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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29453995 -

Hey dude! Hope all is well!

Thanks for the link. I’ll definitely check it out.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29453999 -

Nokumana said:
Makes a lot of sense. But just to be clear, the idea is to streak the plate in one way or another so that you can make sure to pick up a really clean colony to subsequently germinate?


I’m pretty sure the idea is to just ensure the LC is clean. Should be germinated already. Unless you are talking about a spore syringe.

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend] * 1
    #29454010 -

AstroSporeFiend said:
Nokumana said:
Makes a lot of sense. But just to be clear, the idea is to streak the plate in one way or another so that you can make sure to pick up a really clean colony to subsequently germinate?


I’m pretty sure the idea is to just ensure the LC is clean. Should be germinated already. Unless you are talking about a spore syringe.


You're right, ASF. I was unclear about what I was asking, and was careless about terminology. Yes, the LC is germinated already.

You and Kloakk answered my question...Plate/streak it to test for contamination.

What, if anything, do you then do with that "test" plate if it looks clean? Toss?  Transfer and propagate to have more of that culture on agar?  I guess the options are manifold.

Maybe what I'm asking is, what do most folks do with it?

And also, I believe I'm overthinking this...Shutting up now
:stanhopefacepalm:


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29454017 -

Nokumana said:
AstroSporeFiend said:
Nokumana said:
Makes a lot of sense. But just to be clear, the idea is to streak the plate in one way or another so that you can make sure to pick up a really clean colony to subsequently germinate?


I’m pretty sure the idea is to just ensure the LC is clean. Should be germinated already. Unless you are talking about a spore syringe.


You're right, ASF. I was unclear about what I was asking, and was careless about terminology. Yes, the LC is germinated already.

You and Kloakk answered my question...Plate/streak it to test for contamination.

What, if anything, do you then do with that "test" plate if it looks clean? Toss?  Transfer and propagate to have more of that culture on agar?  I guess the options are manifold.

Maybe what I'm asking is, what do most folks do with it?

And also, I believe I'm overthinking this...Shutting up now
:stanhopefacepalm:


Haha! Chill, dude. Do whatever you want. I’d probably wait for some growth, transfer a nice piece to a new plate, and keep the culture. That way you have a backup if you use your LC and you get a moldy jar or something else goes wrong.

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend]
    #29454034 -

Ha.  Yeah, I was spiraling into a vortex of questions and endless permutations.  It's been an intense few weeks on the learning curve.

Getting out of the weeds and back to gazing at the forest.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29454038 -

Trust me, as a noob myself, I totally understand. I just posted a pic on the Agar thread for help because I have no idea what I’m looking at. Lol.

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend]
    #29454040 -

I saw that...to my untrained eye I thought it looked pretty good. :smile:


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29454041 -

Meh, who knows? :shrug:

All of us noobs are learning and it’s fun.

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Re: Ask Quick Questions, Get Quick Answers [Re: shroomerhumor]
    #29454085 -

shroomerhumor said:
My LC syringe from vendor also had little black spots. They explained that it was from the rust of the metal lid where the ports were drilled.. so far no problem on my side.


That seems like a very implausible explanation. Rust is red(dish) and heavy. It will never float around. Black rust forms under near anaerobic circumstances. But the holes drilled would have been for recovery of LC and gas exchange (can't take out fluid without something replacing it in the jar). So the gas exchange opening, where you'd expect the highest O2 levels, would display near-anaerobic phenomena?

Not saying it's impossible, or there's something wrong with your LC, it just doesn't make much sense to me.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana] * 1
    #29454086 -

If a plate is clean, it's clean. You can use it, even if you streaked and nothing germinated.
I do, in fact, have bispo T1s on plates that have been around for months, and have contained dried grains for two months. Even opened the plates after about a month to spuirt in some sterile water.
The grains didn't bounce back and now there's bispo growing on them. Waste not want not


--------------------
No nation, no border
Fight law and order


Species grown so far

Edited by Churpee Choozle (01/13/26 05:26 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29454155 -

To me this jar looks to be bacterial, just shook it and a lot of liquid appeared as well. Was some uncolonized wet grains up against the side before. This is a jar of semperviva if that matters, no off smell coming through the airhole though, and I think there might be some condensation forming from a cold front hitting here. Any thoughts on the jar?


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Re: Ask Quick Questions, Get Quick Answers [Re: DiscoDave]
    #29454163 -

I decided to transfer the agar because of the little spots but after I opened it I think its mycelium I did the transfer in SAB then looked at it and took the photo in non sterile conditions so now im tossing it and growing the agar out so its circular growth it helps me detect contamination. The agar has dirt from the floor added to it even contains hair because I don't want it to stick in the jar it helps. Its been sterilized though so its not really a contamination just looks kinda funny :shrug:

Is it contamination free?


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: DiscoDave]
    #29454169 -

DiscoDave said:
To me this jar looks to be bacterial, just shook it and a lot of liquid appeared as well. Was some uncolonized wet grains up against the side before. This is a jar of semperviva if that matters, no off smell coming through the airhole though, and I think there might be some condensation forming from a cold front hitting here. Any thoughts on the jar?




What did you inoculate it with? Agar or LC

How long ago was it pressure cooked? How long since inoculation?


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: DiscoDave]
    #29454170 -

DiscoDave said:
To me this jar looks to be bacterial, just shook it and a lot of liquid appeared as well. Was some uncolonized wet grains up against the side before. This is a jar of semperviva if that matters, no off smell coming through the airhole though, and I think there might be some condensation forming from a cold front hitting here. Any thoughts on the jar?




Curious to what kind of jar you are running your grain in?

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29454174 -

You'll know when it grows out


--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: DiscoDave] * 2
    #29454175 -

Definitely contaminated.  And same question as above about the jar...


--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29454176 -

Splittinghairs said:
What did you inoculate it with? Agar or LC

How long ago was it pressure cooked? How long since inoculation?


inoculated with agar on the 9th of december and it sat for maybe a day between PC and inoculation.


AZ Starman2112 said:
Curious to what kind of jar you are running your grain in?


This is run in a PP5 container with a hole cut out in the lid and with a filter disc on top. There is a local vendor selling these premade which made it easy to get started and they stack really nicely in a cupboard when not in use. Attentive people might recognize these containers/lids from some of Kloakk's recent post as he has been experimenting with them as well.

Mackload said:
Definitely contaminated.  And same question as above about the jar...


Guess its a throw some coir on top of the jar and top fruit it kind of situation then, we'll see how it recovers.

Edited by DiscoDave (01/13/26 08:43 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29454184 -

DiscoDave said:
To me this jar looks to be bacterial, just shook it and a lot of liquid appeared as well. Was some uncolonized wet grains up against the side before. This is a jar of semperviva if that matters, no off smell coming through the airhole though, and I think there might be some condensation forming from a cold front hitting here. Any thoughts on the jar?




It sure is wet in there, if you just shook it, just let it sit and see if they bounce back, but look toast.

AZ Starman2112 said:
Curious to what kind of jar you are running your grain in?


1 liter PP5 container.



I have used them as AIO's they work okay, but I don't like how the lid open, but besides that they've been fine. One got warped on the second run though so might have to find something else :cryariver:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29454185 -

^ see I’m trying to be positive and hope that day rest between PC and inoculation might just have some moisture that needed redistribution.

But usually clumps of wet are a bad sign of contam.

But let it ride for 5 days and I think confirmation will come from it.


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29454191 -

Good to get a confirmation of it, none of my other jars have turned out this way, so its nice to finally get to see what a bacterial jar looks like

:batlol:

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Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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