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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Sockadin] * 2
    #29493317 -

It's pretty new generally.  A popular FB cultivator has been experimenting with it.  All stuff I've been hearing second hand as I don't go on there.  But I have talked to the guy whose doing it and followed some of his YouTube stuff on breeding, before Google killed his account.

Anyway, the pee pads or diapers, etc.  They're all plastic basically.  So it's a mold and bacteria resistant moisture reservoir.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: towndaze]
    #29493319 -

towndaze said:
I have access to a heavy duty juicer. Can I just juice potatoes, filter the results and use that as LC base?


Now avoiding twice cooked potatoes would be interesting. Might have some clarity issues in LC but a side by side comparison to the standard PDA agar recipes is immediately where my brain went lol. Might have to try it on the next round.

Thanks!
:manofapproval:

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo] * 1
    #29493322 -

I'd suggest reading this thread I linked a bit ago before spending money.

https://www.shroomery.org/forums/showflat.php/Number/23990888/vc/1


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29493332 -

tree frog said:
I'd suggest reading this thread I linked a bit ago before spending money.

https://www.shroomery.org/forums/showflat.php/Number/23990888/vc/1


BIG plus one for this ☝️☝️☝️


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Sealifefungi] * 3
    #29493353 -

Sealifefungi said:
Laminar flow with the oven ?? I tried searching g could not find anything . I need to transfer some spores to an agar dish , everything is already poured and sealed . I have a STAB the honey got off amazon ( I know I know ) I haven’t set it up yet it’s huge . And don’t wanna set it up for one transfer . I can obtain laminar flow by running oven at 250 cracking door and placing a tray there ? True or false


Like frog said, 100% false.  Just make a SAB out of a rigid walled cheap tub and be done.  Try framing not using the one your partner got you as “we’re learning together” instead of “You got the wrong thing.”

Here’s an example of a super cheap SAB that is going on ten years of service:



--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29493368 -

Anyone get this error message when trying to upload pictures before?

"1000006475.jpg was bigger than the allowed file size of 5120 KB and was not saved."

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers] * 1
    #29493394 -

Yeah I got it yesterday and had to resize a photo.

And folks were complaining about it before that. Seems like a site issue of some kind.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29493405 -

I posted it Here. I'm having deja vu but I'm not looking for proof of otherwise. :lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #29493454 -

This thread moves super fast but a couple pages I posted my work around for IPhone.

Save the photo to files
Go to files and hold on pic for “quick actions”
Resize joeg and medium
Come here and “add picture” and use “files” and the resized will be selectable.
No issues yet


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29493459 -

the_chosen_one said:
Might have some clarity issues in LC but a side by side comparison to the standard PDA agar recipes is immediately where my brain went lol. Might have to try it on the next round.



It's probably not gonna be as crystal clear as a turbo refined culture medium but we'd definitely stay ahead of a lot of problems by clarifying it before everything has started to turn into a mush and the starches and pectins have started to link with and trap each other.

...on that note: Pectinase is a fairly cheap bulk ingredient in brewing and juice making. Add it right after pressing, let it do its thing, then let everything settle.
Possibly clarify further by adding amylase and freezing + filtering
Quote:
Thanks!
:manofapproval:


Any time. Glad to hear there's possibly something here.


--------------------
Time is nature's way of making sure that everything doesn't happen at once.

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Re: Ask Quick Questions, Get Quick Answers [Re: towndaze]
    #29493486 -

Does anyone here have any information about how long detection rates for psilocybin on a drug test anything more specific than just saying could be as little as 24hr and as much as 72 hr does anyone have more detailed answers
Regarding immunoassays or LC/Ms/Ms testing ?
I would like to see what information people could have here with psilocybin not being very stable of a chemical but what about being processed in your liver and tested in urine

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Re: Ask Quick Questions, Get Quick Answers [Re: Sealifefungi]
    #29493492 -

Sealifefungi said:
Laminar flow with the oven ?? I tried searching g could not find anything . I need to transfer some spores to an agar dish , everything is already poured and sealed . I have a STAB the honey got off amazon ( I know I know ) I haven’t set it up yet it’s huge . And don’t wanna set it up for one transfer . I can obtain laminar flow by running oven at 250 cracking door and placing a tray there ? True or false


I saw a video of PGT (philly golden teacher) doing that with pouring his agar plates the other day on youtube so im thinking that should work you can look it up and see what you think

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 2
    #29493495 -

Shortcuts do not pay off in this hobby, unless you like dealing with contaminated cultures and pathetic harvests.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29493498 -

Phaethon said:
Shortcuts do not pay off in this hobby, unless you like dealing with contaminated cultures and pathetic harvests.



I agree wouldn’t still air box also be considered as a shortcut tho technically

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Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33] * 3
    #29493501 -

Plates can be poured in open air.  It's not recommended, but hot liquid that's close to patuerization temperature is very different than germinating plates.

And there's a reason folks like PGT don't post here, because we would peer review all the bad advice and they'd lose subscribers.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Pickyourpoison33]
    #29493510 -

Can you describe the underlying physics that makes an open oven door work? Still air is pretty darn simple in comparison, and probably an order of magnitude more likely to prevent contamination.

A bunsen burner works by pulling air (and contaminants) toward it and up and away from your work, so working near the flame is relatively safe. But a still air box is still going to beat that for most people, and without having to burn fuel the whole time you're working.

The convection currents around an oven door are a lot more complex and variable (it's not just a constant flame, the temperature changes over short periods). Changing currents usually also implies turbulence, which will pull in spore containing dust from your environment and contaminate your work. It's just not worth the contamination risk for the small time saved (if any) over setting up a still air box.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 3
    #29493514 -

I have seen PGT post on Reddit if tells ya anything.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29493520 -

I would personally just use a still air box if I was doing agar just cheap and easy to make I was just telling OP that I have also seen people saying they use the oven

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 2
    #29493523 -

I've posted on Reddit :shrug:  For twenty days and then I got paranoid :lol:

Anyway Phatheon just explained why an oven door is not laminar flow.  And I explained why PGT could get away with pouring plates in front of one.

I've heard micro biology students learn to pour them in open air as part of their coursework.  Like I said, hot liquid is likely to kill most spores.

I don't know what the point of the oven was other than to give bad advice and generate content :shrug:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29493538 -

tree frog said:
I don't know what the point of the oven was other than to give bad advice and generate content :shrug:


Not saying this will work or anything because I haven’t ever touched agar yet you all probably know a lot better than me but I think the concept is being looked at as similar to a Bunsen burner is why people are spreading that information around


Just curious as to your opinion on detection timeframes for psilocybin on LC/MS//MS urine testing im curious because of how unstable psilocybin is due to oxidation

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Mono Tub Substrate   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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