use one.

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To a certain extent, yes.  They may fuse, and they may remain separate and fruit separate.  When unlike mycelium are placed together on a petri dish, this can be observed.  As Paul said, a line of inhibited growth forms between the two strains.  This can be observed below.  Both of these growths originated from the same sporeprint, but were isolated down to single sector isolated strains.  These two isolates are not compatible, therefore a line of separation has formed between them.


Other times, after the zone of isolation has formed, a distinct third growth will emerge from it. This third growth as shown below emerging from the 12 o'clock position is a hybrid of the two mycelium, and is usually more vigorous than either parent.


When spores germinate and then pair up with compatable hyphae to form multiple strains that then fuse by anastomosis, the results can be seen in the picture below.  The single sector at the bottom of the growth is steadly becoming more of the total as it assimilates the other strains into its network.  If allowed to grow, it might have consolidated the entire dish into one strain or it may have ran up against an incompatible strain as seen in the pics above and stopped.

Holding a petri dish up to light and looking at it from the agar side is a good way to see the line of inihibition between the strains.
RR

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Fuck or Fight isn't a new tek

Quote:

unhinge9315 said:.

I'm going to keep them closed up for a couple of weeks to see how clean they stay. This is day 3 and so far so good!!!

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ModularMind said:

Maybe nocc up a few in the meantime? Couldn’t hurt. That’s what agar’s for. Maybe lick one also.

Congrats on your cake germinations!

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unhinge9315 said:

I made my first attempt at agar the other day.

I used Bod's agar tek found via the HHG.

10 plates, about 25ml per plate using MEA recipe.

Poured inside SAB and then wrapped in cling wrap (figured it wouldn't hurt right?).

I'm going to keep them closed up for a couple of weeks to see how clean they stay. This is day 3 and so far so good!





All the plates look clean close up. Any other artifacts in the pics are just photo shenanigans. Pretty happy!!

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Blizzloo said:

Also,
Does anyone know what's going on here? It's a mush tissue

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Tormato said:

Ha! Finally got some spores to germinate!

Special thank you to Phaethon for the prints! You're a life saver!

Made T1 transfers today. Yeah boi!!      

 

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Nokumana said:

Blizzloo said:

Also,
Does anyone know what's going on here? It's a mush tissue

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I'm not sure what you mean by "going on" but if this is a tissue culture, it looks to me like you've got some reasonable mycillium growing there. 😎

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