tree frog said:

Second flush f0.

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fahtster said:

I swear I’ve posted this on the site but can’t for the life of me find it in the search function.  Maybe it was just discord and I only uploaded the pics here.. anyway, I came up with a way to “serial dilute” (spore stretching might be a better phrase) on the swab for doing grab and drags that I think you might find useful, TF.  Works great for both mono hunting and ms because you can pick where on the swab you want to pull the tuft from which will determine the quantity of spores with the gnd.  It’s a c/p from discord so I’ll quote it.

Quote:

put a drop of water on a swab tip and kind of “cut” into the very edge of a print to get a thin line of spores on the side of the swab..


Then I took another blank swab with a couple drops of water on it and pressed the two together where the spore line was vertically and rubbed up and down with the spored swab and while I did that I kept the blank swab still and spun the spored swab slowly (I don’t have pics of the process I only have two hands haha but I have after pics.)



You can see how there’s an even colored band of spores moving away from the original spore line that gets lighter and lighter until you can barely see anything in that last pic right before it reaches back to the spore line.  That’s where you’d take the tuft from.

The idea being that as the spores get more and more broken apart from the rubbing, they also get spread further and further apart.  So you have a higher chance of individual spores on the swab fibers. 

You could even not keep the blank swab in the same spot and turn it a quarter turn to a new blank spot half way through spinning the spored swab so you’re not transferring any new spores from the spot of the blank swab where it was being initially rubbed

You could also get a new second blank swab and stretch them even further by rubbing the new swab (and spinning it) against the spot of the original blank where the transferred spores were

Here’s a plate where I started with a thick line of spores and then used the tuft from just before I got back to the starting spore line.. so I couldn’t really see much of anything on the fibers



If you wanted to get more germination, you’d just pick an area closer to the starting point of the rub

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A little side note… using mono hunting can be great for getting single di cultures from the jump.  I didn’t scope them because I don’t have one but I let these two colonies intercept each other



and after I saw what looked like a shift in myc types growing off both (I assume they were both monos), I took a transfer and grew it out and that’s what gave me the red spore weza culture.

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Dj_karma_magnet said:

You fruiting these in a Martha tent?

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tultedspoon said:

I guess working towards making some agar hoping for more success.

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Should be a fun chapter.

hellofresh said:

tultedspoon said:

I guess working towards making some agar hoping for more success.

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Should be a fun chapter.

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I mean according to his last few accounts he tried agar with it will be a complete shit show

tultedspoon said:

edit: yeah it's dumb nothing is new.

I guess working towards making some agar hoping for more success.

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You wanna try doing a single successful cube BRF cake before you do anything, and watch a documentary. You have zero respect for the fungi kingdom.

I'd direct you to sources but it's been done 100 times and you just ignore it so - meh.

*It takes quite some doing to cause me to sour on a person - you've at least excelled in something here.

Generic said:

happy... ummm.... 18-year shroomiversary Kunta Kente.

for clarity, i'm talking about a full, wide mouth sized SFD under the lid with a hole, and a regular mason jar band being used.  The bands will eventually rust and catch, and need to be replaced/recycled, but with the amount of mason jars I use, I still have some A-grade bands saved for this purpose.  Lids will rust and occasionally get replaced when I want something fancier, but its usually not necessary.  All in all, no silicone gets used or wasted, but it does use more plastic/PTFE SFD material, so that's the trade off there.

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Generic said:

Lids will rust and occasionally get replaced when I want something fancier, but its usually not necessary.

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