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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: markh] * 1
    #29335655 -

markh said:
Is this ok to use with all this sediment in it? I used a premix agar/lme powder and added 1/2 gram of peptides. PC'd for 30 minutes @ 15 psi.




yes

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Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8] * 2
    #29335656 -


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29335657 -

Nichrome is an institution.
sloug said:
markh said:
Is this ok to use with all this sediment in it? I used a premix agar/lme powder and added 1/2 gram of peptides. PC'd for 30 minutes @ 15 psi.




yes


How do you know? Did you test it for them?


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29335658 -

quit genny.  we trying to get this train back on track. for nichromes sake.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29335661 -

Quick q,  what do you take when you have covid or the flu? Feel like i got hit by a truck...


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29335663 -

GenesisCorrupted said:
How do you know? Did you test it for them?


i thnk ur confused. he hasnt even poured it yet. i over cook my agar sometimes and it comes very similar to that. works fine.

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Re: Ask Quick Questions, Get Quick Answers [Re: el53]
    #29335664 -

el53 said:
the field fill up in the misty morning after a cold rainy night which is all you are simulating


no we are not.

we are improving nature not replicating. nature doesnt grow canopies.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29335665 -

tryptkaloids said:
Quick q,  what do you take when you have covid or the flu? Feel like i got hit by a truck...


kratom


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 1
    #29335666 -

sloug said:
GenesisCorrupted said:
How do you know? Did you test it for them?


i thnk ur confused. he hasnt even poured it yet. i over cook my agar sometimes and it comes very similar to that. works fine.


hes just bein a smart ass


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8]
    #29335667 -

1NFIN8 said:
the_chosen_one said:
Check out threads searching 'reservoir tek'. It's no commonly used now a days, but it sounds like you are thinking of an automated version.


If its not commonly used now was it determined to be too much effort for too little benefit?


Not worth the effort.  Introduces vectors for contamination.  Keep it simple.  There is a reason it is widely accepted and practiced to focus on clean spawn and nailing your bulk substrate hydration content.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29335668 -

Depends on how it hits you. I did lots of electrolyte drinks and vitamin c to rehydrate from lost fluids. Get well soon.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 3
    #29335674 -

mushboy said:
el53 said:
the field fill up in the misty morning after a cold rainy night which is all you are simulating


no we are not.

we are improving nature not replicating. nature doesnt grow canopies.



ya if gods so great why is all his shit contaminated?  dumbass cant even grow a canopy


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 2
    #29335680 -

:whathesaid:

god obviously cant vent a pc for shit :noway2:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 4
    #29335682 -

he probaby gets all his info on reddit.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29335683 -

:tubgirl:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29335684 -

Seriously I admire the engineering of the build. It was like Tim the toolman built his version of a PC but there is no chance I want to be less than miles from where it is running since I would never clam to know enough about metal fatigue to find it comfortable.  actually accidentally bought an instant type cooker this week that has a 15 psi setting. How do you accidentally buy another pressure cooker you may ask. I intended to buy a backup dehydrator since mine is the original Ronco and older than you. I went to a closeout warehouse and there was a display dehydrator on top of a stack of brown shipping boxes so I grabbed one. Nothing on the outside indicated the contents. Just getting it in a cart was a struggle since it was literally on a shelf over my head. I'll be taking it to a former colleague for has access to a data logger with a max out of over 400 degrees. Stick that puppy into the middle bag the way I sack them with a small flow of steam space and actually know when it hits sterilization temps as well as how much higher the 15 psi sends it below 1,000 feet elevation.


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“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

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Re: Ask Quick Questions, Get Quick Answers [Re: el53] * 1
    #29335687 -

wut


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29335688 -

BeefSupremeJr said:
i dont think so.  i bet the syringe is fine as they mostly are and im guessing the member print is even finer. so lets see if we can get snyack to test this for us.



The spore syringe is SporeWorks, which I (and it seems others) have a pretty good experience with so.. whatever that means. But prints aren't sterile, and my understanding was that we wouldn't expect a spore syringe to be sterile either?

Either way, I'm fully prepared to test whatever!

Quote:
I didnt want to make that post any longer but thats exactly what i was talking about yesterday. 

i do not think he would have had all that bacteria from a vendor syringe had he just skipped this whole streaking thing and kept it simple. 



I hear you loud and clear. I was attempting to follow what I thought was best practice. Live and learn. That being said, I'm a little surprised that rubbing a flame sterilized bit of wire around the plate created that much of a mess. It doesn't feel like there's enough opportunity to screw that up THAT bad.. particularly in front of a hood. Also, I've tested my hood multiple times by leaving a plate wide open in front of it while I'm working on other plates. And yet, clearly something is going wrong specifically when I'm streaking spores. So if it's not from the syringe and/or the print... ¯\_(ツ)_/¯ but it's obviously coming from somewhere so fuck yeah let's science this.

Quote:
santizie the syringe with iso.  flame your needle, then go into the sab. squirt a drop to cool the needle. 

left hand opens the plate a just a crack while the  right hand puts a few drops in the center of the plate.  close the plate with the lid thats STILL in your left hand.  put the syringe down and wrap the plate. 


OK so this is pretty much my existing process with one notable exception: I don't use the needle. I just remove the luer-lock cap from the end of the syringe, let a few drops out, then put the cap back on. Well, and I do it in front of a hood instead of SAB. It makes me wonder if there's bacteria potentially on the threaded area of the syringe that is not getting sanitized. So before I do this test, I'm going to literally soak the front half of the syringe in ISO for 20m. That seems like it should eliminate that variable, right?

My problem with using the needles is I find it really hard to dispense single drops. I either get nothing, or it squirts too much liquid. And when I do it on LC syringes the needles get plugged with clumps. Meanwhile, I've used the same process to test LC syringes and had none of these issues.

Quote:
thats it.  thats the job.  i always do two plates but i never needed the second. 

if that grows bacteria, throw the syringe away and never buy from that vendor again.


This will be my test. I will do two plates from the spore syringe, and two more plates from the print.

Now the real test, is the streaking really the problem?  LET'S FIND OUT!



One more question though, how do you want me to do the print--scrape the foil with the scalpel over the middle of the open plate and then just close the plate? I don't actually have sterile swabs so I can't swab the print then swab the agar...


--------------------
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"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 3
    #29335689 -

BeefSupremeJr said:
he probaby gets all his info on reddit.


:lolsy:
He used ChatGPT to make the platypus.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29335695 -

You obviously never been picking any place decent. Nature certain makes canopies.


--------------------
“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

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