zimmey said:

Mackload said:

zimmey said:

g2g from seemingly clean spawn

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This might be your problem right here.

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The agar looked clean that it came from. I know it is possible to have hidden bacteria in the plate... and I hadn't gone to water agar or anything, but there was definitely no obvious problems with the agar that made the master. It colonized very quickly and consistently. It is definitely a possible vector.

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ModularMind said:

What grain?

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Phaethon said:

I find it hard to tell whether grain is fully free of bacteria, much easier with agar or low nutrient (0.2%) LC.

Can you say more about your flow hood? There are a lot of cheap DIY "flow hood" designs out there now which are terrible (not designed for actual laminar flow, assuming they even filter well enough in the first place).

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cronicr said:

zimmey said:

Mackload said:

zimmey said:

g2g from seemingly clean spawn

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This might be your problem right here.

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The agar looked clean that it came from. I know it is possible to have hidden bacteria in the plate... and I hadn't gone to water agar or anything, but there was definitely no obvious problems with the agar that made the master. It colonized very quickly and consistently. It is definitely a possible vector.

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Skip the g2g , it's easier to make more agar dishes then it is to g2g

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Mackload said:

Maybe pressure cook longer? Are you all gloved up and iso'd? Clean tools for transfers? Got pics of your agar? Proper flow hood? I'd guess it's your spawn or technique more than tinfoil or lid type. Imho

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zimmey said:

The last several grain spawns attempts I've tried have resulted in sub-optimal results (~50% bacterial contamination).

I think I've done three different batches recently. I wanted to use no-modified lids, and I was trying yoshi's no rinse/no soak tek. I wasn't using tin foil. I also used the simmer for a few minutes then dry in a colunder method for the last attempt.

I pressure cooked at 17psi+ for 90 mins, and last one 120 mins and I opened the pressure cooker in front of a flow hood, and was doing g2g from seemingly clean spawn (as some of the jars have 0 bacterial using the same master grains).

It seems that yoshi's method should work, and I'm interested in less manual work to get spawn working... so my next attempt is to use foil to help protect the tops of the jars, and to use micropore tape instead of no-mod lids.

Any input on this on how to make the grain spawn more bullet proof? It is really disheartening to get to the end and have the jars go sour.

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RhyzoPhreak said:

I did 24 jars with this method and I am very happy with the results. I could have dried it a little longer but it still worked out. Next time I won't do as much at once, it was a pain in the ass.

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Peleus said:

Inocculated 5 days ago, how do they look?


Each had about half a petri of thicc wedges.

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flapping_bird said:

it is very easy for the amount of dried fruits to overwhelm my own rate of use, so I do my best to try to keep it pared down to just a few trips worth.  Even so, it breaks my heart and feels wasteful to destroy/toss perfectly fine shrooms.

With it being so easy to grow a fresh batch when needed, why hold onto a metric fuckton? I do get it though…

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Splittinghairs said:

flapping_bird said:

it is very easy for the amount of dried fruits to overwhelm my own rate of use, so I do my best to try to keep it pared down to just a few trips worth.  Even so, it breaks my heart and feels wasteful to destroy/toss perfectly fine shrooms.

With it being so easy to grow a fresh batch when needed, why hold onto a metric fuckton? I do get it though…

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My friends love me is all I’m going to say.

I find the cult part more fascinating than anything

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