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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29476495 -

Huskies said:
I think so, put it on a new plate and see if it grows well



Its a good sign if the right is in the lines where I streaked the swab right?

Forgive the ignorance. Spore swabs are a new territory for me with Agar.


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Successful Grows: ONE

Agar Plates Gone Through: 46
Agar Plates successfully cultivated: 6

Edited by ladynightshade (02/07/26 12:53 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476498 -

Yes, that is exactly what you want.

Don't throw out the plate after getting the transfer, see if you get clean growth throughout your swab path


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29476502 -

Yay! That means I have six plates that managed to cultivate. Just going to eye them for now and transfer when there's more covered.


--------------------
Successful Grows: ONE

Agar Plates Gone Through: 46
Agar Plates successfully cultivated: 6

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476516 -



Got a couple okay looking pins but most of them have white ridges and dots, I’m guessing it’s vert but not sure, variety is blue meanies. Input?:shrug:

Edit: Dark spot is from a lid mishap just damaged sub


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Edited by toadking (02/07/26 01:23 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29476558 -

BeefExtreme said:
levitation chamber?


I would read that tek :yesnod:


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc]
    #29476591 -

So I have some previously pasteurized straw in a cooler, where it was pasteurized, months ago.

It never molded at all.

Is that safe to use, or should it just be thrown out or re-pasteurized?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29476616 -

Should be fine.. though I'd mix it up real good before use. Gravity has likely pulled the moisture to the bottom.

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476638 -

ladynightshade said:
Spore swabs sort of escape me and Im never too sure if I hit contam or not.





Its easier to tell with spore prints, but did these cultivate any mycelium?


There is a good chance you have myc there. I agree, tho. Sometimes they look suspicious as gosh darn heck. Looks like your parafilm slipped on one or more of your plates. Try stretching it a little more to get a better grip/seal. You're going to want that plate sealed if you are like me and cant keep your hands off of 'em! :lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29476689 -

^ What he said.

I find that stretching the the parafilm BEFORE wrapping the plate helps a lot. Pre-stretching makes it so the film doesn't snap off easily.

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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29476729 -

Gilmour Hendrix said:
ladynightshade said:
Spore swabs sort of escape me and Im never too sure if I hit contam or not.





Its easier to tell with spore prints, but did these cultivate any mycelium?


There is a good chance you have myc there. I agree, tho. Sometimes they look suspicious as gosh darn heck. Looks like your parafilm slipped on one or more of your plates. Try stretching it a little more to get a better grip/seal. You're going to want that plate sealed if you are like me and cant keep your hands off of 'em! :lol:



Will do. I like double checking them from time to time, and as simple as parafilm is, its such a learning curve to start.


--------------------
Successful Grows: ONE

Agar Plates Gone Through: 46
Agar Plates successfully cultivated: 6

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476833 -

if i wanna get more into isolating genetics is it worth it to learn brf/pf tek?


--------------------
buy the ticket , take the ride

here and here are good places to start

:bunnypeace:

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Re: Ask Quick Questions, Get Quick Answers [Re: squigglez] * 2
    #29476837 -

Cakes are a very good skill to have with a lot of applications, but for culture work you want to practice your sterile technique ie agar, lc, and whatnot...


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476946 -

ladynightshade said:
Gilmour Hendrix said:
ladynightshade said:
Spore swabs sort of escape me and Im never too sure if I hit contam or not.





Its easier to tell with spore prints, but did these cultivate any mycelium?


There is a good chance you have myc there. I agree, tho. Sometimes they look suspicious as gosh darn heck. Looks like your parafilm slipped on one or more of your plates. Try stretching it a little more to get a better grip/seal. You're going to want that plate sealed if you are like me and cant keep your hands off of 'em! :lol:



Will do. I like double checking them from time to time, and as simple as parafilm is, its such a learning curve to start.


Just don’t get too upset or discouraged when you eventually go to wrap the parafilm around the plate and a transfer slides all around.  lol.


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29476962 -

Seeking advise for pressure cooking 15 psi, 4oz BRF PF-tek Jars. I want to ensure the jars are sterilized without dehydration. 4oz jars, brown rice, vermiculite, water. How long? Standard advise is for 8oz jars, which may be too long and thus dehydrate the 4oz jars.

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Re: Ask Quick Questions, Get Quick Answers [Re: HydroShroom24] * 2
    #29476998 -

Rev recommends 90 minutes for that size in the PC: https://www.shroomery.org/forums/showflat.php/Number/28649340#28649340 (I'm pretty sure that's what I did last time I made small BRF pucks too).

Dehydration isn't a big concern since we're sterilizing with penetrating steam. More important to make sure the cycle is long enough to actually sterilize the substrate. (That said, 90 minutes, while it won't hurt it, is probably overkill for 4 oz jars. I think even grain in that small of a jar can get by with 60 minutes.)


--------------------
LAGM 2026 grow log | My library/trade list

Edited by Phaethon (02/07/26 09:19 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29477166 -

I have an old BRF puck from 11/24 that is dried up and shrunk away from the walls - is it worthwhile to try to transfer to agar or is there a better way?  The puck looks like its covered in nice clean myc still.

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Re: Ask Quick Questions, Get Quick Answers [Re: MetalPro]
    #29477176 -

sure, that’s fine to do. If i were to do it, I would, inside a SAB or in front of a flow hood: by hand, tear the cake in half, top to bottom (or however it works out) and use a sterilized scalpel to take a small sample of mycelium from the freshly exposed interior of the cake and place that on agar.

Maybe do 2 plates that way. You’ll likely get a clean culture on agar that way. In a few days, if you start to see contamination on the plate (like wet goo), no worries, just take another transfer to a fresh plate and grow it out.

Good luck!


--------------------
Peace to you, friend!
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GROWER’S BEST photo contest: join the fun!
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Re: Ask Quick Questions, Get Quick Answers [Re: flapping_bird]
    #29477275 -

Hey Shroomers,

How long would a quart of rye last in the fridge after its been sterilised?

Thank you kindly :grin:


--------------------
All the world’s a stage,
And all the men and women merely players;
They have their exits and their entrances.

Edited by Libertycap97 (02/08/26 04:09 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29477341 -



Just don’t get too upset or discouraged when you eventually go to wrap the parafilm around the plate and a transfer slides all around.  lol.


I've had good luck with cutting into the receiving agar first and when I cut the transfer I push it down into that hole. It sticks right there, could be lack of many attempts but I've got good luck doing that!


--------------------
The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: Libertycap97] * 4
    #29477347 -

Libertycap97 said:
Hey Shroomers,

How long would a quart of rye last in the fridge after its been sterilised?

Thank you kindly :grin:


When stored in a modified jar with a filter or an unmodified jar with a secure lid, the contents can last for months, provided they are properly sterilized. Additionally, refrigeration is not necessary for long-term storage if you prefer not to use it. Personally, I keep oats on a shelf for months before using them. Just ensure that they are kept in a jar with a reliable lid, and you should be all set.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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