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OfflineSaint
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Re: Liquid Culture De-Mystified: Ghia Style [Re: kotter]
    #19758886 - 03/28/14 06:07 AM (9 years, 9 months ago)

I know I will find  my answer to question the more I read. Still going to ask about Temp  to use  when making liquid culture or expanding  liquid culture in small 150cc jar's Is there a max  temp to use. My temp for these shiitake LC been from 86-100  degrees. seen to be ok only 2 days.

I  stir  by hand  as much as possible. the  drake specks  is from grain Turkey baster is to big. Using 3ml glass storage vials

I did have one issue
I made  some 1000 cc jars growth was great pulled most of the mycaleum  off used for sawdust  blocks so i know the LC was good. But I did make up sterile water and added 700cc to the jars. I all so used honey  to make them. Added 20cc of sterile honey. thinking it would help the mycelium to grow. I think i killed my jars.IT looks like brown crap/dust not fluffy like normal. Still learning the hard way. CAN SOMEONE ADD WATER/FOOD TO A LC JAR?


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Invisibleblindingleaf
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19758958 - 03/28/14 07:11 AM (9 years, 9 months ago)

86-100!?!?!  wow there captain sunbeam, lets bring it down! :lol:

shoot for no more than 80, i'd say less, like 78.  if u want faster growth, use less nutrients. (I'm using 1.5% sugars now, i think the norm is 4%)

the last part of ur post...not sure what u mean?  seems like u attempted either a GLC (or sawdust LC?) .  thats a good route (I never did it, do not trust it, but seems to work for ppl) but the nutrients are sorta kinda already in there, and come out when u suck the myc water back out (some nutrients are extracted from the short soak btw injection and sucking back up).  if u added honey to it, u would have needed to sterilize it.  even then, not the best idea.  what u should do, If I'm understanding ur goal correctly, is do a grain LC (shoot sterile water into sterile grain jar 100% colonized) then suck it back out, and shoot THAT into a pre made, pre sterilized LC.  u do not even have to go that far, that GLC is ready to use as it comes out of the grain jar, but if u wanna expand it, thats the route to take.

jake can chime in here, as he is way more experienced with edibles than i am, but i ONLY start LC's with an agar wedge.  i kno glc seems like a good idea, but i still don't trust it like i do with agar.  even an agar LC i will test before using.

also, cannot tell by the pics, but are u using an SAB or a hood?  that will increase ur success rate a thousand fold, with any part of this hobby.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineSaint
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #19759046 - 03/28/14 07:58 AM (9 years, 9 months ago)

shoot for no more than 80, i'd say less, like 78.  if u want faster growth, use less nutrients. (I'm using 1.5% sugars now, i think the norm is 4%)

Thank's for the temp advise I  was just playing around to see what temp change would do. normally  i do have them sitting in  cobert around 80 degrees. Just this time  was trying something new  with  the LC And had to ask what effect temp does have. other them contaminants grow better  at higher temps.

2) the last part of ur post...not sure what u mean?  seems like u attempted either a GLC (or sawdust LC?) .

No, not a  glc or sawdust LC. I Have  akot of them bags already
IT's a straight LC I ll  take a photo of what happen when i added water and honey {yes all sterilize}.  added a photo in minute be right back


I have to remove some photos it looks like  before  i can upload new ones as i could not upload. thanks for  let me know about the 1.5 % i ll try  that


Edited by Saint (03/30/14 03:18 PM)


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Offlineinvitro


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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19759606 - 03/28/14 11:49 AM (9 years, 9 months ago)

What variety of shitake do you have?  I think there are cold weather and warm weather types.


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OfflineSaint
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Re: Liquid Culture De-Mystified: Ghia Style [Re: invitro]
    #19769146 - 03/30/14 03:14 PM (9 years, 9 months ago)

I am not even sure which one i have growing  right now i have  like 5 different ones I growing now waiting to see the fruit to see which is going to be a few months  yet. KMH,DCL,96,SBW

Right now trying to figure out how to do this spore  growth with Petri dish. And its just pissing me off  with these cubes  first black mold/green mold now I am brown  contam's When i get one good dish i will be happy. The shiitake  has be  very easy to keep clean nothing  get contam so  I know its the spore syringe's

Forgot to say i started this on 01/01/2014 I never did this before. But i do have almost 100 blocks made up all ready. IT's been very easy  you just need right equipment.


Edited by Saint (03/30/14 03:26 PM)


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Invisibleblindingleaf
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19769201 - 03/30/14 03:25 PM (9 years, 9 months ago)

that sucks man, i had an unlucky cube print like that once.  do u have any current grows u can clone from?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineSaint
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #19769229 - 03/30/14 03:31 PM (9 years, 9 months ago)

Quote:

blindingleaf said:
that sucks man, i had an unlucky cube print like that once.  do u have any current grows u can clone from?



Tell me about it. at least I learning  how to do everything first hand. I got ahold of vendor on here and  he  replaced my first synge with 3 new ones. So  he was very good to me.  i am sure I will get one to work. Just the time is really the only thing  I am out. No cubes as of  yet But i am learning about spores.


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Invisibleblindingleaf
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19769240 - 03/30/14 03:33 PM (9 years, 9 months ago)

nice!  I'm sure at least one of those will be clean.  i have only had one bad syringe.  u can also hit the marketplace and do trades.  maybe if u have an edible culture u can trade that for a cube print or two.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineSaint
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #19769277 - 03/30/14 03:41 PM (9 years, 9 months ago)

Quote:

blindingleaf said:
nice!  I'm sure at least one of those will be clean.  i have only had one bad syringe.  u can also hit the marketplace and do trades.  maybe if u have an edible culture u can trade that for a cube print or two.





Good idea I am just not ready  yet I have only bout 70 master 3 ml vials made up so far. trying to keep my self supplied with grain spawn is my main  idea right now. i want to  make at least 100 blocks per month so i can sell them at framers market with the mushrooms I grow. Its my goal to  have a part time job from a hobby.


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Invisibleblindingleaf
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19769292 - 03/30/14 03:44 PM (9 years, 9 months ago)

100 blocks!! jeez.  i live in a place thats too hot for cold species in the summer like shiitaki.  i'd need to run the AC window unit a lot, even then, it still is about 80 minimum in my 3rd floor apt.  i have 2 shiitaki bags going now, pop corning.  I'm hoping they beat the warm weather that is surely coming.  just got our first big spring rain all day yesterday, continuing on right now.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinecronicrFacebook
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #19769316 - 03/30/14 03:47 PM (9 years, 9 months ago)

yeah i kinda missed my shitake window:lol: lazy stoner season


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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #19769396 - 03/30/14 04:00 PM (9 years, 9 months ago)

Well I don't worry about elc its in my rent for  a apt on the 16th floor. I have  the green house set up in front of a 12 foot window with a AC unit right on the same wall. Have a walk in closet i am using to store the blocks in the dark ruff 78 degrees so i am  just waiting to start see them all browning at one time. I am not worried about cost and will sell blocks for $15 at the market just enough to cover  my cost. With some extra But if I sell a bag of grown your going to pay for them! Retired in 2004 so its just a hobby to me.


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Invisibleblindingleaf
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
    #19769599 - 03/30/14 04:43 PM (9 years, 9 months ago)

oh u mean ur selling kits?  i thought u meant actual fruits


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinemadLyfe
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
    #20122494 - 06/13/14 12:38 AM (9 years, 7 months ago)

i am using the TL's not tilt tek, but was wondering what other things could be used to break up the LC other than rocks?


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Offlineghiajake
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Re: Liquid Culture De-Mystified: Ghia Style [Re: madLyfe]
    #20655885 - 10/04/14 12:47 AM (9 years, 3 months ago)

Quote:

madLyfe said:
i am using the TL's not tilt tek, but was wondering what other things could be used to break up the LC other than rocks?




Best thing is a magnetic stirrer.


Just noc'd up a new PM LC a couple days ago. This time I used grass clipping/reishi tea (boiled hard for an hour) as the nutrient base. Been using this as my agar nutrient base with good success so I figured I'd try it in LC too. I'll post a pic in a couple days.


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Invisibleeatyualive
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Re: Liquid Culture De-Mystified: Ghia Style [Re: ghiajake]
    #20662205 - 10/05/14 03:59 PM (9 years, 3 months ago)

beautiful work!

makes me want to bust out my ancient stir plate and stir bar.


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EAT GETS SHIT DONE


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Edited by eatyualive (10/05/14 04:00 PM)


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