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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29466890 -

Nokumana said:
shroomerhumor said:
The two bottom jars look good to me. Very nice growth, I'm jealous. I don't know if my eye is playing tricks on me but the grains in the 2nd jar from the 1st pic look drier compared to the 2nd jar from the second pic. That's just my opinion though.

Did you cut a grain or two throughout cooking to make sure the interior was fully hydrated? Are all of those jars using grain from one cook batch? How tight are those unmodified lids?


All jars from the same batch.

I tightened the unmodified lids and then back them off about a quarter to a third of a turn.

The top two jars were exhibiting growth that was very similar to if not even more extensive than the bottom two jars. I shook them up and now that growth is sort of evenly distributed throughout. You can see a piece of agar in the top left photo as well, but I don't see anything that looks like frank contamination to me.

Anything that I can do at this point to help them along, or just LITFA?


Just for clarity, and it’s only cause I was just literally going through this entire fiasco……..

When you say a quarter of a turn,

Do you mean a quarter of the lid diameter or a quarter of an inch back off.

It makes a huge difference. Quarter turn of the lid diameter is too much apparently. I would put agar to jars, crap is kicking ass, then slow putter stall to completion.

When I read through Pasty’s lid Tek in my sig to see where I was screwing it up, he clarify’s it needs to be quarter of an inch.


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Favorite Teks

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Re: Ask Quick Questions, Get Quick Answers [Re: ihansterx4i] * 2
    #29466970 -

ihansterx4i said:
Is there any other way to test if this flowhood is usable or not? I did the flame test and it seemed good. Other than buying was one of those particle readers, is there no other way?


The real way to test it is with plates, but you can't just open them and leave them in place. You need to move your hands and tools around them the way you would when doing clean work. Just skip the actual transfers. Then wait and see what grows.

The reason that just opening plates is not good enough is that these do move filtered air. That is great. But what they do not do is provide laminar flow. The problem with that is when you insert anything into the turbulent flow near the sterile surfaces you draw in a mix of all of the dirty air around the narrow band of clean filtered air. That causes a mix of clean and dirty air to deposit all kinds of undesirable schmutz onto your clean surfaces.

A proper laminar flow that is large enough gives you an area where you can move around that doesn't mix with the unclean air around the work surfaces. It is not just about a band of clean air. It is about a large area of clean air that is moving is such a a way that it doesn't mix with the unclean air around that clean tube of air.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ihansterx4i]
    #29466974 -

ihansterx4i said:
Is there any other way to test if this flowhood is usable or not? I did the flame test and it seemed good. Other than buying was one of those particle readers, is there no other way?


Nope, it’s trash.


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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29466978 -

So am i getting this right that your assessment is that the front of the filter is not suitable for laminar flow and will likely pull contaminates into the work area. Even if its a "hepa" filter connected to a suitable fan.

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Re: Ask Quick Questions, Get Quick Answers [Re: wobblyboxes]
    #29466980 -

Yes. Hepa insures that the air is clean, but not that it is laminar. Great for reducing the particulate in a volume or air over time with multiple cycles. Useless for creating a clean work space in front of the filter.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29466990 -

so while we're at it...   

how to FFU's work, then?  They're not laminar, right?

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Re: Ask Quick Questions, Get Quick Answers [Re: SuperNoz]
    #29466997 -

Yeah not ideal to get side fruits but they're not ALL on the sides. Don't beat yourself up it looks to me like you're doing a good job of conditions. Can always water but drain off excess after a day don't let it pool.

Let em flush and then do beef's punish tek.


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Official Ps papuana Cultivation Thread
exotic indexes: Ps zapotecorum varieties, Ps papuana
my grow logs: Ps neoxalapensis, Ps baeocystis, Ps papuana

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Re: Ask Quick Questions, Get Quick Answers [Re: Generic] * 1
    #29467000 -

Yes. Look at the filters on an FFU. Those things are several inches thick, made with a specific baffle pattern and cost a couple hundred dollars for a 24x24 inch and more for larger. That is a big difference to the furnace hepa filters. They still take a bit of dialing in to get the correct back pressure for operation in the laminar range.

They were not originally designed for laminar flow. But they do use filters capable of laminar flow with the correct baffles and back pressure plenum and static pressure. In theory the ones sold specifically for mushrooms say that they are laminar and have been dialed in correctly. But I would still do a smoke test and play with the fan speed and prefilter to make sure that you are staying in the right range.

Edit: to correct a couple less accurate terms.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (01/28/26 05:56 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 1
    #29467006 -

ok great, thanks rev.  i'm finally understanding more...

but flow hoods have very thick filters too, and a blower, too.  I guess the only difference is the plenum?

It was very unclear to me the minimum size a plenum needs to be to construct a laminar flow hood.  so i designed it with a big plenum...  now i regret that because of how much space it takes up and how heavy the whole thing is.

do flow hoods and ffu's use the same type of filters?

if you changed the dimensions of a laminar flow hood's plenum to be smaller and smaller, would be approaching, in effect, a FFU?

And FFU's are popular because they're smaller and cheaper, but don't work as ideally?

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Re: Ask Quick Questions, Get Quick Answers [Re: Generic] * 2
    #29467017 -

FFUs are cheaper, smaller and have a more limited safe work zone, but they are good enough for cult work. The filters are the same or close. There are better filters for more stringent requirements of some proper flow hood applications. Flow hoods generally also draw air at the back end of the clean zone as well to increase the laminar clean zone.

The plenum is there to prevent variability in the pressure of the air striking the back of the filter. Ideally you want the same static pressure of air across the entire surface of the back of the filter in both. A good flow hood design is more stringent with that requirement. The FFUs do the minimum necessary to get away with it and play a little more fast and loose with that requirement. Again, good enough for what we do. I wouldn't trust one for anything medical, dangerous or where anyone's health and safety is on the line.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 2
    #29467044 -

What happens to all the white in a jar when I break it up? Like, a fully colonized jar will lose almost all its whiteness just because I broke the grains apart


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The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: hidingbush] * 3
    #29467082 -

hidingbush said:
What happens to all the white in a jar when I break it up? Like, a fully colonized jar will lose almost all its whiteness just because I broke the grains apart


It's all still there.  It's just been pulled apart and squished up.  It'll all recover in a few days and your jar will be white again.


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: hidingbush] * 2
    #29467132 -

hidingbush said:
What happens to all the white in a jar when I break it up? Like, a fully colonized jar will lose almost all its whiteness just because I broke the grains apart



Thats the magic in magic mushrooms!
Seriously though when you shake the jar, you are physically tearing the interconnected web of mycelium into thousands of tiny individual pieces. Each grain becomes a "node" of fungal life. Instead of one large organism, you now have hundreds of small colonies.

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc] * 2
    #29467211 -

andymc said:
hidingbush said:
What happens to all the white in a jar when I break it up? Like, a fully colonized jar will lose almost all its whiteness just because I broke the grains apart


It's all still there.  It's just been pulled apart and squished up.  It'll all recover in a few days and your jar will be white again.



It's just crazy that it can appear so thick and dominant and then be gone


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The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: hidingbush] * 11
    #29467325 -

hidingbush said:
It's just crazy that it can appear so thick and dominant and then be gone


That's what she said.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29467441 -



Hello my old friend…

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Re: Ask Quick Questions, Get Quick Answers [Re: ihansterx4i]
    #29467443 -

Now just seal your bags after the PC cycle and inject with lc , far less fumbling around.


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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 5
    #29467448 -


I rocked this set up for a couple years it worked great , bags in the bottom still air box on top



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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29467454 -

Can I seal bags before pressure cooking if im planning on injecting it with liquid culture?


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Re: Ask Quick Questions, Get Quick Answers [Re: RhyzoPhreak]
    #29467484 -

They will come out of the PC sealed enough , you can seal them after the PC cycle once cooled then hang them to draw air through the filter


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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