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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: kotter]
#19758886 - 03/28/14 06:07 AM (9 years, 9 months ago) |
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I know I will find my answer to question the more I read. Still going to ask about Temp to use when making liquid culture or expanding liquid culture in small 150cc jar's Is there a max temp to use. My temp for these shiitake LC been from 86-100 degrees. seen to be ok only 2 days.
    I stir by hand as much as possible. the drake specks is from grain Turkey baster is to big. Using 3ml glass storage vials 
I did have one issue I made some 1000 cc jars growth was great pulled most of the mycaleum off used for sawdust blocks so i know the LC was good. But I did make up sterile water and added 700cc to the jars. I all so used honey to make them. Added 20cc of sterile honey. thinking it would help the mycelium to grow. I think i killed my jars.IT looks like brown crap/dust not fluffy like normal. Still learning the hard way. CAN SOMEONE ADD WATER/FOOD TO A LC JAR?
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19758958 - 03/28/14 07:11 AM (9 years, 9 months ago) |
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86-100!?!?! wow there captain sunbeam, lets bring it down! 
shoot for no more than 80, i'd say less, like 78. if u want faster growth, use less nutrients. (I'm using 1.5% sugars now, i think the norm is 4%)
the last part of ur post...not sure what u mean? seems like u attempted either a GLC (or sawdust LC?) . thats a good route (I never did it, do not trust it, but seems to work for ppl) but the nutrients are sorta kinda already in there, and come out when u suck the myc water back out (some nutrients are extracted from the short soak btw injection and sucking back up). if u added honey to it, u would have needed to sterilize it. even then, not the best idea. what u should do, If I'm understanding ur goal correctly, is do a grain LC (shoot sterile water into sterile grain jar 100% colonized) then suck it back out, and shoot THAT into a pre made, pre sterilized LC. u do not even have to go that far, that GLC is ready to use as it comes out of the grain jar, but if u wanna expand it, thats the route to take.
jake can chime in here, as he is way more experienced with edibles than i am, but i ONLY start LC's with an agar wedge. i kno glc seems like a good idea, but i still don't trust it like i do with agar. even an agar LC i will test before using.
also, cannot tell by the pics, but are u using an SAB or a hood? that will increase ur success rate a thousand fold, with any part of this hobby.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#19759046 - 03/28/14 07:58 AM (9 years, 9 months ago) |
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shoot for no more than 80, i'd say less, like 78. if u want faster growth, use less nutrients. (I'm using 1.5% sugars now, i think the norm is 4%)
Thank's for the temp advise I was just playing around to see what temp change would do. normally i do have them sitting in cobert around 80 degrees. Just this time was trying something new with the LC And had to ask what effect temp does have. other them contaminants grow better at higher temps.
2) the last part of ur post...not sure what u mean? seems like u attempted either a GLC (or sawdust LC?) .
No, not a glc or sawdust LC. I Have akot of them bags already IT's a straight LC I ll take a photo of what happen when i added water and honey {yes all sterilize}. added a photo in minute be right back
I have to remove some photos it looks like before i can upload new ones as i could not upload. thanks for let me know about the 1.5 % i ll try that
Edited by Saint (03/30/14 03:18 PM)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 19 days
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19759606 - 03/28/14 11:49 AM (9 years, 9 months ago) |
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What variety of shitake do you have? I think there are cold weather and warm weather types.
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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: invitro]
#19769146 - 03/30/14 03:14 PM (9 years, 9 months ago) |
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I am not even sure which one i have growing right now i have like 5 different ones I growing now waiting to see the fruit to see which is going to be a few months yet. KMH,DCL,96,SBW
Right now trying to figure out how to do this spore growth with Petri dish. And its just pissing me off with these cubes first black mold/green mold now I am brown contam's When i get one good dish i will be happy. The shiitake has be very easy to keep clean nothing get contam so I know its the spore syringe's
Forgot to say i started this on 01/01/2014 I never did this before. But i do have almost 100 blocks made up all ready. IT's been very easy you just need right equipment.
Edited by Saint (03/30/14 03:26 PM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19769201 - 03/30/14 03:25 PM (9 years, 9 months ago) |
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that sucks man, i had an unlucky cube print like that once. do u have any current grows u can clone from?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#19769229 - 03/30/14 03:31 PM (9 years, 9 months ago) |
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Quote:
blindingleaf said: that sucks man, i had an unlucky cube print like that once. do u have any current grows u can clone from?
Tell me about it. at least I learning how to do everything first hand. I got ahold of vendor on here and he replaced my first synge with 3 new ones. So he was very good to me. i am sure I will get one to work. Just the time is really the only thing I am out. No cubes as of yet But i am learning about spores.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19769240 - 03/30/14 03:33 PM (9 years, 9 months ago) |
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nice! I'm sure at least one of those will be clean. i have only had one bad syringe. u can also hit the marketplace and do trades. maybe if u have an edible culture u can trade that for a cube print or two.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#19769277 - 03/30/14 03:41 PM (9 years, 9 months ago) |
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Quote:
blindingleaf said: nice! I'm sure at least one of those will be clean. i have only had one bad syringe. u can also hit the marketplace and do trades. maybe if u have an edible culture u can trade that for a cube print or two.
Good idea I am just not ready yet I have only bout 70 master 3 ml vials made up so far. trying to keep my self supplied with grain spawn is my main idea right now. i want to make at least 100 blocks per month so i can sell them at framers market with the mushrooms I grow. Its my goal to have a part time job from a hobby.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19769292 - 03/30/14 03:44 PM (9 years, 9 months ago) |
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100 blocks!! jeez. i live in a place thats too hot for cold species in the summer like shiitaki. i'd need to run the AC window unit a lot, even then, it still is about 80 minimum in my 3rd floor apt. i have 2 shiitaki bags going now, pop corning. I'm hoping they beat the warm weather that is surely coming. just got our first big spring rain all day yesterday, continuing on right now.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#19769316 - 03/30/14 03:47 PM (9 years, 9 months ago) |
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yeah i kinda missed my shitake window lazy stoner season
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Saint
Pope



Registered: 12/05/13
Posts: 76
Last seen: 4 years, 5 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#19769396 - 03/30/14 04:00 PM (9 years, 9 months ago) |
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Well I don't worry about elc its in my rent for a apt on the 16th floor. I have the green house set up in front of a 12 foot window with a AC unit right on the same wall. Have a walk in closet i am using to store the blocks in the dark ruff 78 degrees so i am just waiting to start see them all browning at one time. I am not worried about cost and will sell blocks for $15 at the market just enough to cover my cost. With some extra But if I sell a bag of grown your going to pay for them! Retired in 2004 so its just a hobby to me.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
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Re: Liquid Culture De-Mystified: Ghia Style [Re: Saint]
#19769599 - 03/30/14 04:43 PM (9 years, 9 months ago) |
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oh u mean ur selling kits? i thought u meant actual fruits
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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madLyfe
Wonderer



Registered: 04/05/14
Posts: 27
Last seen: 1 year, 9 months
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Re: Liquid Culture De-Mystified: Ghia Style [Re: blindingleaf]
#20122494 - 06/13/14 12:38 AM (9 years, 7 months ago) |
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i am using the TL's not tilt tek, but was wondering what other things could be used to break up the LC other than rocks?
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ghiajake
Myco-Viking


Registered: 01/10/13
Posts: 3,846
Loc: Indiana
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Re: Liquid Culture De-Mystified: Ghia Style [Re: madLyfe]
#20655885 - 10/04/14 12:47 AM (9 years, 3 months ago) |
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Quote:
madLyfe said: i am using the TL's not tilt tek, but was wondering what other things could be used to break up the LC other than rocks?
Best thing is a magnetic stirrer.
Just noc'd up a new PM LC a couple days ago. This time I used grass clipping/reishi tea (boiled hard for an hour) as the nutrient base. Been using this as my agar nutrient base with good success so I figured I'd try it in LC too. I'll post a pic in a couple days.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid Culture De-Mystified: Ghia Style [Re: ghiajake]
#20662205 - 10/05/14 03:59 PM (9 years, 3 months ago) |
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beautiful work!
makes me want to bust out my ancient stir plate and stir bar.
Edited by eatyualive (10/05/14 04:00 PM)
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