Phaethon said:

Sorry, going to have to duck out for a few hours after this post.

I guess I'm still not sure what issue you were having. We're talking about cubensis? How much were you getting per quart jar and how much are you expecting or hoping for?

I've never used automated stuff for cubes, only gourmets, but aside from slightly less babysitting (shoeboxes in arid climate require more misting than monotubs, which even here I could basically leave alone, but I actually prefer smaller batches), I don't think they'd do much for cubensis. Dialing in conditions isn't too bad if you use Pasty's EZ dial hole patterns and some micropore tape on your tubs.

Do you run clone cultures, and save the cultures that perform well?

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Kunta Kente said:

FamiliarFinding384 said:

With that in mind, what would you preferred method be?

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20 jars of cubes even fruiting subpar should be a decent harvest.

Parroting phaethon: you haven't said what you are growing nor what your issue even was, other than it's not contamination.

If you are getting decent growth on all phases the remaining factors are likely growing conditions or culture work.

Those are assumptions though.

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FamiliarFinding384 said:

Phaethon said:

Sorry, going to have to duck out for a few hours after this post.

I guess I'm still not sure what issue you were having. We're talking about cubensis? How much were you getting per quart jar and how much are you expecting or hoping for?

I've never used automated stuff for cubes, only gourmets, but aside from slightly less babysitting (shoeboxes in arid climate require more misting than monotubs, which even here I could basically leave alone, but I actually prefer smaller batches), I don't think they'd do much for cubensis. Dialing in conditions isn't too bad if you use Pasty's EZ dial hole patterns and some micropore tape on your tubs.

Do you run clone cultures, and save the cultures that perform well?

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Mostly cubensis, but also P. natalensis. I would get a few thick patches in my tubs, but never a canopy. Never ever ever ever, not even close. Yes, I'm certain this is a technique issue. Maybe not huge issues, but it does seem clear that I'm doing things wrong, haha. I haven't covered my holes with micropore - is that your recommendation on all of them in this climate?

I have a few clone cultures, but for the most part, it's been liquid culture from reputable sources (feel free to DM for specifics) on agar. I usually propagate onto new plates every 6 months or so (if not more frequently), so that I've got plenty of fresh and healthy mycelium on plates. Again, I do get contamination from time to time, but it doesn't seem to be consistent and I don't believe it's really the root of my problem. *prays to the contamination gods...* "don't strike me down for my arrogance"

Kunta Kente said:

FamiliarFinding384 said:

With that in mind, what would you preferred method be?

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20 jars of cubes even fruiting subpar should be a decent harvest.

Parroting phaethon: you haven't said what you are growing nor what your issue even was, other than it's not contamination.

If you are getting decent growth on all phases the remaining factors are likely growing conditions or culture work.

Those are assumptions though.

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Yeah - if I did all 20 jars, I'd certainly have plenty - I am hoping for full or majority canopies. I am not sure I've ever gotten a full oz dried from a beefy tub (I usually do 2 quarts per tub - 4:2 coir:grains + 2 quart psuedo case on top), and it seems like that is pretty dang low.

Thanks for your input!

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Archespore said:

It is growing like that because of verticilum?





Can you see any on the rest of the tub?

I already pulled some tissue samples.and I'm growing it out, I'll throw it away if it's contaminated but idk what I'm looking for. I tried looking up verticilum and it doesn't look like anything I can see

Also does that affect their potency?

Thanks

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The rest of the tub looks fine. That's why I covered my ass and said likely

Sorry didn't mean to freak you out. Vert is a soil born invader fungus that can vegetate inside fruits. It's symptoms are typically exactly what we're seeing in the questionable fruit. Malformed caps that tend to be small and stem deformation like blow out. It's super common in the hobby and most are going to see it eventually if you haven't already.
Normally it affects more than one fruit but it's totally possible for that to happen in one fruit if it picked up a spore during growth of the fruit.
It's also possible the fruit took on some damage punching through the casing layer or squeezing out along a tub wall.

Or.. there's some viral damage. That opens up a totally new can of worms lol. Just look at Enigma. Likely a result of viral RNA damage and why it's clonable.

Point being I doubt it's random mutation. Most mutations have a cause. Even science is leaning towards random mutations not being so random after all. So I tend to look for the explainable first.

Go ahead and run those clones out. It may shed some light and certainly gives you practice at the art. I just wouldn't rely on them or even spores from the grow as back up until we see what the results are. If they come out normal then it was probably just damaged while growing. I suppose growing out some of the spores would at least remove a few possibilities too if the results are normal lol.

To answer the last question.. yes stress.. to a point, tends to generate more potency. Subtropicalis is a prime example and we've even measured the difference here with test kits.

And yes.. saying it's just genetics drives me absolutely nuts.