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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29334428 -

That's true to. You're right about that.    That's the multiple views thing in play tho.  At some point they're always becomes a discussion when there is a disagreement or difference in view. And then everybody learns either way.

Kinda like us right now



Maybe they should change the thread title and add short discussion. Seeing as it does happen pretty often.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: BlueNicie]
    #29334430 -

BlueNicie said:
Thanks for the info, was thinking it could be some type of flaw in the bottle but just wasn’t sure if it could have been something else. I will definitely add a towel next time. The first time was totally my fault because I forgot to loosen the lid, I am guessing that was really an implosion since it went into cold water but it freaked me out, glass hitting my closed eyelid. I will probably just use a water bath next time and stop trying to take short cuts. I always used to put my elio’s pizzas in the microwave for a minute then the oven for a few minutes less and that never worked out well either



water bath takes frickin forever to melt solidified agar. no need to fridge it either. if u do try microwave again, keep lid loose while heating and tighten when u pull it out. never put hot glass in cool water with lid tight OR loose, it will crack. and do rag/towel tek just to be safe

rock on!

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29334436 -

callmehippie said:
That's true to. You're right about that.    That's the multiple views thing in play tho.  At some point they're always becomes a discussion when there is a disagreement or difference in view. And then everybody learns either way.

Kinda like us right now



Maybe they should change the thread title and add short discussion. Seeing as it does happen pretty often.



yea i realized i was doing it is why i said anything. im going to try to keep my answers here short and concise from here on out. if everyone did that without bickering back and forth, the person asking the question could just use their best judgement from the viewpoints given

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29334437 -

Henry Swanson said:
BeefSupremeJr said:
qq I ever tell you guys I dont believe in streaking?

yeah i think its not actually helpful in this hobby.  i mean why reduce the genetic material to work with? i think its pointless in our circumstsnce and doesnt accomplish anything a few transfers couldnt anyway.  its just fancy. 

i mean read anyones process and theyll explain how its done and at the most theyll explain how it helps dilute and isolate cultures.  but why?  WHY do we want that?

i want to go to fruit with as much genetic material as possible.  it youre playing the lottery, does it not make sense to have as many tickets as possible? i mean i also think playing lottery is kindof stupid but mushroom pheno hunting is a litlte more like playng slots.  okay, does it not make sense to play as many machines as possible?

well? does it?



Again this wasn’t being shared to imply using it everytime is necessary or to try and isolate cultures. It was specifically to advise on a method that can help clean up known dirty spores / LC. Most of my focus has been on exotics and I sometimes work with wild spores. The method definitely helps with getting colonies of clean myc. It’s not the only way, just a tool to keep in the tool box. Just like using brf pucks, cabin sequestering ect…



speaking of long-winded answers..

i can totally get behind this.  totally, its a tool if youre dealing with a bacterial spore source.  for sure! (at least a simplified quick version of it)
a simple zig zag > the pumping arms in and out of a sab with a pate laying spread eagle in a homemade sab.  probably i cant be convinced otherwise.

i frankly think streaking is one of these applied sciences techniques that might do more harm than good for new members.  New growers dont have $20k flow hoods like we use in college/pro laboratories and the insistence on these applied science techniques serves as a barrier for non-scientists to get into agar.  its still a struggle getting new members to think of agar as something easy when we link them to this stuff.  old long-gone members talking about the importance of isolates and such. 

As of now, streaking remains in the hhg as it still seems to be the convention and as i remain a currator of our collective convention, ill probably leave it that way, but on a personal level, i dont agree with it as a standard protocol to make a germ plate. 

I think it should be considered a protocol IF and WHEN we find bacteria and we really want to keep the culture. 

A simple drop of spores and then trasferring LARGE wedges to new plates is just easier and has vastly fewer vectors of contam.  shit, ive RAN germ plates so many times i cant keep count.  (after i take a wedge as a backup)

its a different perspective.  taking a wedge and keeping the germ plate as a backup vs taking a wedge as a backup and running the germ plate...

moreover i think cabin/trenching is every bit as effective i terms of cleaning up a culture.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 2
    #29334441 -

I can dig it. :rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 5
    #29334456 -

I like to take the tip of my loop, lightly touch the print, draw a Z.

I pride myself on brevity.

This is why I can't stand watching instructional videos. All the blab wasting my time.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29334459 -

BeefSupremeJr said:
pyrex media bottle?  hmm someone told me pyrex isnt what it once was. as in, not always actual borosilicate.  it really shouldnt break like that.



I have a funny, recent story that happened just the other day involving 500ml Pyrex media bottles.  So, I had just finished pouring plates and was washing the bottles out.  Now keep in mind, my entire down stairs is tile floors, but anywho, the media slipped out of my hands, upwards trajectory, over the sink about 7’ in the air.  It’s flying in the air as I stare at it and goes about 10’ to the center of the living room and hits the tile. As I cringed, this sucker bounces off the tile, back up to 6’ and back down, bounces again and again until it settled down and rolled under the couch.  I cannot even believe that it didn’t break, I mean the force of hitting that tile!  I’m so glad it didn’t break but it is a testament to the quality of the glass or dumb luck, 🤷‍♂️.  Oh and it was the ‘Zon glass!

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29334463 -

lol thats a fuckin warner bros cartoon. 

then theres my dumbass i mixed kratom in it for the road. tossed it in the back cab of truck and when i opened the back door to grab something at a jobsite it fell out and shattered in front of my client.  he asked why i needed media bottles and i said

"water samples"

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29334466 -

Bahaha! 🤣

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29334468 -

corning still makes pyrex scientific glass. its quality

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 2
    #29334472 -


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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 2
    #29334479 -

sloug said:
well in the op's defense, there is an entire forum for discussions to be had. this thread is not a "chat room" so to speak. it was meant to be quite the opposite



It isn't a chat room. You're right. However, I will not appologize for being personable or social or having fun. Yes, you've been here a few years longer than I, and I respect that. However, threads evolve and become what they are. They ebb and flow. On cultivational confessions we had a disscussion yesterday about personal grooming as in shaving ones junk. Does it belong there? Probably not. However, it was friendly civil talk, fun and funny. So there it lays in the ebb & flow of the thread.  Personally I'll never criticize anyone for asking a question with an explanation, no matter how long it is. As I said, some people can't help this and it is the way they communicate. What worries me is someone reading this that wants to ask a question and now might not because they are afraid it might be to long to ask. The only bad question is the one that isn't asked.

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29334483 -

nichrome said "try" to keep things breif.  i try, i just suck at it. i want to be like ldk when i grow up.

also btw, confessional booth is basically the otd of the cult forum.  other members are known for being concise or succinct but im known for having a baren garden of fucks. i do try though.

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 2
    #29334485 -

no disrespect. im still very new here in terms of posting. just an observation n suggestion

        :sunny:
:boombox2:

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29334492 -





There are two little white growths right at the water level. Is that contamination? The mycelium growing at the bottom seems nice and healthy.

Also I have a second jar of enigma that almost has no growth and the LC is cloudy. Is that contaminated?

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Re: Ask Quick Questions, Get Quick Answers [Re: ihansterx4i]
    #29334495 -

LC is a bit difficult to see contams, and remember, growth mats do develop on the surface, usually later on as the LC sits.  It may be worth it to drop some on a couple plates to check, but each time you open the jar is another vector for contams.

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29334545 -

Synack
Quote:
Splittinghairs said:



Help identifying please.

Second time I’ve ordered from a vendor and NOT gotten APEs but this doesn’t look like PE either.



Make sure you are using a trusted shroomery sponsor. APE was developed by our very own Workman (dont pm him looking for spores, he runs a business.)


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: ihansterx4i]
    #29334546 -

ihansterx4i said:




There are two little white growths right at the water level. Is that contamination? The mycelium growing at the bottom seems nice and healthy.

Also I have a second jar of enigma that almost has no growth and the LC is cloudy. Is that contaminated?



Every time i see floaters like that i assume mold. Always test lc before use.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 6
    #29334555 -



Sometimes ya really do just gotta remember to stir this shit though lol


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 1
    #29334557 -

hydroponic shrooms
:yields:

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