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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33]
    #29493547 -

It's hard to detect fam.  You should be good after 24 hours but if you're worried about it 48.  But you might get a better answer in like the chemistry forum or psychedelic experience.  That said they rarely test for psilocybin.  Are you expecting a test for it?

Also PGT does the oven door thing here at the end for anyone curious.  And yeah he's pouring at 150Β°, which is pasteurization temperature.  Then he's covering them right afterwards. And if you actually look at his hand placement, he would be dropping spores into the cups even if the up draft from the oven worked.



It's an open air pour lol  And I bet those plates have as much condensation as they would if he had just taught folks to no pour.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 2
    #29493550 -

Pickyourpoison33 said:
tree frog said:
I don't know what the point of the oven was other than to give bad advice and generate content :shrug:


Not saying this will work or anything because I haven’t ever touched agar yet you all probably know a lot better than me but I think the concept is being looked at as similar to a Bunsen burner is why people are spreading that information around


Just curious as to your opinion on detection timeframes for psilocybin on LC/MS//MS urine testing im curious because of how unstable psilocybin is due to oxidation





Psilocin, the active metabolite of psilocybin is detectable in urine for a short duration, typically up to 24 hours after use, though it may be present for up to 48 hours to 3 days in some individuals depending on metabolism and dose.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29493642 -

The guy on Fungaia who seemingly does good work has a good YouTube video on sterile techniques. I gather that old school folks could do sterile work next to an alcohol lamp - the heat plume of the flame would create a small but workable "sterile bubble" around your work and if you were patient, careful, and lucky, you could do a fair amount. That was perhaps the gold standard way back when, and a strile laminar flow hood is the gold standard now.

I don't see a SAB as a shortcut. It's tried and true. A proven technique with repeatable results - That's pretty much the definition of solid scientific evidence. It's even peer-reviewed.

I can't speak to whether or not a convection oven makes a good laminal flow device but seeing as you can't filter the air that's getting blown all over your work I'll hazard a guess that you're asking for trouble - unless, of course, you want to get some practice in on cleaning up contaminated plates. That's actually a worthy goal and something that I spent a lot of time doing in the first few weeks that I started Agar work, and those skills continue to come in handy. - and yeah yeah I'm like the LEAST experienced cultivator here, but I've done a lot of sterile work in other aspects of my life and career So I'ma take the liberty of expounding here this time. πŸ™

Peace
:peacesign:


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #29493733 -

PhenoDreamers said:
Anyone get this error message when trying to upload pictures before?

"1000006475.jpg was bigger than the allowed file size of 5120 KB and was not saved."


I used to get that before I was a paid member here.

I have had errors uploading since becoming a paid member that were fixed simply by refreshing the page and trying again.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29493772 -

PhenoDreamers said:
Anyone get this error message when trying to upload pictures before?

"1000006475.jpg was bigger than the allowed file size of 5120 KB and was not saved."



For iPhone: when uploading image select the image and there will be 3 dots on the bottom left of the screen. Then select option. Choose medium or large for size (just make sure the file size is under the limit).




Edited by NotImlay (02/22/26 06:44 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 3
    #29493820 -

Pickyourpoison33 said:
Sealifefungi said:
Laminar flow with the oven ?? I tried searching g could not find anything . I need to transfer some spores to an agar dish , everything is already poured and sealed . I have a STAB the honey got off amazon ( I know I know ) I haven’t set it up yet it’s huge . And don’t wanna set it up for one transfer . I can obtain laminar flow by running oven at 250 cracking door and placing a tray there ? True or false


I saw a video of PGT (philly golden teacher) doing that with pouring his agar plates the other day on youtube so im thinking that should work you can look it up and see what you think


That guy is an ass and pushes awful misinformation.  I would not ever take his advice on anything.


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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 4
    #29493831 -

Pickyourpoison33 said:
Phaethon said:
Shortcuts do not pay off in this hobby, unless you like dealing with contaminated cultures and pathetic harvests.



I agree wouldn’t still air box also be considered as a shortcut tho technically


Still air boxes are used in labs around the world to do clean work.  They are dedicated tools designed for one purpose, to stop air currents from moving particles around, thereby allowing you to do clean work as nothing will fall into your open plates or jars.

An oven is an open box with heat convection currents that you bake muffins and chickens in.

People want to use an oven because the completely misunderstand what a still air box is and how it functions.  It’s not clean or even often sanitary in a still air box, there are just no air currents to carry contamination onto your work.  They think by getting the oven real hot it will kill any contamination present, but that’s negated the second you open the door and whoosh air around. 

My eight dollar still air box works better than most contraptions sold on the internet for clean work, be they fake flow hoods or expensive soft sided glove boxes.  It works a hell of a lot better than an oven.  What is it?  It’s a cheap tub with large arm holes.



--------------------
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29493842 -

Thanks. I have read many guides here and got a bunch of TEK's mixed up in my head. Maybe where I got the idea of using a indoor grow tent as some kind of all in one device for SAB and FC. The humidity here is about 30% but gets up around 70% in the green house with a wet puppy pad. I kept the grow 65 qt. grow bin in it ti try and help prevent it from drying out too fast. Now I am working on PF-TEK trying to KISS.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 3
    #29493844 -

Pickyourpoison33 said:
tree frog said:
I don't know what the point of the oven was other than to give bad advice and generate content :shrug:


Not saying this will work or anything because I haven’t ever touched agar yet you all probably know a lot better than me but I think the concept is being looked at as similar to a Bunsen burner is why people are spreading that information around


Just curious as to your opinion on detection timeframes for psilocybin on LC/MS//MS urine testing im curious because of how unstable psilocybin is due to oxidation


If you’re getting randomly tested for drugs, the only safe option is to not do drugs.

Most drug tests do not include psilocybin, but that doesn’t guarantee yours won’t.


--------------------
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 4
    #29493985 -

Pickyourpoison33 said:
tree frog said:
I don't know what the point of the oven was other than to give bad advice and generate content :shrug:


Not saying this will work or anything because I haven’t ever touched agar yet you all probably know a lot better than me but I think the concept is being looked at as similar to a Bunsen burner is why people are spreading that information around


Just curious as to your opinion on detection timeframes for psilocybin on LC/MS//MS urine testing im curious because of how unstable psilocybin is due to oxidation



It's nice that you're trying to help folks out but when you say stuff like "I saw a video of xxx doing yyy so I think it will work" you're spreading bad practices around. If you have zero experience with cultivation, you should be giving zero advice in my opinion. You're doing more harm than good. That chap needed to be told "go buy a $10 tote and make an SAB" you know?

Anyways on to your main reason for posting here:

Are you worried about popping on a piss test because the DOD added psilocin to its drug panel recently? The detection time-frames for these metabolites are well documented in studies so I'm assuming you're asking because you're being screened but still want to eat mushrooms...

The way they screen is by immunoassay which has a much higher threshold for detection. Say 500ng/ml. Then they take all the preliminary positives and run those through the much more expensive (and accurate) LC/GC-MS with a threshold closer to let's say, 5ng/ml. This proves (as long as the custody chain remains unbroken) that the person who pissed in the cup used drugs.

As advised the best practice would be abstinence, if you dont want to get fired.

If you're willing to accept the risk of a non-zero chance of termination...

Next best thing would be to do your drugs on long weekends / holiday / vacation to maximize your time between consumption and screening, while also using dilution, substitution, and deceit as backups.

Generally speaking if you're not a fattie and you take shrooms Friday night you'll be pissing clean by Tuesday morning.

I successfully navigated random and regular federal piss tests for nearly a decade at my last job. If you'd like I can share my experiences in another thread or directly with you through private message.

A multi-faceted approach with several layers of redundancies was the only way I was willing to accept the risk, at the time.

Take care


--------------------
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- New here? Me too! Pop by and say hi c:

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Re: Ask Quick Questions, Get Quick Answers [Re: Archespore]
    #29494338 -

How much does a brick of coir cost where y'all are at? I'm paying $3.80 a brick for coco bliss right now. (650g)


--------------------
The Hitch Hiker's Guide

Bod's TEKs

Pastywhyte's Easy Writeups

Baba's TEKs (Exotics and more)

callmehippie's Extensive List of Everything

Anything and Everything Else You Will Ever Need

Edited by RhyzoPhreak (02/23/26 06:32 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: RhyzoPhreak]
    #29494346 -

Just got 5kg (make 75ltr) for equiv $19, in uk. (14 quid)

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Re: Ask Quick Questions, Get Quick Answers [Re: Lithop] * 2
    #29494356 -

RhyzoPhreak said:
How much does a brick of coir cost where y'all are at? I'm paying $3.80 a brick for coco bliss right now. (650g)


I pay $4.10 for a 700g brick of Jiffy Pro 7.

Lithop said:
Just got 5kg (make 75ltr) for equiv $19, in uk. (14 quid)


We're neighbors :eek:

:nice:


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29494360 -

:yesnod:
Why do you think I'm so selective about the type of stuff I say to you?
:rollsafe:
:high5miss:

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29494364 -

Cries in Canadian





I couldn't find it this time, but I've seen 10 packs of coco bliss for $15 a brick on amazon

You can get no name brands for quite a bit cheaper, but the last time I tried that I had multiple tubs that refused to fruit.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (02/23/26 07:19 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29494365 -

:waitwhatthehell:
$221.73 seems...... WHat the fuck?

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Re: Ask Quick Questions, Get Quick Answers [Re: Lithop]
    #29494369 -

It was out of stock too, which means people bought it.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29494382 -

:lol:
How could they pass it up?
Imagine the water youd want to be hydrating that with. Primo shit

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Re: Ask Quick Questions, Get Quick Answers [Re: Lithop]
    #29494384 -

The really cheap coir smells like diesel fuel.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29494392 -

I've definitely had a few bricks of fuelish and/or plasticy smelling coir.

I'm pleased my big brick has a neutral scent or it'd be a lot of bad coir to use.
:racethread:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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