Franquiter said:

Why my Yeti isn´t expanding on petri plate?

I transferred from WA to PDA, i did 3 plates, 2 contaminated 1 clean, but the clean one isn´t expanding.

The transfer date was 01/mar.

PD: The small dots, marks, or stains are from outside the petri dish; inside, the agar looks excellent.

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Give it another week mate, the fact it hasn't exploded with bacterial shit means it should be clean. Make sure it's wrapped and kept between 72-76 if you want best results. I've had something not moved for 7-8 days after transfer and then explode.

really is an art unto itself, you'll get to know each of your cultures and how they behave - if you pay attention. I'm only a few months in and I consider every culture an individual soul with their own likes/dislikes, appearances, and growth patterns.

Good luck, god bless, dont give up!

Lithop said:

@Peleus

Splittinghairs and Phaethon are both right haha, I HATE trying to judge plates online. You've got some nice even growth, but on photo it can be tough to impossible to judg the organisation of the leading edge.  What can be simply be faint, new myc can look on photo as a greasy halo of a ride along/competitor of some sort. And vice versa...

That is one of your biggest clues, the edge and its level of organisation.
Although the community can help, theres no substitute for observation and getting to know your own cultures IMO.

Taking a T3 but keeping the donor for observation is good here I reckon, if for nothing more than education.
I like having a little jewellers loup around for my mushroom stuff, it can be handy to study your plates edge with too.

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Lithop said:

But I'm also very fond of using nice murky grainboil water agar.

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